Fig. 2: Reduced LIMK2 contributes to aggressive phenotypes of cell lines in vitro. | Cell Death & Disease

Fig. 2: Reduced LIMK2 contributes to aggressive phenotypes of cell lines in vitro.

From: Imbalanced LIMK1 and LIMK2 expression leads to human colorectal cancer progression and metastasis via promoting β-catenin nuclear translocation

Fig. 2

a, b SW480 cells were transiently transfected with siLIMK2 and siRNA NC. LoVo cells were transiently transfected with LIMK2 and NC vectors. Western blot analysis was performed to detect the expression of LIMK2. c, d Wound-healing assays were used for detecting the migration ability in LIMK2 knocked down or overexpression CRC cell lines. Bars represent migration index of treated or control cells. The distance migrated by treated cells was relative to that migrated by control cells. Representative figures are shown. Error bars represent mean ± S.D. from three independent experiments. The asterisk (*) indicates P<0.05. The double asterisk (**) indicates P<0.01. e, f The invading cells of the transwell assay were counted under a microscope in five randomly selected fields. Bars represent the number of invaded cells. Error bars represent mean ± S.D. from three independent experiments. ***P<0.001

Back to article page