Fig. 5: Imbalance of LIMK1/LIMK2 promotes β-catenin nuclear accumulation . | Cell Death & Disease

Fig. 5: Imbalance of LIMK1/LIMK2 promotes β-catenin nuclear accumulation .

From: Imbalanced LIMK1 and LIMK2 expression leads to human colorectal cancer progression and metastasis via promoting β-catenin nuclear translocation

Fig. 5

a IHC staining of LIMK1, LIMK2 in 27 normal and CRC tissues,17 SA tissues. Representative figures are shown (magnification, × 200; scale bar: 50 µm). b Real-time PCR analysis of LIMK1 and LIMK2 in 40 normal mucosa tissues. Correlation analysis were shown by Spearman’s correlation analyses between LIMK1 expression and LIMK2. c Expression of LIMK1 was detected by WB in CRC cell lines with LIMK2 knocked down or overexpressed, respectively. d IF analysis for subcellular localization of LIMK1-HA and LIMK2-Flag in LoVo cells (magnification, × 400; scale bar: 20 µm). e Nuclear proteins from SW480 and SW480 transfected with LIMK1, transfected with LIMK2 or siLIMK2, respectively, were used for detecting the indicated proteins of the LIMK1, LIMK2 and β-catenin by WB. f Immunofluorescence assays of LIMK2 and β-catenin proteins in SW480-transduced cells, as indicated. Representative merge figures are shown (magnification, × 400; scale bar: 20 µm)

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