Fig. 2: AHFs stimulate cell growth and polarity change of MCF-7 under co-culture conditions | Cell Death & Disease

Fig. 2: AHFs stimulate cell growth and polarity change of MCF-7 under co-culture conditions

From: Primed atypical ductal hyperplasia-associated fibroblasts promote cell growth and polarity changes of transformed epithelium-like breast cancer MCF-7 cells via miR-200b/c-IKKβ signaling

Fig. 2

a A sketch to depict the 3D co-cultured system of fibroblasts and MCF-7. b Cell proliferation of MCF-7 was determined by cells count under co-culture system with stromal fibroblasts (NFs, AHFs, or CAFs). c The percentage of S-phase cells in cell cycle was shown for MCF-7 alone or MCF-7 co-cultured with NFs, AHFs, or CAFs. d Western blotting analysis to determine the E-Cadherin and Vimentin expression in MCF-7 or MCF-7 co-cultured with NFs, AHFs, or CAFs; GAPDH was used as a loading control. e, f Immunofluorescence staining (e) and quantitation (f) of E-cadherin and Vimentin expression in MCF-7 or MCF-7 co-cultured with NFs, AHFs, or CAFs. g Transwell chamber analysis to detect the invaded cells for MCF-7 or MCF-7 co-cultured with NFs, AHFs, or CAFs (magnification ×200). h Indicating tumor size in mice injected with MCF-7 cells alone or MCF-7 cells mixed with the indicated stromal fibroblasts. i Ki-67 staining in the xenograft samples. The white arrows indicate distinctly stained cells in the representative xenograft tissues. Scale bars, 100 μm. The data were shown as mean ± SD for N ≥ 3 separate experiments, *p < 0.05