Fig. 1: AHFs are one kind of activated myofibroblasts in mammary atypical hyperplasia tissues and possess essential biological characteristics to CAFs and NFs | Cell Death & Disease

Fig. 1: AHFs are one kind of activated myofibroblasts in mammary atypical hyperplasia tissues and possess essential biological characteristics to CAFs and NFs

From: Primed atypical ductal hyperplasia-associated fibroblasts promote cell growth and polarity changes of transformed epithelium-like breast cancer MCF-7 cells via miR-200b/c-IKKβ signaling

Fig. 1

a, b IHC staining (a) and quantitation (b) of α-SMA and FSP1 expressions in human normal mammary tissues (normal), breast atypical ductal hyperplasia (ADH), and breast ductal carcinoma in situ (DCIS). The black arrows indicate distinctly stained fibroblast cells in the representative tissues. Scale bars, 100 μm. c, d Immunofluorescence staining (c) and quantitation (d) of α-SMA and FSP1 expressions in primary fibroblasts derived from normal mammary tissues (NFs), mammary atypical hyperplasia (AHFs), and breast ductal carcinoma in situ (CAFs). e Cell proliferation determined by MTT assay for NFs, AHFs, and CAFs. f The percentages of S-phase cells in cell cycle are shown by histogram for NFs, AHFs, and CAFs. g Transwell chamber analysis to determine cell invasion abilities of NFs, AHFs, and CAFs. The data were shown as mean ± SD for N ≥ 3 separate experiments, *p < 0.05