Fig. 2: Generation and characterisation of hiNSCs. | Cell Death & Disease

Fig. 2: Generation and characterisation of hiNSCs.

From: Establishment of stable iPS-derived human neural stem cell lines suitable for cell therapies

Fig. 2

a Schematic diagram illustrating the overall strategy to generate hiNSCs from hiPSCs. Phase contrast images illustrate the typical morphology of cells at each stage. b Bar graphs of qRT-PCR showing mRNA-expression profile of hiNSCs, hiPSCs and brain-derived hNSCs respect to Fibrowt. c Bar graphs of qRT-PCR showing mRNA-expression profile of EBs, hiNSCs and hNSCs respect to hiPSCs. d Bar graphs of qRT-PCR showing mRNA-expression profile of hiNSCs and hNSCs respect to hiPSCs. Each bar represents the mean ± SEM of three independent experiments in duplicate. Data are expressed as log10 fold changes (f.c.) respect to non-nucleofected fibroblasts (b, Fibrowt) or hiPSCs (c, d). Data are normalised on actin expression. Scale bars: a, i = 50 μm; a, ii–v = 100 μm