Fig. 4: HUCMSC-EVs increase the viability and proliferation of the LUAD cells and decrease their apoptosis. | Cell Death & Disease

Fig. 4: HUCMSC-EVs increase the viability and proliferation of the LUAD cells and decrease their apoptosis.

From: Human umbilical cord mesenchymal stem cell-derived extracellular vesicles promote lung adenocarcinoma growth by transferring miR-410

Fig. 4

a H1299 or PC-9 cells in serum-free media were incubated with the indicated amounts of hUCMSC-EVs for 24 or 48 h, and the cell viability was determined using the CCK8 assay. b H1299 or PC-9 cells in serum-free medium were treated with 1.5 × 109 per mL of hUCMSC-EVs for 24 or 48 h, and the cell proliferation was measured using an EdU cell proliferation assay. Representative contour plots of the EdU-positive cells are shown. c The percentages of EdU-positive cancer cells are shown. d H1299 or PC-9 cells were cultured in serum-free conditions and treated with various amounts of hUCMSC-EVs for 24 (H1299 cells) or 48 h (PC-9 cells). The apoptotic cells were determined by staining with 7-aminoactinomycin D (7-AAD) and Annexin-V after the treatment. Representative contour plots of the cell apoptosis are shown. The proportions of dead cells (Annexin V/7-ADD+), live cells (Annexin V/7-ADD), early apoptotic cells (Annexin V+/7-ADD), and late apoptotic/necrotic cells (Annexin V+/7-ADD+) were compared. e The percentages are shown for both early and late apoptotic cells. The data are expressed as the means ± SD of at least three replicates and *P < 0.05; **P < 0.01; ***P < 0.001, versus control (one-way ANOVA)

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