Fig. 5: NF-κB enhances stromal fibroblasts activation | Cell Death & Disease

Fig. 5: NF-κB enhances stromal fibroblasts activation

From: Primed atypical ductal hyperplasia-associated fibroblasts promote cell growth and polarity changes of transformed epithelium-like breast cancer MCF-7 cells via miR-200b/c-IKKβ signaling

Fig. 5

a, b IHC staining (a) and quantitation (b) of IKKβ, IκBα, p-IκBα, and nuclear P65 protein levels in human normal mammary tissues (normal), breast atypical ductal hyperplasia (ADH), and breast ductal carcinoma in situ (DCIS). The black arrows indicate distinctly stained fibroblasts in the representative tumor tissues. Scale bars, 100 μm. c Western blotting analysis of α-SMA and FSP1 expressions in NFs treated with or without TNF-α and CAPE. GAPDH was used as a loading control. d and e The cell count (d) and the percentages of S-phase cells in cell cycle (e) were shown by histogram in NFs treated with or without TNF-α and CAPE. f Transwell chamber analysis for detection of the cell invasion of NFs treated with or without TNF-α and CAPE. The data were shown as mean ± SD for N ≥ 3 separate experiments, *p < 0.05. TNF-α tumor necrosis factor-α, CAPE caffeic acid phenethyl ester, NF-κB inhibitor

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