Fig. 4: Safe expansion of hiNSC lines. | Cell Death & Disease

Fig. 4: Safe expansion of hiNSC lines.

From: Establishment of stable iPS-derived human neural stem cell lines suitable for cell therapies

Fig. 4

a Growth factor-deprivation curves demonstrating the growth factors dependence of hiNSC lines. b qRT-PCR showing downregulation of hTERT mRNA expression in hiNSCs respect to hiPSCs. Each bar represents the mean ± SEM of three independent experiments for each clone (hiPSCs and hiNSCs) in duplicate. Data are expressed as log10 fold changes (f.c.) respect to non-nucleofected fibroblasts (Fibrowt) and are normalised on actin expression. c Comparison between the growth curves (i) and their slope (ii) of hiNSC (n = 15), respect to brain-derived hNSCs (n = 32) and hGBM (n = 12)