Fig. 5: ACY-1215 promoted miR-30d expression, and miR-30d directly downregulated PI3K. | Cell Death & Disease

Fig. 5: ACY-1215 promoted miR-30d expression, and miR-30d directly downregulated PI3K.

From: Ricolinostat (ACY-1215) suppresses proliferation and promotes apoptosis in esophageal squamous cell carcinoma via miR-30d/PI3K/AKT/mTOR and ERK pathways

Fig. 5

a ACY-1215 treatment significant increased miR-30d expression in EC109 and TE-1 cells for 48 h (50 μM). b ACY-1215 treatment significant reduced PIK3R2 expression in EC109 and TE-1 cells for 48 h (50 μM). c, d Cells were treated with Lipofectamine 2000, 50 μM ACY-1215, and/or 50 nM miR-30d inhibitor for 48 h. The quantitative real-time PCR analysis demonstrated that PIK3R2 expression significantly increased after cells were transfected with miR-30d inhibitor, particularly in the ACY-1215 treatment group. The results are shown as the mean ± SD. **p < 0.01; ***p < 0.001

Back to article page