Fig. 5: Effects of regulating autophagy activity on CGs distribution of aging mouse oocytes. | Cell Death & Disease

Fig. 5: Effects of regulating autophagy activity on CGs distribution of aging mouse oocytes.

From: Role of autophagy in modulating post-maturation aging of mouse oocytes

Fig. 5

Oocytes that had aged for 12 h in FCM or FCM containing 10 nM rapamycin, 10 mM LiCl or 5 mM 3-MA were cultured for 24 h in CZB medium before examination for distribution of CGs. In the confocal images, DNA and CGs were pseudo-colored blue and green, respectively. Bar is 15 µm and applies to all images. Images a, b and c show oocytes with normal distribution (ND), early migration (EM) and late migration (LM) of CGs, respectively. Graph d shows percentages of oocytes with different patterns of CGs distribution following oocyte aging in different aging media. Each treatment was repeated three times with each replicate containing about 20 oocytes. ac Values with different letters above bars differ significantly (P < 0.05) within patterns of CGs distribution

Back to article page