Fig. 6: PAI-1 derived from activated stromal fibroblasts promotes tumor cell proliferation and cell polarity change of MCF-7 | Cell Death & Disease

Fig. 6: PAI-1 derived from activated stromal fibroblasts promotes tumor cell proliferation and cell polarity change of MCF-7

From: Primed atypical ductal hyperplasia-associated fibroblasts promote cell growth and polarity changes of transformed epithelium-like breast cancer MCF-7 cells via miR-200b/c-IKKβ signaling

Fig. 6

a Venn diagram to show the identified target genes and the cytokines regulated by NF-κB in our published microarray data. b, c ELISA analysis to determine the secreting protein levels of MMP9 and PAI-1 in NFs, AHFs, and CAFs (b), or PAI-1 in the indicated fibroblasts (c). d MCF-7 cells were cultured alone or co-cultured with supernatant derived from NFs or CAFs under treatment as shown. The MCF-7 cell amounts are shown by histogram. The used concentration of PAI-1:50 µM; tiplaxtinin (Tipla): 30 µM. e Histogram to show the percentages of S-phase cells in cell cycle for MCF-7 co-cultured with supernatant derived from NFs or CAFs under the treatment as shown. The used concentration of PAI-1 and tiplaxtinin are as in d. fh Cell culture and treatments of MCF-7 cells are as same as described in e. Western blotting analysis (f) and Immunofluorescent staining (g) to detect E-Cadherin and Vimentin expressions in MCF-7. Cell invasion of MCF-7 (h) was analyzed by Transwell chamber analysis (magnification ×200). The data were shown as mean ± SD for N ≥ 3 separate experiments, *p < 0.05. MMP9 matrix metalloproteinase 9, PAI-1 plasminogen activator inhibitor-1, Tiplaxtinin (Tipla) PAI-1 inhibitor

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