Fig. 5: Engraftment and non-tumorigenicity of hiNSCs upon transplantation into the brain. | Cell Death & Disease

Fig. 5: Engraftment and non-tumorigenicity of hiNSCs upon transplantation into the brain.

From: Establishment of stable iPS-derived human neural stem cell lines suitable for cell therapies

Fig. 5

a Haematoxilyn-eosin stain of ipsilateral hemisphere of animals transplanted with i hiNCSs (n = 11), ii hNSCs (n = 10) and iii hGBM cells (n = 3). b Brain map showing the distribution of transplanted i hiNSCs and ii hNSCs (huN+, green) throughout the brain. In the magnifications are shown the brain regions evaluated for quantification analysis: cortex, striatum, corpus callosum, subventricular zone (not shown) and injection site. c Quantification of i migration, ii survival ability and iii of the relative percentages of neurons, astrocytes and oligodendrocytes generated by hiNSCs and hNSCs. d Confocal images showing the expression of i proliferation (KI67, red), ii neural (huNES, green), iii astroglial (GFAP, red), iv neuronal (TUBB3, red) and v oligodendroglial (MBP, red) markers for hiNSCs and hNSCs (huN+, green). Nuclei are shown by TO-PRO-3 staining (blue). Scale bar: a, b = 1000 µm, d = 75 µm, d, i, ii, v inserts = 17–19 µm, d, iv insert = 10 µm