Fig. 1: There are relationships among expressions of HS6ST2, MMP13, and miR-23b-3p in OA-damaged cartilage. | Cell Death & Disease

Fig. 1: There are relationships among expressions of HS6ST2, MMP13, and miR-23b-3p in OA-damaged cartilage.

From: Downregulation of HS6ST2 by miR-23b-3p enhances matrix degradation through p38 MAPK pathway in osteoarthritis

Fig. 1

a Representative macromorphological pictures of smooth cartilage (upper, left panel) and damaged cartilage (upper, right panel) from OA patients and representative Safranin O-fast green-stained histologic specimens of smooth OA cartilage and damaged OA cartilage. Scale bar: 50 μm. b Relative expression of HS6ST2 mRNA (left panel) and MMP13 mRNA (right panel) in damaged cartilage compared with smooth cartilage from the same OA patients (n = 18) were determined by RT-qPCR. c Left, representative immunohistochemical localization of HS6ST2 expression in OA smooth and damaged cartilage from same patient. Arrows stand for HS6ST2-positive cells. Right, statistical analysis of average optical density in immunohistochemical detection from OA patients (n = 16). Scale bar, 100 μm. d Relative expression of miR-23b-3p in damaged cartilage compared with smooth cartilage from the same OA patients (n = 18) were determined by RT-qPCR. e The correlation analysis between miR-23b-3p and MMP13 mRMA expression from OA patients was performed. f, g The correlation analysis between miR-23b-3p and HS6ST2 expression from OA patients was performed. U6 snRNA was used as an internal control in RT-qPCR for miRNA and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was chosen for mRNA detection. Paired t-test was used to identify differences statistically between OA smooth cartilage group and OA-damaged cartilage group. *P < 0.05 and **P < 0.01. The correlation analysis was performed by using Spearman correlation

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