Germ cell differentiation during the epithelial cycle of spermatogenesis is accompanied by extensive remodeling at the Sertoli cell–cell and Sertoli cell–spermatid interface to accommodate the transport of preleptotene spermatocytes and developing spermatids across the blood–testis barrier (BTB) and the adluminal compartment of the seminiferous epithelium, respectively. The unique cell junction in the testis is the actin-rich ectoplasmic specialization (ES) designated basal ES at the Sertoli cell–cell interface, and the apical ES at the Sertoli–spermatid interface. Since ES dynamics (i.e., disassembly, reassembly and stabilization) are supported by actin microfilaments, which rapidly converts between their bundled and unbundled/branched configuration to confer plasticity to the ES, it is logical to speculate that actin nucleation proteins play a crucial role to ES dynamics. Herein, we reported findings that Spire 1, an actin nucleator known to polymerize actins into long stretches of linear microfilaments in cells, is an important regulator of ES dynamics. Its knockdown by RNAi in Sertoli cells cultured in vitro was found to impede the Sertoli cell tight junction (TJ)-permeability barrier through changes in the organization of F-actin across Sertoli cell cytosol. Unexpectedly, Spire 1 knockdown also perturbed microtubule (MT) organization in Sertoli cells cultured in vitro. Biochemical studies using cultured Sertoli cells and specific F-actin vs. MT polymerization assays supported the notion that a transient loss of Spire 1 by RNAi disrupted Sertoli cell actin and MT polymerization and bundling activities. These findings in vitro were reproduced in studies in vivo by RNAi using Spire 1-specific siRNA duplexes to transfect testes with Polyplus in vivo-jetPEI as a transfection medium with high transfection efficiency. Spire 1 knockdown in the testis led to gross disruption of F-actin and MT organization across the seminiferous epithelium, thereby impeding the transport of spermatids and phagosomes across the epithelium and perturbing spermatogenesis. In summary, Spire 1 is an ES regulator to support germ cell development during spermatogenesis.
In actively migrating mammalian cells such as macrophages and fibroblasts, they generate branched (i.e., unbundled) actin filament networks and parallel actin filament bundles in lamellipodia and filopodia, respectively, by engaging two entirely different actin polymerization machineries: the Arp2/3 complex and the Spir/formin actin nucleator complex to support cell movement1,2,3,4,5. During spermatogenesis, developing germ cells, in particular post-meiotic spermatids that are nonmotile cells per se, must be transported across the entire seminiferous epithelium during spermiogenesis so that fully developed spermatids (i.e., spermatozoa) can line-up at the luminal edge of the apical compartment to prepare for their release at spermiation at stage VIII of the epithelial cycle6,7,8,9. While Sertoli cells are motile cells when cultured in vitro, they no longer actively migrate around the seminiferous epithelium but serve as the ‘nurse’ cells by nurturing germ cells to support their development. Furthermore, neither Sertoli nor germ cells possess lamellipodia and filopodia in vivo to support active cell movement. Instead, germ cells rely on the Sertoli cells in particular the actin- and microtubule (MT)-based cytoskeletons in Sertoli cells to provide the support and machineries so that they can be transported across the seminiferous epithelium during the epithelial cycle10,11,12,13. Studies have shown that the testis-specific adherens junction (AJ) known as ectoplasmic specialization (ES) that are found at the Sertoli–spermatid (step 9–18) interface (i.e., apical ES) is the only anchoring junction that supports spermatid transport during spermiogenesis; and ES is also found at the Sertoli cell-cell interface (i.e., basal ES), which is the crucial component of the blood–testis barrier (BTB) that supports preleptotene spermatocyte transport across the immunological barrier7,8,14,15,16. Since the ES in the testis is constituted and supported by an array of actin microfilament bundles and an adjacent network of MTs, it is generally accepted that the actin- and MT-based cytoskeletons in Sertoli cells play a crucial role to support germ cell transport during spermatogenesis8,10,12,14,17,18. Indeed, studies have shown that Sertoli cells in the testis are utilizing the Arp2/3 (actin related protein 2/3)-N-WASP (neural Wiskott-Aldrich syndrome protein) complex19 and formin 120,21 to regulate F-actin organization at the apical and basal ES to support germ cell transport in the epithelium during the epithelial cycle. However, it remains to be investigated if Spire is expressed by Sertoli and/or germ cells and if it is involved in regulating F-actin organization in the testis.
Similar to formins (e.g., formin 120,21,22), Spire such as Spire 1 and Spire 2 is a WH2 (WASP-homology 2, an actin monomer-binding motif consisting of ~ 17 amino-acid residues) domain-containing actin nucleator4,23. But, unlike formins such as formin 1 which functions as a dimerized protein, Spire is a monomeric protein capable of inducing actin polymerization via the addition of ATP-actin monomers to the filament ‘barbed end’22. Spire has four WH2 domains in tandem located in the center of its polypeptide sequences to recruit ATP-actin monomers to initiate actin polymerization, thus it is capable of generating long stretches of linear actin microfilaments efficiently4,23. These actin filaments can then be bundled via the action of actin bundling proteins Eps824, palladin25, and plastin 326 to support the actin microfilament bundles at the ES. While Spire functions as an independent actin nucleator as a monomeric protein, Spire can also dimerize when it is binding to formins, creating the formin/Spire nucleator complex to induce efficient actin filament polymerization, generating long stretches of linear actin microfilaments in mammalian cells4,23. Thus, in order to better understand the biology of actin polymerization that maintains actin organization at the ES, we sought to investigate the role of Spire 1 in the rat testis as reported herein.
Materials and methods
Animals and ethics statement
Adult male Sprague-Dawley rats (280–300 g body weight) used for various in vivo experiments, or male pups to be used at 20 days of age for the isolation of Sertoli cells for primary cultures, were purchased from Charles River Laboratories (Kingston, NY). Rats were housed at the Rockefeller University Comparative Bioscience Center (CBC) in accordance with the applicable portions of the Animal Welfare Act and the guidelines in the Department of Health and Human Services publication Guide for the Care and Use of Laboratory Animals. The use of animals was approved by the Rockefeller University Institutional Animal Care and Use Committee (IACUC) (Protocol Number 12–506 and 15–780-H). The use of siRNA duplexes for applicable in vitro and in vivo experiments was approved by Rockefeller University Institutional Biosafety Committee (IBC) (Approval Number 2-15-04-007). All rats were euthanized by CO2 asphyxiation using slow (20 ~ 30%/min) displacement of chamber air with compressed carbon dioxide in an euthanasia chamber approved by the Rockefeller University Laboratory Safety and Environmental Health (LSEH).
Antibodies used for various experiments reported here were obtained commercially from different vendors except otherwise specified. The RRID (Resource Identification Initiative) numbers of all antibodies are included in Table 1, as well as their working dilutions used for various experiments.
Isolation and primary culture of Sertoli cells
Sertoli cells were isolated using 20-day-old rat testes as described27. Freshly isolated Sertoli cells were seeded on Matrigel- (BD Biosciences, San Jose, CA)-coated culture dishes (either 6, 12- or 24-wells), coverslips (placed in 12-well dishes) and bicameral units (Millipore, Billerica, MA) (placed in 24-well dishes) at a density 0.4–0.5, 0.03–0.04, and 1.0 × 106 cells/cm2 respectively, in serum-free DMEM/F-12 (Gibco) medium, supplemented with growth factors and gentamicin in a humidified atmosphere of 95% air/5% CO2 (vol/vol) at 35 °C27. For 6-well and 12-well dishes, each well contained 5 or 3 ml F12/DMEM medium for specific biochemical assays or for immunoblottings. But for IF, each well (with coverslip) contained 2 ml F12/DMEM. For bicameral units placed in 24-well dishes, the apical and the basal chamber contained 0.5 ml F12/DMEM. All media were supplemented with growth factors and gentamicin. On day 2 (about 36 h after cell plating), residual germ cells were removed by a brief hypotonic treatment (20 mM Tris, pH 7.4 at 22 °C, 2.5 min) as described28 so that these cultures with ~98% pure with some cellular debris and without detectable contaminations of either germ cells, Leydig cells and/or peritubular myoid cells using corresponding primer pairs and/or antibodies against specific marker proteins for RT-PCR or immunoblottings as described29. These Sertoli cell cultures were used for experiments on day 3 with an established functional tight junction (TJ)-permeability barrier, and ultrastructures of TJ, basal ES, gap junction, and desmosome that mimicked the Sertoli cell blood–testis barrier (BTB) in vivo were also detected as earlier described30,31,32.
Knockdown (KD) of Spire 1 by RNA interference (RNAi) in Sertoli cells in vitro
Spire 1 was silenced in Sertoli cells by RNAi to assess the effects of its KD on Sertoli cell function. In brief, Sertoli cells cultured alone on day 3 with an established functional TJ-permeability barrier were transfected with Spire 1-specific small interfering RNA (siRNA) duplexes vs. non-targeting negative control (Dharmacon) duplexes at 100–150 nM (for IB and actin polymerization/bundling assay) or 50–100 nM (for IF) using RNAiMAX (Life Technologies) as a transfection reagent for 24 h as earlier described26 (Table 2). Thereafter, cells were washed thrice to remove transfection reagents, replaced with fresh F12/DMEM and cultured for an additional 24 h before RNA was extracted for analysis by RT-PCR or qPCR (day 5). For IF, IB or actin polymerization/bundling assay, cells were cultured until day 6 before termination. For cultures to be used for IF, cells were co-transfected with 1 nM siGLO red transfection indicator (Dharmacon) to track successful transfection. In each experiment, replicates or triplicates were used for each treatment vs. control groups, and each experiment was repeated with n = 3 using different batches of Sertoli cells.
Knockdown of Spire 1 in adult rat testes in vivo
Spire 1 was silenced in adult rat (~280–300 g body weight) testes in vivo by transfecting testes with Spire 1 siRNA duplexes vs. non-targeting control using Polyplus in vivo-jetPEI (Polyplus-transfection S.A., Illkirch, France) as a transfection reagent according to manufacturer’s instructions as earlier described33,34, which was shown to have a considerable improvement in transfection efficiency (at ~ 70%) when compared to conventional transfection medium (at ~ 30%)34,35,36. In brief, siRNA duplexes (500 nM) and siGLO red transfection indicator (Dharmacon) (40 nM) were constituted in 100 µl of transfection solution containing 1.7 µl in vivo-jetPEI (an adult rat testis was at ~1.6 g in weight, with a volume of ~ 1.6 ml) according to manufacturer’s recommendations with N/P = 8 (note: N/P ratio refers to number of nitrogen (N) residues of jetPEI/nucleotide phosphate (P), wherein the jetPEI concentration is expressed in nitrogen residues molarity and 1 µg of plasmid DNA contains 3 nmol of anionic phosphate; i.e., 500 nM (11.09 µg) siRNA required 1.7 µl in vivo-jetPEI), and administered to each testis using a 28-gauge 13 mm needle attached to a 0.5 ml insulin syringe. The needle was inserted from the apical to the basal end of the testis vertically in which the right testis was transfected with the Spire 1 siRNA duplexes vs. the left testes transfected with the negative non-targeting control siRNA duplexes. As the needle was withdrawn apically, transfection solution was released and gradually filled the entire testis to avoid an acute rise in intra-testicular hydrostatic pressure. Transfection was performed on day 1, 2 and 3 (triple transfections, n = 2 rats) and in some experiments, transfection was performed on day 1, 3 and 5 (triple transfections, n = 3 rats). Rats were euthanized on day 5 (n = 2 rats) or day 7 (n = 3 rats), respectively. Testes were removed immediately, frozen in liquid nitrogen or fixed in Bouin’s fluid or modified Davidson’s fixative37,38 for their subsequent use. Since the phenotypes in these two groups of rats were similar, data from both sets of experiments were pooled for analysis with n = 5 rats.
Assessment of Sertoli cell TJ-permeability barrier in vitro
Sertoli cells cultured in vitro assembled an intact epithelium with a functional TJ-barrier, capable of resisting the conductivity of electrical current delivered by a Millipore Millicell-ERS (electrical resistance system) meter that was sent across the barrier. This resistance was then recorded by quantifying the transepithelial electrical resistance (TER) in ohms (Ω) by placing two electrodes across the Sertoli cell epithelium, one in the apical and one in the basal compartment of the bicameral units. In brief, Sertoli cells were plated on Matrigel-coated Bicameral units (diameter 12 mm; pore size 0.45 µm, effective surface area 0.6 cm2; EMD Millipore) at 1.0 × 106 cells/cm2. Each bicameral units was placed inside the well of a 24-well dish with 0.5 ml F12/DMEM each in the apical and the basal compartments. Spire 1 was KD in Sertoli cells cultured alone on day 3 using specific siRNA vs. non-targeting negative control duplexes at 150 nM for 16 h, and Sertoli cell TJ-permeability barrier function was monitored daily by quantifying TER across the cell epithelium as described27. In each experiment, each treatment and the control group had triplicate or quadruple bicameral units. Each experiment was done with n = 3 using different batches of Sertoli cells, excluding pilot experiments to assess the optimal concentrations of siRNA duplexes.
BTB integrity assay in vivo
The Sertoli cell BTB integrity in vivo was assessed by the ability of the Sertoli cell TJ/basal ES-barrier to block the diffusion of a membrane impermeable biotinylation reagent EZ-Link Sulfo-NHS-LC-biotin (Thermo Fisher Scientific, Waltham, MA) across the barrier as earlier described35. In brief, rats under anesthesia using ketamine HCl (60 mg kg b.w., i.m.) with xylazine as an analgesic (10 mg/kg b.w., i.m.) received 100 µl of 10 mg/ml EZ-Link Sulfo-NHS-LC-biotin by loading the biotin under the tunica albuginea with a 29-gauge needle of n = 3 rats for each group including control groups. About 30 min thereafter, rats were euthanized by CO2 asphyxiation, testes were immediately removed, freezed in liquid nitrogen. Thereafter, frozen 10 µm-thick cross-sections were obtained in a cryostat at −22 °C, fixed in 4% PFA in PBS for 10 min and incubated with Alexa Fluor 488-streptavidin (1:250) for 30 min. Microscopic slides were mounted with Prolong Gold Antifade reagent with DAPI (Life Technologies). Testes from normal (untreated) rats served as negative control and rats received 3 mg/kg b.w. cadmium chloride (i.p.) (for 3 days) served as a positive control which is known to induce irreversible BTB damage39. Semi-quantitative data to assess the BTB integrity were obtained by quantifying the distance (D) of the biotin (DBiotin) traversed from the basement membrane vs. the radius of a seminiferous tubule (DRadius). For oblique sections of the tubules, DRadius was obtained using the average of the shortest and the longest distance from the basement membrane. About 50 randomly selected tubules from each testis of n = 3 rats with a total of 150 tubules were examined (including the control groups).
RNA extraction, qPCR and RT-PCR
Total RNA was isolated from rat testes, Sertoli cells, germ cells and brain using Trizol reagent (Life Technologies) as described26,40. 2 µg total RNA was reverse transcribed by Moloney murine leukemia virus reverse transcriptase (M-MLV) (Promega, Madison, WI) according to manufacturer’s instructions to obtain cDNAs which served as templates for subsequent PCR. To quantify Spire 1 or Spire 2 steady-state mRNA level, PCR was performed using cDNA products obtained above as templates with a primer pair specific for Spire 1 or Spire 2 vs. S16 (Table 3). Authenticity of PCR products was verified by gel electrophoresis, which was then extracted for direct DNA sequencing at Genewiz (South Plain field, NJ). Each RT-PCR experiment was performed with n = 3 using different batches of Sertoli cells or germ cells vs. rat testis and brain from 3 different male rats. qPCR was performed as described34, the mRNA level of Spire 1 and Spire 2 was analyzed by QuantStudioTM 12 K Flex Real-Time PCR System (Thermo Fisher, Waltham, MA) with Power SYBR Green Master Mix (Applied Biosystems, Foster City, CA) according to the manufacturer’s instructions (n = 3). Gapdh was used as an internal control for normalization. The specificity of the fluorescence signal was verified by both melting curve analysis and gel electrophoresis. The expression level of the target gene was determined using 2-ΔΔCT method.
Protein lysate preparation, protein estimation and immunoblot (IB) analysis
Cells or testes were lysed in immunoprecipitation (IP) lysis buffer (50 mM Tris, containing 0.15 M NaCl, 1% Nonidet P-40 (vol/vol), 1 mM EGTA, 10% glycerol (vol/vol), pH 7.4 at 22 °C, freshly supplemented with protease inhibitor mixture (Sigma-Aldrich) and phosphatase inhibitor cocktail II (Sigma-Aldrich)), to be followed by an 8-s sonication (2 × , with 1 min interval in ice) using a Cole-Parmer Ultrasonic Processor (Model CPX130PB, Cole-Parmer, Chicago, IL) to obtain protein lysates by placing microfuge tubes on ice. For testes samples, we routinely used a sample:IP lysis buffer of 1:5. After centrifugation at 16,000 × g for 1 h at 4 °C, clear supernatant was used for subsequent experiments. Protein estimation was performed using a Bio-Rad DC protein assay kit (Bio-Rad Laboratories, Hercules, CA) with BSA as a standard. Immunoblot analysis was performed as described34 using equal amount of total protein lysate between samples in each experiment: 20 or 60 μg protein for cell or testis lysates, respectively, using corresponding antibodies for different marker proteins (Table 1). Chemiluminescence was performed using an in-house prepared kit as described41, and signals were detected using an ImageQuant LAS 4000 (GE Healthcare Life Sciences) Imaging system and ImageQuant software (Version 1.3). GAPDH and β-actin served as a protein loading control. Protein band intensities were evaluated by ImageJ 1.45s which was obtained at http://rsbweb.nih.gov/ij (National Institutes of Health (NIH), Bethesda, MD). All samples within an experimental group were processed simultaneously to avoid inter-experimental variations. Each sample had triplcates in both silencing and control groups with n = 3 independent experiments using different batches of Sertoli cells or testes from three different rats.
Immunofluorescence analysis (IF), F-actin staining and fluorescence image analysis
IF was performed using frozen cross sections of testes at 7 μm obtained in a cryostat at −22 °C, or fresh Sertoli cells cultured on coverslips as earlier described26,42. Sections or cells were fixed in 4% paraformaldehyde (PFA) or ice-cold methanol for 10 min (min), permeabilized in 0.1% Triton X-100 for approximately 5–10 min, blocked in 10% goat serum (v/v) or 5% BSA (w/v) in PBS (10 mM sodium phosphate, 0.15 M NaCl. pH 7.4 at 22 °C). Thereafter, samples were incubated with a specific primary and the corresponding secondary antibodies (Table 1), and co-stained with 4′,6-diamidino-2-phenylindole (DAPI) (Sigma) to visualize cell nuclei. Slides were mounted in Prolong Gold Antifade reagent (Invitrogen, Life Technologies). For F-actin staining, testis sections or Sertoli cells were incubated with Alexa Fluor 488 phalloidin (Invitrogen) according to manufacturer’s instructions. Images were examined and acquired using a Nikon Eclipse 90i Fluorescence Microscope system equipped with Nikon Ds-Qi1Mc or DsFi1 digital camera and Nikon NIS Elements AR 3.2 software (Nikon, Tokyo, Japan) and saved in TIFF format. Image overlays were performed using Adobe Photoshop CS4 (San Jose, CA). Fluorescence intensity was analyzed by ImageJ 1.45 s (NIH, Bethesda, MD) or Nikon NIS Elements AR (Version 3.2) software package. Sections or cells in an experiment including both control and treatment groups were analyzed in a single experimental session to eliminate inter-experimental variations. Data shown were representative micrographs from a single experiment, and each experiment had three coverslips of cells or different cross-sections of testes. Each experiment was repeated at least three times using different batches of Sertoli cells or testes from 3 rats which yielded similar results. For fluorescence intensity or distribution analysis in Sertoli cells or seminiferous tubules of testes, at least 200 cells or 200 cross-sections of tubules were randomly selected and examined in both experimental and control groups in an experiment, and a total n = 3 experiments were performed.
Assessing the ratio of F (filamentous) actin to G (globular) actin by a spin-down assay
The relative ratio of F:G actin in Sertoli cell or testis lysates following Spire 1 silencing was assessed according to the manufacturer’s instructions (Cat No. BK037, Cytoskeleton, Denver, CO) with minor modifications. In brief, Sertoli cells (~ 500 µg protein from a single well of a 6-well dish at 0.4 × 106 cells/cm2) or testes (~ 1 mg) were homogenized in F-actin stabilization buffer, pre-cleared by centrifugation at 350 g for 5 min at room temperature to remove cell debris, followed by centrifugation at 100,000 g at 37 °C for 1 h to separate F-actin from G-actin. Supernatant (~2 ml, containing G-actin) was collected, pellet (containing F-actin) was re-suspended in 300 µl 8 M urea. Thereafter, 60 µl of supernatant and 60 µl of pellet of each sample were analyzed by immunoblotting for β-actin. Phalloidin (0.1 µM, actin stabilizing agent) vs. urea (80 mM, actin depolymerization agent) was used as the corresponding positive and negative controls. This thus assessed any changes in the relative ratio of F-actin incorporated into the cytoskeleton vs. the G-actin pool in the cytosol in Sertoli cells (or testes) following Spire 1 knockdown when compared to testes in control group transfected with non-targeting negative control siRNA duplexes.
Actin bundling assay
Actin bundling assay was performed as earlier described20,26 to assess the ability of Sertoli cell lysates to bundle premade F-actin (purified and polymerized F-actin from human platelets) in vitro following Spire 1 silencing compared to the corresponding controls according to the manufacturer’s instructions (Cat No. BK013, Cytoskeleton). In brief, Sertoli cell lysates (obtained by centrifugation following cell or tissue lysis in a Tris-based lysis buffer20,26) in 10 µl (containing ~ 20–40 µg total protein) obtained from cells following Spire 1 kD vs. control cells were incubated with 40 µl of F-actin (at 21 µM) for 1 h at room temperature to allow bundling of F-actin, to be followed by centrifugation at 14,000 × g at 24 °C for 5 min to pellet bundled F-actin from the linear unbundled F-actin and G-actin in the supernatant. Pellet was dissolved in 30 µl sterile H2O and 2 µl supernatant of each sample were analyzed by immunoblotting using an anti-β-actin antibody. All samples, including both Spire 1 KD vs. controls were analyzed in a single experiment to avoid inter-experimental variations.
Actin polymerization assay
Actin polymerization assay was performed as described20,26 to assess the ability of Sertoli cell lysate following Spire 1 silencing vs. controls to polymerize pyrene actin oligomers in vitro according to manufacturer’s instructions (Cat No. BK003, Cytoskeleton). In brief, Sertoli cell lysate (obtained by centrifugation following cell or tissue lysis in a Tris-based lysis buffer20,26) in 30 µl (containing approximately 60–120 µg total protein) was incubated with 60 µl of pyrene actin oligomers at 0.4 mg/ml. Polymerization was initiated by adding 10 µl of a 10× actin polymerization buffer to each sample in a Corning 96-well black flat bottom polystyrene microplate (Corning, Lowell, MA, USA). Fluorescence kinetics were monitored from the top in a FilterMax F5 Multi-Mode Microplate Reader and the Multi-Mode Analysis Software 3.4 (Molecular Devices, Sunnyvale, CA) at room temperature (fluorimeter settings, measurement type: kinetic, 100 cycle, 20 s interval; excitation wavelength: 360 nm; emission wavelength: 430 nm; integration time: 0.25 ms). Actin polymerization rate assessed by fluorescence intensity increase rate was obtained by linear regression analysis using Microsoft Excel 2016 (Microsoft, WA). Phalloidin (1 µM, an actin stabilizing agent) vs. urea (100 mM, an actin depolymerization agent) recommended by the manufacturer was used as the corresponding positive and negative controls. For all the actin assays, the same amount of Sertoli cell lysate (40 µg total protein) or testis lysate (60 µg total protein) from each sample was also analyzed by immunoblot to confirm the knockdown of Spire 1. Each sample had replicates and each experiment was repeated at least 3 times (i.e., n = 3) using different batches of Sertoli cells or testes from n = 3 rats which yielded similar results.
Microtubule spin-down assay
MT spin-down assay was performed as earlier described21,43. This assay estimated the relative level of polymerized MTs vs. free tubulins in Sertoli cell cytosol according to the manufacturer’s protocols (Cat No. BK038, Cytoskeleton, Denver, CO). In brief, Sertoli cells were homogenized in 37 °C pre-warmed lysis and MT stabilization buffer (100 mM PIPES, 5 mM MgCl2, 1 mM EGTA, 0.1% NP-40, 0.1% Triton X-100, 0.1% Tween 20, 0.1% 2-mercaptoethanol, 30% glycerol, pH 6.9) with a 25-gauge syringe needle, pre-cleared by centrifugation at 2000 × g for 5 min at 37 °C to remove cellular debris, followed by centrifugation at 100,000 × g at 37 °C for 30 min to separate polymerized tubulins/MTs (pellet) from tubulin monomers (supernatant). The supernatant was collected, and the pellet was re-suspended in 250 µl 2 mM CaCl2. Cell lysates, pellet and supernatant were then used for IB using an anti-β-tubulin antibody. Paclitaxel (20 µM, also known as Taxol, a MT stabilizing agent) vs. CaCl2 (2 mM, a MT depolymerization agent) was used suggested by the manufacturer to serve as the corresponding positive and negative controls by including either paclitaxel or CaCl2 in the Sertoli cell lysate when the polymerized tubules/MT and free tubulin monomers were obtained as described above. In short, this assay assessed changes in the relative distribution of MTs/polymerized tubulins vs. free/non-polymerized tubulin monomers in Sertoli cell cytosol following Spire1 knockdown when compared to control cells transfected with non-targeting negative control siRNA duplexes.
Testes obtained from rats after euthanasia by carbon dioxide asphyxiation were immediately fixed in Bouin’s fixative (Polysciences) or modified Davidson’s fixative37,38, and then embedded in paraffin. Sections at 5 µm thickness were obtained with a microtome, mounted on microscopic slides and staining with hematoxylin and eosin after deparaffinization. For histological analysis, at least 5000 tubules in each sample (grouped as stages I–V, VI–VIII, XI–XIV) were randomly selected and examined using cross-sections of a testis for a treatment vs. control group, and representative findings reported herein were derived from separate experiments with n = 4 rats which yielded similar results. Analysis was focused on stage VI–VIII and XI–XIV tubules because defects in spermatid or phagosome transports were notably detected at these stages of the epithelial cycle.
In each experiment, the treatment and the control groups had 2–4 replicates or at least 4 rats (and for each time points wherever applicable), and all experiments were repeated at least 3 times using different batches of Sertoli cells or rats. Data analyses were performed using SPSS 16.0 (SPSS, IL). The bar and line graphs were plotted with OriginPro 8 (OriginLab, MA) or Excel 2016 (Microsoft, WA, USA). Data are presented as the mean ± s.d. of n = 3 to 5 experiments (or n = 4 rats). Data were subjected to homogeneity test for variance, analyzed by Student’s t-test (2-tailed), and P value < 0.05 was considered statistically significant.
Expression of Spire 1 in Sertoli and germ cells in rat testes and its distribution in the seminiferous epithelium during the epithelial cycle
A study by RT-PCR using a primer pair specific to Spire 1 (Table 3) has shown that the actin nucleation protein Spire 1, but not Spire 2, was expressed in Sertoli and germ cells in the testis (Fig. 1a). Using an antibody specific to Spire 1 (Fig. 1b), the expression of Spire 1 by Sertoli cells when cultured in vitro to assemble the tight junction (TJ)-permeability barrier (Fig. 1c) rose steadily but remained plateaued when the barrier was established by day approximately 2–3 (Fig. 1c). Expression of Spire 1 was detected across the Sertoli cell cytosol, partially co-localized with actin microfilaments (Fig. 1d). Spire 1 was expressed in the seminiferous epithelium, prominently associated with apical ES at the Sertoli-elongating/elongated spermatid interface such as in stages VI–VIII tubules and conspicuously found at the convex (dorsal) side of spermatid heads, co-localizing with F-actin; it also expressed in the basal compartment near the basal ES/BTB and partially co-localized with F-actin (Fig. 1e). As noted in stage VII tubules when Spire 1 expression was high (Fig. 1e), dual-labeled immunofluorescence analysis (IF) in higher magnified images (Fig. 2a) had shown that Spire 1 was prominently expressed at the convex side of spermatid heads, but some minor but consistent expression was also detected at the concave (ventral) side, co-localizing with Arp3 (a branched actin nucleation/polymerization protein known to convert linear actin microfilaments to a branched actin network) and Eps8 (an actin barbed end capping and bundling proteins) at the concave side of spermatid heads (Fig. 2a) where Arp3 and Eps8 were robustly expressed in stage VII tubules, consistent with earlier reports19,24. Also, Spire 1 co-localized with apical ES proteins β1-integrin (expressed by Sertoli cells at the apical ES44,45), nectin 2 (expressed by both Sertoli cells and spermatids46) and nectin 3 (expressed by elongating/elongated spermatids47 at the apical ES) since these three apical ES proteins all expressed prominently at the convex side of spermatid heads (Fig. 2b). At higher magnification, Spire 1 was prominently expressed at the basal ES/BTB, co-localizing with basal ES proteins N-cadherin and γ-catenin (Fig. 2c).
Spire 1 knockdown perturbs Sertoli cell TJ-permeability barrier through changes in the distribution of proteins at the Sertoli cell–cell interface in vitro
Figure 3a is the regimen used to obtain lysates for immunoblot analysis shown in Fig. 3b. When Spire 1 was knockdown by approximately 70–75% based on IB (Fig. 3b, c) or qPCR (Fig. 3c), the steady-state levels of all the BTB-associated proteins including hemidesmosome protein were unaffected, illustrating the Spire 1 knockdown had no apparent off-target effects (Fig. 3b). However, Spire 1 knockdown was found to perturb the Sertoli cell TJ-permeability barrier function (Fig. 3d). When Sertoli cells from this experiment were examined by IF on day 5, a knockdown of Spire 1 by ~ 75% (based on fluorescence intensity analysis) was found to perturb the distribution of both TJ- (e.g., CAR, ZO-1) and basal ES- (e.g., N-cadherin, ß-catenin) at the Sertoli cell–cell interface (Fig. 3e). These proteins were internalized, possibly via endocytosis, since they no longer tightly localized at the cell cortical zone, but diffusively localized, re-distributing into the cell cytosol (Fig. 3e, see also histograms on the right panel).
Knockdown of Spire 1 that perturbs TJ-barrier function is mediated by disruptive changes in Sertoli cell actin organization
Knockdown of actin nucleation protein Spire 1 by RNAi also found to perturb the organization of actin filaments across the Sertoli cell cytosol (Fig. 4a). For instance, in control Sertoli cells, actin filaments were stretched across the Sertoli cell cytosol in an organized fashion (Fig. 4a, top panel). However, considerably less actin filaments were found in Sertoli cell cytosol following Spire 1 knockdown; instead, actin filaments were retracted to be closer to cell nuclei (Fig. 4a). These changes were supported, at least in part, by changes in the distribution of other actin regulatory proteins. For instance, palladin (an actin bundling protein expressed by Sertoli cells25) no longer stretched across the Sertoli cell cytosol to support bundling of actin microfilaments to form the stress fibers following Spire 1 knockdown, but retracted to be closer to the cell nuclei (Fig. 4a). The distribution of formin 1, another actin nucleator, in Sertoli cells was also perturbed in which formin 1 retracted closer to the Sertoli cell nucleus. The distribution of Arp3 (branched actin nucleation protein) and Eps8 (actin barbed end capping and bundling protein) was also perturbed as these proteins no longer localized prominently at the Sertoli cell–cell interface to confer actin dynamics and/or plasticity but re-distributed into the cell cytosol, closer to the cell nuclei (Fig. 4a). Such disruptive changes in the distribution of these actin regulatory proteins thus impeded the organization of F-actin across the cell cytosol. Furthermore, following the knockdown of Spire 1, there were considerable reduction in the ratio of F:G ratio (Fig. 4b) and overall actin bundling activity (Fig. 4c). Furthermore, the kinetics and the rate of actin polymerization following Spire 1 knockdown were impeded (Fig. 4d).
Knockdown of Spire 1 in Sertoli cells perturbs the organization of microtubule (MT)-based cytoskeleton
Spire 1 knockdown in Sertoli cells cultured in vitro was also found to perturb the organization of MTs across the cell cytosol (Figure 5a). α-Tubulins (note: α- and β-tubulins are the building blocks of MTs12) that constituted MTs, which stretched across the entire Sertoli cell cytosol to confer typical spindle-shape to these cells were retracted from cell peripheries but wrapped around the cell nuclei following Spire 1 knockdown (Fig. 5a). Besides α-tubulin, detyrosinated-α-tubulin, which rendered MTs to become stabilized (i.e., less dynamic) wherein its C-terminal Tyr (Y) was removed by exposing Glu (E) at its newly formed C-terminus48,49,50, also no longer stretched across the Sertoli cell cytosol as in control Sertoli cells, but wrapped and entangled around the cell nuclei following Spire 1 knockdown (Fig. 5a). Similar phenotype was also noted for EB1 (end binding protein 1, a plus (+) end tracking protein (+TIP) that binds to the plus (+) fast growing end of MTs to stabilize MTs) in which EB1 (seen as dot-like structures that laid across the +-end of MTs and stretched across the entire Sertoli cell cytosol) retracted from cell cytosol and localized closer to the cell nuclei (Fig. 5a). These findings were supported by a biochemical-based MT spin-down assay which monitored the relative level of polymerized MTs in Sertoli cells since Spire 1 knockdown led to a considerable reduction in polymerized MTs in Sertoli cells (Fig. 5b).
Knockdown of Spire 1 in the testis perturbs spermatogenesis
In vivo knockdown of Spire 1 in the testis was performed using Spire 1-specific siRNA duplexes vs. control siRNA duplexes using Polyplus in vivo-jetPEI as a transfection medium on day 1, 2 and 3 (i.e., a total of 3 transfections) (n = 2 rats) and terminated on day 5; or on day 1, 3 and 5 (also 3 transfections) (n = 4 rats) and terminated on day 7 (Fig. 6a). The two testes from the same animal received either Spire 1 siRNA duplexes or the control siRNA duplexes. Since the phenotypes from both sets of animals were similar, data were pooled for analysis and shown in Fig. 6. A study by qPCR using specific Spire 1 and Spire 2 primer pairs (Table 3) showed that only Spire 1 was knockdown by ~70%, but the expression of Spire 2 was unaffected (Fig. 6b), illustrating the knockdown was specific to Spire 1. In control testes, only ~4% of tubules had signs of defects in spermatogenesis, but following 70% knockdown of Spire 1 in the testis, about 12% of tubules, representing a 3-fold increase vs. controls, had defects in spermatogenesis using the criteria outlined in Materials and Methods based on histological analysis (Fig. 6c). IB analysis demonstrated that specific downregulation of Spire 1 in the testis by ~70% (Fig. 6d), which failed to induce changes in any of the BTB-associated protein, had no apparent off-target effects (Fig. 6d). Defects in spermatogenesis following Spire 1 knockdown most notably confined to stage VIII–XII tubules in which the transport of elongated spermatids and other intracellular organelles (e.g., phagosomes) across the epithelium were grossly disrupted (Fig. 6e). The most obvious defects detected in stages VIII–XII tubules were: (i) the presence of multinucleated round spermatids, (ii) persistent presence of step 19 spermatids in stages VIII–XII tubules embedded deep inside the epithelium which should have been released into the tubule lumen at stage VIII, (iii) step 19 spermatids trapped in the epithelium had defects in polarity with their heads pointed at least 90–180° away from the basement membrane, and (iv) persistent presence of phagosomes in the adluminal compartment near the tubule lumen when they should have been transported to the base of the epithelium for lysosomal degradation (Fig. 6e). These phenotypes were likely the result of defects in actin- and/or MT-mediated transport of spermatids and organelles (e.g., phagosomes). These changes also perturbed intracellular trafficking, causing the generation of giant multinucleated round spermatids (Fig. 6e), which would eventually undergo degeneration and be degraded by Sertoli cells. Furthermore, some round and multinucleated round spermatids were detected in the epididymis, not found in the control epididymis (Fig. 6e). Additionally, we also noted that there was a considerable increase in the percentage of stage XIV tubules during which meiosis took place because meiosis failed to undergo completion with the needed support of actin-based cytoskeleton that conferred the meiotic spindles (Fig. 6f).
Knockdown of Spire 1 in the testis perturbs F-actin organization
In control testes, F-actin was prominently detected at the apical ES and basal ES (Fig. 7a). At the apical ES, F-actin appeared as bulb-like structure, intensely localized at the concave (ventral) side of spermatid heads in stage VII and VIII tubules, but considerably diminished in late stage VIII tubules and expressed prominently again in stage IX tubules in step 9 spermatids at the front end of spermatid head surrounding the developing acrosome (Fig. 7a). Also noted are the track-like structures conferred by F-actin in late stage VIII tubules (see white arrowheads, Fig. 7a). F-actin was notably expressed at the BTB, located just above the basement membrane (annotated by a dashed white line) (Fig. 7a). However, following Spire 1 knockdown, there were gross disruption of F-actin networks in all stages of the tubules, as illustrated in stages VII–IX tubules shown in Fig. 7a. For instance, F-actin no longer appeared as bulb-like structure intensively localized at the concave side of spermatid heads but randomly expressed around the spermatid heads (Fig. 7a). Furthermore, step 19 spermatids remained trapped deep inside the epithelium in stage VII and VIII tubules, and had mis-organized F-actin wrapped around these spermatids, unlike the control testis wherein F-actin appeared as bulb-like structures localized at the concave side of spermatid heads (Fig. 7a). In late stage VIII and IX tubules, following Spire 1 knockdown, F-actin also grossly disorganized, dissimilar from control testes, (Fig. 7). For instance, no track-like structures conferred by F-actin were detected after Spire 1 knockdown. When changes in F-actin organization was used to assess the defective tubules, a considerable increase in defective tubules was noted following Spire 1 knockdown vs. the control group (Fig. 7b).
Knockdown of Spire 1 in the testis perturbs protein distribution at the basal ES/BTB
Following Spire 1 knockdown in the testis in vivoin vivo, considerable changes in the distribution of basal ES proteins N-cadherin and ß-catenin, and also TJ proteins occludin and ZO-1, were noted at the BTB, located above the basement membrane (annotated by the white dashed line) (Fig. 8). These basal ES and TJ proteins no longer tightly localized at the BTB as noted in control testes, instead, they were diffusely localized at the BTB (Fig. 8, see also histograms on the right panel), possibly due to disruptive changes in the organization of F-actin.
Spire 1 knockdown perturbs F-actin organization in the testis through changes in the spatial expression of actin regulatory proteins Arp3 and Eps8
The efficacy of Spire 1 knockdown in the testis in vivo was confirmed by IF as noted in the top panel in Fig. 9a (see also histogram on the upper right panel). Changes in the organization of F-actin across the epithelium as noted in Fig. 7 were likely mediated through changes in the spatial expression of Arp3 and Eps8, which no longer robustly expressed at the concave side of the spermatid heads and restrictively expressed at the basal ES/BTB (Fig. 9). Instead, the expression of these two proteins were considerably diminished and also mis-localized compared to control testes (Fig. 9), thereby failing to support the preparation of elongated spermatids for their release at spermiation in late stage VIII tubules as well as their proper transport across the epithelium. This notion was supported by considerable reduction in the spatial expression of an apical ES protein laminin-γ3 chain at the spermatid heads (Fig. 9). The defects in F-actin organization thus induced premature release of elongated spermatids residing near the tubule lumen, but for those elongated spermatids that were near the base of the epithelium, they failed to be transported across the epithelium but remain trapped at the site (Fig. 9, red arrowheads). These changes that failed to support spermiation, altering the relative percentage of stage VII vs. VIII tubules, making it difficult to distinguish stage VII from putative stage VIII tubules due to premature spermiation, but also entrapment of step 19 spermatids in stage VIII tubules. For instance, in normal control testes (i.e., transfected with non-targeting negative control siRNA duplexes), VII and VIII tubules were found to be ~24% and ~7%, respectively, consistent findings of earlier reports51,52; but in Spire 1 knockdown testis, stage VII and VIII tubule were 15% and ~13%, respectively (Fig. 9, right panel).
Spire 1 knockdown perturbs MT organization in the testis
Spire 1 knockdown in adult rat testes in vivo also perturbed the organization of MTs based on studies using IF (Fig. 10a) and IHC (Fig. 10b). MTs appeared as track-like structures that laid across the seminiferous epithelium and aligned perpendicular to the basement membrane (annotated by dashed white line) in all staged tubules as shown in control testes in representative stage VII, VIII, IX, and XI tubules (Fig. 10a, b). Following Spire 1 knockdown, MT organization was grossly disrupted. For instance, the MT-conferred tracks where grossly truncated, and for those MTs that remained appeared as considerably shortened tracks and were also misaligned since they no longer laid perpendicular to the basement membrane as in control testes (Fig. 10). Disrupted MT organization likely caused by the disrupted spatial expression of EB1, a known MT stabilizing +TIP protein53. For instance, EB1 was grossly truncated and misaligned in the epithelium after Spire 1 knockdown when visualized by IF (Fig. 10a) and IHC (Fig. 10b).
Spire 1 knockdown perturbs the Sertoli cell BTB integrity in vivo
Findings shown in Figs. 7–10 support the notion that a knockdown of Spire 1 would perturb the BTB in vivo, we thus performed the BTB integrity assay to examine this possibility. As shown in Fig. 11, a knockdown of Spire 1 indeed perturbed the Sertoli cell BTB function in vivo (Fig. 11a), and this disruptive effect is statistically significant as shown in Fig. 11b.
Spire is as an actin nucleator known to generating long stretches of linear actin microfilaments efficiently to support epithelial and endothelial cell function4,22,54,55,56. Spire either works alone by interacting with the barbed end of the actin filament57 or it works in concert with formin to create the Spir/formin actin nucleator complex2,58,59 to induce actin polymerization. The actin polymerization activity in Spire is conferred by the central repeat of four WH2 (WASP-homology 2) domains containing the consensus sequence of LKKV commonly found in most actin filament nucleators, such as Spire 14,58,60,61. Thus, it is not surprising to see that Spire 1 is robustly expressed in the apical ES and basal ES ultrastructures since these are the unique actin-rich adherens junctions in the testis. Furthermore, the ES is supported by an extensive network of actin microfilament bundles that lay adjacent to the MT network, both of which undergo extensive remodeling, namely disassembly (depolymerization or cleavage) and reassembly (polymerization/nucleation) to confer plasticity to the ES to support germ cell transport and endocytic vesicle-mediated intracellular protein trafficking12,14,62,63. In short, apical and basal ES are dynamic AJ structures that require continuous remodeling to support the transport of spermatids and preleptotene spermatocytes across the adluminal compartment and the BTB, respectively, illustrating actin microfilaments that are assembled in bundles to support ES function undergo rapid re-organization. On the other hand, Spire 1 is co-localized, virtually superimposable, with apical ES constituent proteins such as β1-integrin45, nectin 246 and nectin 346 at the convex side of spermatid heads; and also basal ES proteins N-cadherin64 and γ-catenin at the BTB. These ES proteins are known to utilize actin microfilaments for their attachments65. In short, the precise co-localization of Spire 1 with these apical and basal ES cell adhesion protein complexes as noted herein is necessary to confer actin and possibly MT dynamics to support spermatogenesis. Thus, it is not unexpected that a knockdown of Spire 1 in Sertoli cells in vitro or in the testis in vivo was found to perturb the organization of F-actin network, impeding spermatid adhesion function, leading to their premature exfoliation from the seminiferous epithelium, but also perturbing spermatid transport across the epithelium. Also, some step 19 spermatids remained trapped deep inside the epithelium in stages IX, X, XI, and even XII tubules when they should have been released into the tubule lumen at stage VIII of the epithelial cycle. This is likely due to the loss of track-like structure conferred by actin filaments, and also the loss of tracks conferred by MTs, following Spire 1 knockdown, which are known to support cellular transport in other epithelia11,12,66,67. At present, it is not known if Spire 1 is working in concert with formin 1 as a Spire 1/formin 1 nucleation complex in the testis to support spermatogenesis. On the other hand, studies have also shown that Spire 1 is working with small GTPase Rab11 and actin-specific motor protein myosin Vb  as well as Rab 3A to modulate actin dynamics68. The possibility that Spire 1 is interacting with these other possible partners to mediate actin nucleation in the seminiferous epithelium during the epithelial cycle will require further investigations. Also, it is not known if the phenotypes noted herein after knockdown of Spire 1 were due to a disorganization of either actin, MT or both since the organization of both cytoskeletons was perturbed. However, it is likely that the actin-based cytoskeleton is the primary target of Spire 1 knockdown because Spire 1 is an actin nucleator. The primary disruption of actin organization thus impedes intracellular protein trafficking that causes a secondary disruption of MT organization. This possibility, however, must be carefully evaluated in future studies.
In this context, it is of interest to note that our findings that the mRNA of Spire 1 and Spire 2 genes are highly expressed in the testis are consistent with earlier reports69,70. Spire 1 and Spire 2 proteins were also shown to interact with formin 2 to facilitate proper alignment of metaphase spindle to support oocyte maturation during meiosis I in oocytes69. Interestingly, formin 2 deficient female mice (Fmn2−/− female mice) were sub-fertile, having recurrent pregnancy loss due to polyploid embryo formation71. However, both Spire 1 and Spire 2 genes had to be knocked down to generate the Fmn2−/− phenotype in oocytes69, suggesting Spire 1 and Spire 2 may have some redundant function. As such, it is not surprising to note that Spire 1 KO mice using a gene trap approach to obtain the mutant mice, both male and female mice were fertile72. One would argue if Spire 2 could supersede the lost function of Spire 1, why would a transient knockdown of Spire 1 lead to the phenotypes detected in the testis as reported herein? This is likely that while both Spire 1 and Spire 2 are present in the testis to support spermatogenesis, a sudden and unexpected loss of Spire 1 that led to the noted phenotypes—defects in spermatid and phagosome transport and adhesion, as well as loss of spermatid polarity due to a disorganization of actin- and MT-based cytoskeletons—would take time for the Spire 2 to kick-in the lost function of Spire 1, thereby revealing the significance of Spire 1 to support spermatogenesis. This conclusion is supported by the finding that the steady-state mRNA level of Spire 2 in the Spire 1 knockdown testis was unaffected. Furthermore, both in vitro and in vivo findings are consistent with each other that a knockdown of Spire 1 would impede both F-actin and MT organization in Sertoli cells in the testis. Furthermore, the phenotypes detected in the testis following Spire 1 knockdown also mimic those detected in an earlier report when formin 1 was silenced by ~70% in the testis20,21, supporting the notion that Spire1/formin 1 may be a crucial actin nucleator complex to support spermatogenesis.
It was unexpected that a knockdown of Spire 1 in Sertoli cells cultured in vitro or in the testis in vivo perturbed MT organization. It is possible that the disruptive effects of Spire 1 on MT organization was the result of a transient loss of Spire 1 actin nucleation activity following its knockdown which impeded F-actin-dependent intracellular protein trafficking. This thus caused a disruptive spatial expression of MT regulatory proteins, such as EB1 and/or MARKs, which in turn could perturb MT organization, leading to disruptive changes in MT organization. These possibilities must be carefully evaluated in future studies.
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This work was supported by grants from the National Institutes of Health (NICHD R01 HD056034 to C.Y.C.; U54 HD029990 Project 5 to C.Y.C.); Hong Kong Research Grants Council General Research Fund (GRF) (GRF171000816 to W.Y.L.; GRF771513 to W.M.L.); University of Hong Kong CRCG Seed Fund for Basic Research to W.M.L.; China Qianjiang Talents Program (QJD1502029 to X.X.). W.Q. was supported by fellowships from The F. Lau Memorial Fellowship, The Economic Development Council, and the Noopolis Foundation.
Conflicts of interest
The authors declare that they have no conflict of interest.
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Wen, Q., Li, N., Xiao, X. et al. Actin nucleator Spire 1 is a regulator of ectoplasmic specialization in the testis. Cell Death Dis 9, 208 (2018). https://doi.org/10.1038/s41419-017-0201-6
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