Fig. 6: Imbalance of LIMK1/LIMK2 promotes CRC aggression via regulating WNT/β-catenin signaling pathway. | Cell Death & Disease

Fig. 6: Imbalance of LIMK1/LIMK2 promotes CRC aggression via regulating WNT/β-catenin signaling pathway.

From: Imbalanced LIMK1 and LIMK2 expression leads to human colorectal cancer progression and metastasis via promoting β-catenin nuclear translocation

Fig. 6

a Wound-healing assay of cells overexpressing LIMK1 and co-transfected with siLIMK2 or LIMK2 plasmid, as indicated. b Transwell assay of cells overexpressing LIMK1 and co-transfected with siLIMK2 or LIMK2 plasmid, as indicated. The cells were counted under a microscope in five randomly selected fields. Bars represent the number of invaded cells. c Whole proteins from LIMK2 knocked down cell lines, co-transfected with LIMK1 vector cells, and co-transfected cells treated with the β-catenin inhibitorXAV939 (5 μM) or DMSO for 24 h, were used for detecting the indicated proteins of the Wnt/β-catenin pathway by WB. d IF assays verified β-catenin abundance in LIMK2 knocked down cell lines, co-transfected with LIMK1 vector cells, and co-transfected cells treated with XAV939 (5 μM) or DMSO for 24 h. Representative merge figures are shown (magnification, × 400; scale bar: 20 µm). e LIMK2 knocked down cell lines, co-transfected cells, and co-transfected cells treated with the XAV939 (5 μM) were measured migration ability by Transwell assays. Error bars represent mean ± S.D. from three independent experiments. ***P<0.001

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