Requirement of GSK-3 for PUMA induction upon loss of pro-survival PI3K signaling

Growth factor withdrawal induces rapid apoptosis via mitochondrial outer membrane permeabilization. We had previously observed that cell death of IL-3-dependent Ba/F3 cells, induced by removal of the growth factor, required the activity of the kinase GSK-3. Employing CRISPR/Cas9-mediated gene knockout, we aimed to identify pro-apoptotic GSK-3 regulated factors in this process. Knockout of either Puma or Bim demonstrated that the induction of Puma, but not Bim, was crucial for apoptosis induced by IL-3 deprivation. Thus, we aimed at identifying the GSK-3-dependent PUMA regulator. Loss of FOXO3A reduced the induction of Puma, while additional loss of p53 completely repressed induction upon growth factor withdrawal. A constitutively active mutant of FOXO3A, which cannot be controlled by AKT directly, still required active GSK-3 for the full transcriptional induction of Puma and cell death upon IL-3 withdrawal. Thus, the suppression of GSK-3 is the key function of PI3K signaling in order to prevent the induction of Puma by FOXO3A and p53 and thereby apoptosis upon growth factor withdrawal.


Introduction
Growth factor signaling supports cell survival through various pathways. Thus, deprivation of growth factor ultimately results in apoptosis. The decisive step for the induction of intrinsic apoptosis is the mitochondrial outer membrane permeabilization (MOMP). This results in the release of cytochrome c and other proteins from the mitochondrial intermembrane space into the cytosol, leading to apoptosome formation, caspase activation, and apoptosis. MOMP is controlled by proteins of the BCL-2 family. While the pro-apoptotic BCL-2 proteins BAX and BAK are required for the formation of a mitochondrial outer membrane pore, their activity is induced by BH3only proteins (PUMA, BIM, Bid, and others). MOMP is prevented by related proteins with anti-apoptotic function (like BCL-2, MCL-1, BCL-xL) 1 .
MOMP is controlled by growth factor availability, which induces various pathways promoting cell survival. A key pro-survival pathway is the PI3K/AKT signaling pathway, which can prevent MOMP and apoptosis through regulating a number of substrates. For instance, AKT was shown to phosphorylate and inactivate the transcription factor FOXO3A as well as glycogen synthase kinase-3 (GSK-3). The inactivation of both FOXO3A and GSK-3 was shown to play an important role for the pro-survival activity of PI3K/AKT signaling [2][3][4] . More specifically, it was shown that the suppression of FOXO3A plays an essential role for the suppression of Puma induction and cell death by PI3K signaling 5 .
The death promoting role of GSK-3 is instrumental for p53-mediated Puma induction and apoptosis: GSK-3 phosphorylates the histone acetyl transferase Tip60 (also known as KAT5), which stimulates Tip60 to acetylate p53 at K120, resulting in the transcriptional induction of Puma and apoptosis upon induction of p53 6 . Interestingly, GSK-3 was also shown to modulate the transcriptional activity of FOXO3A 7,8 .
In the present study, employing knockout by CRISPR/ Cas9, we systematically investigated the role of GSK-3dependent factors required for apoptosis induction by IL-3 deprivation. We show that PUMA is the main proapoptotic protein responsible for apoptosis in this context, and that the induction of Puma is mediated by a FOXO3A-, p53-, and GSK-3-dependent mechanism.

Results
Apoptosis induced by growth factor withdrawal requires GSK-3-dependent PUMA induction When IL-3-dependent cells such as Ba/F3 or FL5.12 cells (two murine pro B cell lines) are deprived of the growth factor, they undergo rapid apoptosis. Additional treatment with the highly selective GSK-3 inhibitor CT98014 completely blocked IL-3-withdrawal-induced apoptosis of Ba/F3 cells as observed previously 9 (Fig. 1a). We aimed at systematically defining the pro-apoptotic factors involved in IL-3 withdrawal-induced apoptosis and at investigating their link to GSK-3. To address the role of pro-apoptotic BH3-only proteins for growth factorwithdrawal-induced apoptosis, we transduced Ba/F3 cells with the lentiCRISPRv2 system targeting either Puma or Bim. As shown in Fig. 1b, apoptosis by IL-3 deprivation was substantially reduced in Ba/F3 cells expressing CRISPR/Cas9 targeting Puma, while loss of Bim conferred only moderate protection from cell death. This effect was even more pronounced in the IL-3dependent cell line FL5.12 (Fig. S1A). To further verify the role of PUMA in this system, clones derived from individual cells (single-cell clones) were generated from the CRISPR/Cas9-transduced cultures and cells with frameshift mutations on both Puma alleles or both Bim alleles were selected. Almost all Puma −/− single-cell clones were strongly protected from IL-3 withdrawal-induced apoptosis ( Fig. 1c) while Bim −/− single-cell clones exhibited no statistically significant survival advantage (Fig. 1d). The protective effect of Puma depletion lasted at least 24 h, however, the cells committed to apoptosis at later time points. Puma −/− Ba/F3 cells additionally treated with the GSK-3 inhibitor CT98014 showed a similar kinetic but the protective effect was more pronounced even after 40 h (Fig. S1B). Thus, the kinase activity of GSK-3 as well as the induction of Puma are rate-limiting for apoptosis induced by IL-3 withdrawal. The stronger anti-apoptotic effect achieved by inhibition of GSK-3 than by the loss of PUMA alone is consistent with other cell death regulatory factors being regulated by GSK-3, such as MCL-1 9 (Fig. S1C). We next asked whether PUMA is transcriptionally regulated in a GSK-3-dependent manner upon growth factor withdrawal. Wild-type Ba/F3 cells were deprived of IL-3, treated with GSK-3 inhibitor (CT98014) and Puma mRNA levels were analyzed by quantitative RT-PCR. IL-3 withdrawal-induced Puma mRNA up to 2-fold after 7.5 h while Puma mRNA was reduced upon treatment with CT98014 in the absence of IL-3 (Fig. 1e). This effect was reflected by the protein levels of PUMA in Ba/F3 wt cells: PUMA was induced upon IL-3 withdrawal, but this upregulation was completely blocked by addition of CT98014 (Fig. 1f). Loss of PI3K is permitting GSK-3 activity by relieving the suppression of GSK-3 by AKT-mediated phosphorylation. Consistently, we found that the pharmacological inhibition of PI3K resulted in strong induction of PUMA (Fig. S1D).
We next generated IL-2-dependent murine primary lymphocytes, which were deprived of the growth factor. Consistent with the results obtained with IL-3-dependent cell lines, removal of IL-2 induced PUMA and apoptosis, while this was abrogated in presence of the GSK-3 inhibitor (Fig. S1E, Fig. S1F).
We therefore conclude that PUMA represents the main BH3-only protein mediating IL-3 and IL-2 withdrawalinduced apoptosis induction and that the transcriptional induction of Puma is dependent on GSK-3 activity.
p53 has a minor role for GSK-3-dependent PUMA induction We next investigated the identity of the transcription factor, which mediates the GSK-3-dependent transcriptional induction of Puma upon loss of growth-factorinduced PI3K signaling. Puma is a crucial pro-apoptotic target of p53 10,11 . While p53 is not stabilized upon growth factor withdrawal, p53 is nevertheless able to induce some of its target genes (such as Mdm2) when present at very low levels. Thus, even in the absence of DNA damage, we considered p53 as a candidate for GSK-3-dependent Puma induction upon loss of pro-survival signaling during IL-3 withdrawal. To address the role of p53 in this context, we inhibited PI3K by GDC-0941 in HCT116 p53 +/+ and p53 −/− cells in order to induce PUMA, as described before (see Fig. S1D). As shown in Fig. 2a, inhibition of PI3K-induced PUMA in p53 +/+ as well as p53 −/− cells, indicating that p53 is not a major transcription factor responsible for Puma induction in this setting. Consistent with the data shown before, the induction of PUMA was prevented by pharmacological inhibition of GSK-3. To further investigate the role of p53, we knocked out p53 by CRISPR/Cas9 in Ba/F3 cells, which have an intact p53 signaling pathway 6 . Upon IL-3 withdrawal, Ba/F3 cells lacking p53 exhibited some protection from apoptosis (Fig. 2b), which was however not as strong as observed with cells lacking PUMA (see Fig. 1b). We generated p53 −/− single-cell clones and subjected them to IL-3 deprivation. We unexpectedly observed that individual Ba/F3 cell clones lacking p53 exhibited quite some variation regarding the protection from IL-3 withdrawal-induced apoptosis. Nevertheless, on average, the protection conferred by lack of p53 was p53 -/clones , or a combination of both for 7 h. The cells were harvested, subjected to western blotting and analyzed by the antibodies indicated. b Ba/F3 expressing CRISPR/Cas9 targeting p53 or Luciferase were deprived of IL-3, in presence or absence of CT98014 (0.75 µM) for 18 h and analyzed for apoptosis by Annexin V staining. Error bars represent SD from technical replicates. c Ba/F3 p53 −/− single-cell clones and Ba/F3 expressing CRISPR/Cas9 constructs targeting Luciferase were deprived of IL-3 for 18 h and analyzed for apoptosis by Annexin V staining. Each dot represents the mean of two independent experiments analyzing an individual single-cell clone. Error bars represent 95% confidence interval from two independent experiments (n = 2). Significance was tested by one-way ANOVA with post hoc Tukey's multiple comparison test. ns = not significant. d Ba/F3 p53 −/− single-cell clones and two independent cell lines of Ba/F3 cells infected with CRISPR/Cas9 targeting Luciferase were deprived of IL-3 for 12 h, harvested and analyzed by western blotting by probing with antibodies as indicated. Bands were quantified using FusionCapt Advance Solo 4 16.08. PUMA levels were normalized to GSK-3 levels (loading control). e Ba/F3 p53 −/− single-cell clones and two independent cell lines of Ba/F3 expressing CRISPR/Cas9 constructs targeting Luciferase were treated with LY294002 (10 µM) or DMSO (−) for 18 h, harvested and analyzed by western blotting with the antibodies indicated. Bands were quantified using FusionCapt Advance Solo 4 16.08. PUMA levels were normalized to GSK-3 levels (loading control). modest (Fig. 2c). Interestingly, a variation in IL-3-induced apoptosis was also observed in different single-cell clones expressing CRISPR/Cas9 targeting Luciferase and-of note-in wild-type Ba/F3 cells, indicating that individual clones always seems to exhibit some variation, even in the absence of previous manipulation. We therefore always analyzed a high number of clones (Fig. S2A+B). Consistent with the modest protection of p53 −/− single-cell clones from IL-3 deprivation, PUMA induction was still observed in p53 −/− single-cell clones upon IL-3 withdrawal (Fig. 2d) or PI3K inhibition by LY294002 (Fig. 2e) and was still dependent on GSK-3 (Fig. S2C). However, the quantification of PUMA expression levels revealed that, while Puma was still induced, the PUMA protein levels were generally somewhat reduced in p53 −/− cells ( Fig. 2e, right). Together, although there is a GSK-3dependent effect of p53 on PUMA and apoptosis induction, p53 seems not to be the main GSK-3-dependent transcription factor responsible for the induction of PUMA and apoptosis upon IL-3 withdrawal.

FOXO3A is an important Puma inducer upon growth factor deprivation
To test other transcription factors for their role in inducing Puma upon IL-3 deprivation, we generated Ba/ F3 expressing CRISPR/Cas9 targeting Foxo1, Foxo3a, and the p53 relatives p63 and p73. We then subjected these cells to IL-3 withdrawal and analyzed apoptosis induction. Among the cells we tested, only Foxo3a-targeted cells showed a protection from IL-3 deprivation (Fig. 3a,  Fig. S3A). Single-cell clones generated from these cells again showed a wide distribution of sensitivity to IL-3 withdrawal but were on average significantly protected compared to control cells (Fig. 3b). The Foxo3a −/− singlecell clones showed a diminished capacity to induce PUMA upon IL-3 withdrawal (Fig. S3B). Because of the high variation of individual Foxo3a −/− single-cell clones to induce PUMA, they were pooled and we observed a clear reduction of the signal for PUMA upon IL-3 withdrawal (Fig. 3d). Likewise, Foxo3a −/− single-cell clones treated with the PI3K inhibitor LY294002 exhibited reduced Puma induction, as evident from the quantification of the PUMA expression levels (Fig. 3d).

Double knockout of Foxo3a and p53 fully protects cells from IL-3-induced apoptosis and prevents Puma induction
We next addressed the combined role of p53 and FOXO3A to induce apoptosis and PUMA upon IL-3 withdrawal. We generated cells expressing CRISPR/Cas9 targeting p53 and Foxo3a simultaneously and identified two single-cell clones with frameshift mutations on both alleles of each p53 and Foxo3a (double knock out, DKO). As shown in Fig. 4a, both DKO clones were protected from IL-3 deprivation-induced apoptosis. In line with this, PUMA induction was absent upon treatment with LY294002 ( Fig. 4b) or IL-3 withdrawal (Fig. 4c) in these cells. Thus, p53 and FOXO3A together are required for PUMA upregulation and apoptosis upon IL-3 withdrawal, possibly also compensating for each other to some extent in cells lacking either p53 or FOXO3A.
The induction of Puma by FOXO3A depends on GSK-3 To confirm the major role of FOXO3A and its GSK-3 dependency in IL-3 withdrawal-induced apoptosis, we reexpressed FOXO3A with silent mutations, rendering it resistant to CRISPR/Cas9 cleavage, in a Ba/F3 bulk culture which expressed a CRISPR/Cas9 construct targeting Foxo3a (Fig. 5a) as well as in a Foxo3a −/− single-cell clone (Fig. 5b). Re-expression of FOXO3A re-established apoptosis induction to a comparable level as in control cells transduced with FOXO3A in bulk culture and the single-cell clone (Fig. 5a, b). Importantly, restoring FOXO3A and thereby the competence to undergo apoptosis did not relieve the requirement for GSK-3 activity, as inhibition of GSK-3 suppressed apoptosis induced by IL-3 withdrawal in FOXO3A overexpressing cells. Thus, FOXO3A-dependent PUMA and apoptosis induction upon growth factor withdrawal requires GSK-3. Of note, the induction of apoptosis as well as the induction of PUMA in Foxo3a −/− cells was still dependent on GSK-3 ( Fig. S4A+B), confirming that the regulation of PUMA by p53 is also controlled by GSK-3 6 .
While PUMA protein levels were reduced in empty vector-transduced Foxo3a −/− cells upon IL-3 withdrawal, PUMA induction was restored in Foxo3a −/− cells reexpressing CRISPR/Cas9-resistant FOXO3A (Fig. 5c). Consistently, FOXO3A overexpression in HCT116 p53 −/− cells increased PUMA induction in cells treated with the AKT inhibitor GDC-0941 (Fig. 5d). We therefore conclude that FOXO3A induces Puma when Ba/F3 are deprived of IL-3, while p53 contributes to this induction, and that both transcription factors require GSK-3 activity for the induction of PUMA and apoptosis.

FOXO3A requires GSK-3 activity for full transcriptional activity
As shown above, the pharmacological inhibition of GSK-3 was sufficient to prevent growth factor withdrawal-induced PUMA induction and apoptosis. We previously demonstrated that when p53 is stabilized by DNA damage, GSK-3 activity regulates the capacity of p53 to induce Puma by phosphorylating Tip60 at S86, which stimulates Tip60 to acetylate lysine 120 (K120) of p53 6 . K120-acetylated p53 then induces Puma which leads to apoptosis [12][13][14] . However, in p53 −/− cells we observed FOXO3A-dependent PUMA induction and apoptosis, which was also dependent on GSK-3 activity (Figs. 2a-c  and 5d). To investigate the role of GSK-3 for the transcriptional regulation of Puma by FOXO3A, we generated a promoter reporter system where Luciferase expression is under control of the Puma promoter, which includes a binding site for FOXO3A 5 . It is well established that FOXO3A is controlled by the PI3K/AKT pathway as phosphorylation of FOXO3A residues T32, S253, and S315 by AKT results in retention of the transcription factor in the cytosol 2 . We expressed a FOXO3A-triple mutant (TM; T32A-S253A-S315A), which cannot be phosphorylated by AKT and is considered to be  constitutive active, along with the Puma promoter reporter constructs. As shown in Fig. 6a, overexpression of FOXO3A-TM induced Luciferase expression controlled by the Puma promoter. This was dependent on a FOXO3A binding site in the promoter region, as mutation of the binding site for FOXO3A prevented Luciferase induction. However, when we added the GSK-3 inhibitor CT98014, the ability of FOXO3A-TM to induce Luciferase through the Puma promoter was substantially decreased. We therefore conclude that FOXO3A, in order to fully exhibit its transcriptional activity at the Puma promoter, requires not only the dephosphorylation of AKT phosphorylation sites in FOXO3A, but also the activity of GSK-3.
We next established Ba/F3 cell lines overexpressing FOXO3A-TM. We expressed FOXO3A-TM with silent mutations, mediating resistance to CRISPR/Cas9 cleavage, in a Ba/F3 bulk culture expressing a CRISPR/Cas9 construct targeting Foxo3a, or in control cells with CRISPR/Cas9 targeting Luciferase. This led to a slightly increased background of apoptosis even when IL-3 was available. However, although FOXO3A-TM is considered to be constitutively active, IL-3 deprivation was required to induce apoptosis (Fig. 6b, left). Importantly, apoptosis could be inhibited by addition of CT98014 (Fig. 6b, right), showing that FOXO3A-TM (which is not controlled by AKT) requires GSK-3 activity to induce apoptosis upon IL-3 withdrawal. As the inhibition of GSK-3 did not affect the binding to 14-3-3 proteins (Fig. 6c) or the nuclear localization of FOXO3A (Fig. S5A), the regulation of FOXO3A-mediated transcription by GSK-3 is independent of FOXO3A translocation. Interestingly, we observed an interaction of GSK-3 with FLAG-tagged FOXO3A overexpressed in 293T HEK cells (Fig. 6c). Together, the results show that activity of GSK-3 is decisive for the FOXO3A and p53-mediated PUMA induction in the absence of PI3K signaling.

Discussion
We had previously shown that GSK-3 phosphorylates the anti-apoptotic protein MCL-1 upon growth factor withdrawal or PI3K inhibition, which regulates lymphocyte survival 9,15 . In this study, we aimed at systematically identifying the GSK-3-dependent pro-apoptotic factors, promoting apoptosis upon depletion from growth factor. We showed that PUMA rather than BIM mediates IL-3 withdrawal-induced apoptosis, although both proteins were upregulated upon IL-3 deprivation (data not shown). In line with our data, it was previously shown that PUMA and BIM are induced by IL-2 withdrawal 5,16 . Our findings also confirm a previous report that IL-3-dependent myeloid cell lines from mice lacking PUMA, but not BIM, were protected from IL-3 withdrawal-induced apoptosis 17 .
FOXO3A and p53 were both described to be important for growth factor withdrawal-induced apoptosis. FOXO3A deficiency can protect cells from cytokine withdrawal-induced apoptosis 16,18 , and FOXO3A was shown to be crucial for PUMA induction upon growth factor withdrawal 5 . It was also found that p53, FOXO and  E-box-binding transcription factors share many targeted genes in response to PI3K inhibition in Rat-1 cells and a dominant-negative p53 resulted in partial resistance to apoptosis upon PI3K inhibition 19 . Likewise, HoxB8transformed factor-dependent myeloid (FDM) cells from p53 −/− mice were less sensitive for IL-3 deprivationinduced cell death and showed reduced PUMA induction. In this study, FDM cells from FOXO3A −/− mice showed an even higher susceptibility to cell death after IL-3 loss 20 . The same authors reported that PUMA induction was independent of PI3K, a finding which is not confirmed by our data 17,21 . Instead, we found that inhibition of the PI3K/AKT pathway upregulated PUMA in p53 −/− HCT116 cells and Ba/F3 cells.
In addition, p53 negative Ba/F3 cells were fully protected from apoptosis only when GSK-3 was inhibited, which further supports the important apoptosisregulatory role of the PI3K/AKT pathway also in absence of p53.
We have shown that the induction of Puma by FOXO3A and/or p53 is dependent on GSK-3 activity. Importantly, we observed that even the transcriptional activity of a FOXO3A-TM mutant, which is considered constitutively active due to the loss of the AKT phosphorylation sites, required GSK-3. Our results suggest that the inhibition of GSK-3 is the key pro-survival function of PI3K signaling, being more important than the inactivation of FOXO3A. In line with this finding, full transcriptional activity of Foxo1/3/4 was shown to require GSK-3, as the induction of IGF-IR gene by serum starvation or AKT inhibition required both active GSK-3 and Foxo1/3/4 8 . Supporting a PI3K-dependent regulation of Error bars represent SD from technical replicates. This experiment was done together with the one shown in Fig. 5b, and the controls are identical. c HEK 293T cell were transfected with constructs encoding wild-type, FLAG-tagged FOXO3A (wt) or FLAG-tagged FOXO3A-TM (TM) or control vector (−). Eight hours later, the cells were treated with CT98014 (0.75 µM) or left untreated for 18 h. The cells were lysed and 2% of the lysate were kept as input. FLAG immunoprecipitation was performed with the remaining lysate. After washing and elution with 3xFLAG peptide, eluate and input fractions were analyzed by western blotting with the antibodies indicated.
FOXO3A independent of the AKT phosphorylation sites, the DNA-binding and transcriptional activity of a triple S/ A mutant of the Foxo homolog in C. elegans, DAF-16, which is considered constitutive active, was shown to be further increased by PI3K inhibition by LY294002 and Wortmannin 22 . Interestingly, promotion of FOXO3A activity to induce PUMA by GSK-3 was independent of the interaction of FOXO3A with 14-3-3 or its subcellular localization. It is not clear however, how GSK-3 and FOXO3A cooperate. In a study of Terragni et al., upregulated genes upon inhibition of PI3K were co-regulated by FOXO3A, MITF, and USF1, with the latter two being regulated by GSK-3 23 . Another explanation for the functional interaction of FOXO3A and GSK-3 could be provided by the regulation of TIP60. We could previously demonstrate the requirement of GSK-3 for Puma induction by p53 in the context of DNA damage and subsequent p53 stabilization by the phosphorylation of the histone acetyl transferase Tip60 (KAT5). Increased KAT activity of Tip60 is induced by GSK-3 mediated phosphorylation, resulting in the acetylation of p53 in the DNA-binding domain at lysine 120, which enables p53 to promote the transcription of Puma. It is possible that GSK-3 activated Tip60 acts on chromatin or FOXO3A directly, thereby promoting transcriptional Puma induction 6 .
A direct phosphorylation of FOXO3A by GSK-3 would also be a possibility as to how GSK-3 directly promotes the transcriptional activity of FOXO3A, which would be consistent with our data demonstrating a cooperation of FOXO3A-TM and GSK-3 for the regulation of the Puma promoter.
Together, in this study, we provide evidence that Puma induction by FOXO3A is an important step in IL-3 withdrawal-induced apoptosis, and that the Puma inducing function of FOXO3A is dependent on GSK-3 activity. Thus, GSK-3 is crucial for the full activation of FOXO3A transcriptional activity when the PI3K pathway is not active. As pharmacological modulation of kinase signaling pathways is a promising strategy for cancer therapy, a more detailed understanding of the underlying mechanisms will improve defining the crucial targets. Generation of ko cell lines using lentiviral CRISPR/Cas9

Reagents and antibodies
The lentiCRISPRV2 system was used to generate ko cells as described by others 24 . At least two different guide RNAs for each target were designed using crispr.mit.edu and a guide RNA targeting Luciferase was designed as control (see Table 1: gRNAs). The guide RNAs were cloned into the lentiCRISPRV2 plasmid (a gift from Feng Zhang, Addgene plasmid # 52961) and lentiviral particles were produced by transfection of 5 μg lentiCRISPRv2, 1.5 μg pMISSIONVSV-G (Sigma-Aldrich) and 3 μg pMIS-SION GAG POL (Sigma-Aldrich) with Attractene (Quiagen, Hilden, Germany) into 293T HEK cells seeded at 25% confluency in a 78-cm² culture plate the day before transfection. The morning after transfection, fresh medium was added. In the evening, 4 ml medium was added. The next day, viral supernatants were harvested, filtered (0,45 μM) and supplemented with 5 μg/ml polybrene (Sigma-Aldrich). The target cells were infected by spinfection at 400×g for 10 min. The following day, selection was started using 4 μg/ml puromycin (Sigma-Aldrich) for at least 3 days and until no viable cells were detected in an uninfected control. Mix cultures were tested for Cas9 cleavage by performing a Surveyor assay. The region of interest was amplified by PCR using the primers listed ( Cell culture and treatment HCT116 p53 −/− , HCT116 p53 +/+ (kindly provided by Bert Vogelstein) and HCT116 (ATCC) were maintained in DMEM containing 10% FCS and Penicillin/Streptomycin (P/S). FL5.12 and BaF3 were maintained in RPMI-1640 with 10% FCS, P/S and 1 μg/l recombinant IL-3. To generate IL-2-dependent lymphoid cells, lymph node cells from three C57/Bl6 mice were isolated and activated by 20 ng/ml PMA and 0.5 μg/ml ionomycin for 48 h in RPMI-1640 with 10% FSC and P/S. The cells were then cultured in the medium containing 100 U/ml recombinant IL-2 for 24 h and then used for experiments. For growth factor withdrawal, cells were washed twice with 50 ml PBS and resuspended in RPMI-1640 with 10% FCS and P/S.

Western blotting
Cells were harvested and washed with ice-cold PBS. Cells pellets were lysed for 5 min on ice with lysis buffer (20 mM Tris-HCl pH 7.5, 150 mM NaCl, 1% Triton X-100, 1× protease inhibitor cocktail (Roche), phosphatase inhibitor cocktail 1 (1:50, Sigma-Aldrich), MG132 (20 μM, Alexis Biochemicals)) or by nuclear fractionation as described 6 . Lysates were cleared by centrifugation at 16.100×g for 10 min, 4°C. Protein concentration was determined using Bradford reagent (Bio-Rad). Lämmli buffer was added to 10-100 μg of protein lysate and samples were boiled at 95°C for 5 min. In some cases, the same amount of lysate was loaded on a second gel to probe for several antibodies. Proteins were separated by SDS-PAGE and transferred to nitrocellulose membranes. The membranes were blocked in 3% dry milk in TBS-Tween20 (0.1%) (TBS-T) and then incubated at 4°C overnight with the primary antibody diluted in 3% dry milk in TBS-T. The membranes were washed three times with TBS-T, incubated for 1 h with horseradish peroxidase (HRP)-conjugated secondary antibody at room temperature and washed three times with TSB-T. Band visualization was achieved using Super Signal West Pico Chemiluminescent Substrate (Thermo Scientific, Waltham, USA) and the Fusion Solo imaging system (Vilber Lourmat, Eberhardzell, Germany).

Luciferase reporter assay
293T HEK cells in 24-well plates were transfected with a total amount of 1 μg DNA by Attractene. A renilla luciferase expression plasmid (Promega, Madison, WI, USA) as internal control, a luciferase reporter plasmid driven by a 1.7 kb Puma promotor region (−1200 to +500 from
Overexpression of FOXO3A in HCT116 p53 −/− HCT116 p53 −/− in six-well plates were transfected by Lipofectamine® 2000 with pECE-FLAG-FOXO3A or empty vector according to the manufacturer's protocol. Twenty-four hours after transfection, the cells were treated with GDC-0941 (10 μM) for 5 h and then subjected to western blotting.

Statistical analysis
All data are shown as mean ± S.D. in case of technical replicates and as mean ± 95% confidence interval in case of biological replicates. The GraphPad Prism 5 software (GraphPad Software Inc., La Jolla, CA, USA) was applied for statistical analysis using the one-way ANOVA with post hoc Tukey's multiple comparison test. P ≤ 0.05 was regarded as the threshold value for statistical significance. *P ≤ 0.05; **P ≤ 0.01; ***P ≤ 0.001.