Fig. 4: CYLD downregulation is responsible for upregulation of RIPK1 in melanoma cells with acquired resistance to BRAF inhibitors. | Cell Death & Disease

Fig. 4: CYLD downregulation is responsible for upregulation of RIPK1 in melanoma cells with acquired resistance to BRAF inhibitors.

From: RIP1 protects melanoma cells from apoptosis induced by BRAF/MEK inhibitors

Fig. 4

a, b Total RNA from melanoma cells was subjected to qPCR analysis. Data are mean ± SE, n = 3. *P < 0.05, Student’s t test. c Whole-cell lysates from cells treated with cycloheximide (CHX; 1 μg/ml) for indicated periods of time were subjected to western blotting. d Quantitation of the western blot bands as shown in c. e, f RIP1 immunoprecipitates from cells treated with MG132 (5 μM) for 6 h were subjected to western blotting. g Whole-cell lysates were subjected to western blotting. h RIP1 immunoprecipitates from whole-cell lysates were subjected to western blotting. i, j Cells individually transfected with either Flag-HA-GFP or Flag-HA-CYLD were co-transfected with Lv201CT Vector or pEZ-Lv201-RIP1. Forty-eight hours after transfection, whole-cell lysates were subjected to western blotting (i), cells were subjected to the apoptosis assay with Annexin V-FITC and PI (j). Data are mean ± SE, n = 3. *P < 0.05, **P < 0.01, ***P < 0.001, Student’s t test. k Whole-cell lysates from cells transfected with either control siRNA or CYLD siRNA were subjected to western blotting

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