Fig. 5: Effect of Euro 5 and Euro 4 DEPs, before any after-treatment, in enhancing citrullinated protein levels in Normal Human Bronchial Epithelial (NHBE) cells. | Cell Death & Disease

Fig. 5: Effect of Euro 5 and Euro 4 DEPs, before any after-treatment, in enhancing citrullinated protein levels in Normal Human Bronchial Epithelial (NHBE) cells.

From: Diesel exhaust particles induce autophagy and citrullination in Normal Human Bronchial Epithelial cells

Fig. 5

a Western blot analysis of citrullinated protein levels in lysates from NHBE cells (i) untreated and cultured for 48 h with (ii) Euro 5 3.3 μg/cm2, (iii) Euro 4 3.3 μg/cm2, (iv) Euro 5 6.6 μg/cm2, (v) Euro 4 6.6 μg/cm2. Blots shown are representative of three experiments (N = 3). b Cells were lysed and vimentin was immunoprecipitated from lysates. An appropriate IgG isotypic control (irrelevant IgG, Irr. IgG) was employed to verify that immunoprecipitation was correctly performed. Blots shown are representative of nine experiments (N = 9). c Densitometry analysis of citrullinated vimentin levels relative to vimentin (mean ± SD). For 3.3 μg/cm2: P = 0.0073 Euro 5 vs untreated; P = 0.0025 Euro 4 vs untreated; P = 0.0467 Euro 5 vs Euro 4. For 6.6 μg/cm2: P < 0.0001 Euro 5 and Euro 4 vs untreated; P = 0.0119 Euro 5 vs Euro 4. For a dose-depending comparison: P = 0.0003 Euro 5 3.3 vs 6.6 μg/cm2; P = 0.0009 Euro 4 3.3 vs 6.6 μg/cm2

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