Fig. 1: Activation rates and levels of LC3, P62 and active caspase-3 after mouse DOs were aged for different times in different media. | Cell Death & Disease

Fig. 1: Activation rates and levels of LC3, P62 and active caspase-3 after mouse DOs were aged for different times in different media.

From: Role of autophagy in modulating post-maturation aging of mouse oocytes

Fig. 1

Graphs a and d show percentages of activated oocytes. While a shows freshly collected control (Ctrl) oocytes and oocytes aging for 12 h in CZB + MG132 (C + M), CZB or FCM, d shows oocytes aging for different times in FCM. Each treatment was repeated 3–4 times with each replicate containing about 30 oocytes. Graphs b and c show LC3-II quantification by western blotting in oocytes aging for 12 h in different media. While b shows LC3-II contents (LC3-II/tubulin ratio), c shows the LC3-II/LC3-I ratio. Each treatment was repeated three times with each replicate containing about 200–250 oocytes. Graph e shows relative levels of active caspase-3 in oocytes aging in FCM for different times as detected by western blotting. Each treatment was repeated three times with each replicate containing about 300-400 oocytes. Graphs f, g and h show LC3-II contents (LC3-II/GAPDH ratio), LC3-II/LC3-I ratio and p62 (p62/GAPDH ratio), respectively, in oocytes aging in FCM for different times. Each treatment was repeated three times with each replicate containing about 200–250 oocytes. ad Values with a different letter above bars differ significantly (P < 0.05). Micrographs i, j and k are confocal images showing autophagosome (LC3-II puncta) distribution in oocytes aging in FCM for 0, 12 and 18 h, respectively. These are the merged pictures with chromosomes and LC3-II puncta pseudo colored blue and red, respectively. The bar is 15 µm and applies to all images

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