Fig. 3: Decreased miR-200b/c induce activation of stromal fibroblasts | Cell Death & Disease

Fig. 3: Decreased miR-200b/c induce activation of stromal fibroblasts

From: Primed atypical ductal hyperplasia-associated fibroblasts promote cell growth and polarity changes of transformed epithelium-like breast cancer MCF-7 cells via miR-200b/c-IKKβ signaling

Fig. 3

a The different miRNAs among NFs, AHFs, and CAFs were identified by miRNA array analysis. b The expressions of miR-200b/c identified by miRNA array were confirmed using qRT-PCR in NFs, AHFs, and CAFs. U6 was used as an internal control. c Western blots of α-SMA and FSP1 expressions are shown in the indicated engineered CAFs or NFs; GAPDH was used as a loading control. d, e The cell count (d) and the percentages of S-phase cells in cell cycle (e) are shown by histogram in the indicated engineered CAFs or NFs. f, g Transwell chamber analysis to test the cell invasion potential of the indicated engineered CAFs or NFs (magnification ×200). The invaded cells are shown by histogram (g). The data were shown as mean ± SD for N ≥ 3 separate experiments, *p < 0.05

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