While inner ear disorders are common, our ability to intervene and recover their sensory function is limited. In vitro models of the inner ear, like the organoid system, could aid in identifying new regenerative drugs and gene therapies. Here, we provide a perspective on the status of in vitro inner ear models and guidance on how to improve their applicability in translational research. We highlight the generation of inner ear cell types from pluripotent stem cells as a particularly promising focus of research. Several exciting recent studies have shown how the developmental signaling cues of embryonic and fetal development can be mimicked to differentiate stem cells into “inner ear organoids” containing otic progenitor cells, hair cells, and neurons. However, current differentiation protocols and our knowledge of embryonic and fetal inner ear development in general, have a bias toward the sensory epithelia of the inner ear. We propose that a more holistic view is needed to better model the inner ear in vitro. Moving forward, attention should be made to the broader diversity of neuroglial and mesenchymal cell types of the inner ear, and how they interact in space or time during development. With improved control of epithelial, neuroglial, and mesenchymal cell fate specification, inner ear organoids would have the ability to truly recapitulate neurosensory function and dysfunction. We conclude by discussing how single-cell atlases of the developing inner ear and technical innovations will be critical tools to advance inner ear organoid platforms for future pre-clinical applications.
Over 6% of people worldwide suffer from hearing loss  and likewise 6% suffer from balance disorders . Both these sensory systems are located in the inner ear, which can be affected by the aging process, genetic mutations, infectious diseases, chronic infections, noise exposure, and ototoxic drugs [1, 2]. Despite the prevalence of inner ear sensory dysfunction, which in the case of hearing loss is irreversible, there are currently no approved medications specifically targeting sensory recovery. Devices, such as hearing aids or cochlear implants, are commonly used to manage—not cure—moderate to severe hearing loss cases. In the hunt for new therapies, in vitro human, three-dimensional and multicellular systems mimicking the inner ear, that is inner ear organoids, could be a useful tool to accelerate therapeutic discovery. Such models could be used to test virus-mediated gene therapies for congenital hearing loss disorders [3, 4], and screen for compounds to regenerate sensory cells following ototoxic degeneration . Additionally, human organoid models will provide insight into unique human aspects of inner ear development and pathologies, which are impossible to gain from animal studies. Despite several recent breakthroughs, application of cellular models of the inner ear have been slow to gain momentum for disease models and as drug discovery platforms. In this perspective article we will briefly discuss progress toward in vitro inner ear modeling approaches, which has been thoroughly covered in recent reviews [3, 6,7,8,9,10,11]. Then, we will focus on diagnosing key barriers to progress and discuss advances from other fields that shed light on how more complete, scalable, and reproducible inner ear organoid models can be built.
The challenge of modeling inner ear development in vitro
Different approaches of modeling the human inner ear in vitro have been demonstrated using human pluripotent stem cells (hPSCs) [12,13,14,15,16,17,18,19,20,21,22,23,24], adult tissue resident stem cells , or fetal progenitor stem cells [26, 27]. Unlike some other organ systems (e.g., the intestines), the routine use of patient-derived tissue for research is not scalable, because the inner ear is difficult to biopsy and refractory to long-term culture [28, 29]. Furthermore, the use of fetal tissue is tightly regulated in some countries and the unpredictability of specimen collection complicates downstream analysis . In contrast, hPSCs are potentially an endless source of cellular tissue for experimentation and can be genetically manipulated (e.g., introduction of pathologic genetic mutations, or the generation of reporter cell lines). There are two types of hPSCs: embryonic stem cells (ESCs) derived from pre-implantation embryos and induced pluripotent stem cells (iPSCs) derived from reprogrammed adult cells. hPSCs can be differentiated to otic progenitor cells and more mature inner ear cell types by mimicking embryonic and fetal development. These differentiation approaches have been recently reviewed [7, 8, 10]. In the embryo, inner ear development requires the assembly of diverse cell types from multiple cell lineages: the epithelial, neuronal, and glial cells of the inner ear are derived from the ectoderm germ layer, whereas the specialized periotic mesenchyme (POM) that surrounds the inner ear arises from the mesoderm germ layer and cranial neural crest—a population of cells that generate neurons, glia, melanocytes, and mesenchyme in the head and neck . A major long-term bioengineering challenge is to incorporate all of these multiple cell lineages into an inner ear organoid in vitro.
During early embryogenesis, pluripotent cells in the epiblast generate the ectoderm germ layer, which splits into the non-neural (also called surface) ectoderm and neuroectoderm. The otic placode arises at the border region between the cranial non-neural ectoderm and neuroectoderm, known as the otic-epibranchial placode domain (OEPD) [31, 32]. Several signaling pathways, including fibroblast growth factors (FGFs), WNT, transforming growth factor-beta (TGF), bone morphogenetic proteins (BMPs), sonic hedgehog (SHH), and retinoic acid (RA), are involved in this early otic cell fate specification. TGF, FGF, and WNT signaling appear to be the most essential morphogenic cues to form the OEPD in the embryo [33,34,35]. During otic placode formation, WNTs secreted from cranial mesenchyme and neural tube activate NOTCH pathway signaling in the surface ectoderm . A negative feedback loop in turn, downregulates FGF signaling to further specify otic progenitors within the placode . The otic placode subsequently invaginates to form the otocyst (also known as the otic vesicle), which gives rise to the majority of epithelial cell types in the vestibular and cochlear compartments of the inner ear. In addition to otic placode derived-cells, lineage tracing experiments in chickens and mice have shown that a limited number of neural crest cells contribute to the otocyst epithelium; however, it remains unclear what contribution these cells have to non-sensory and sensory epithelia in the inner ear later in development [38, 39]. Based on these developmental biology studies, a consensus model of otic induction from PSCs has emerged in recent years (Fig. 1a). Induction of otic and other cranial placodes, however, show limited efficiency in comparison to other ectodermal lineages in two-dimensional monolayer cultures [40,41,42]. In our previous study, we have shown that multiple otic placodes and otocyst-like structures can be generated in vitro from a three-dimensional (3D) hPSC aggregate by modulating TGF, BMP, FGF, and WNT signaling and extracellular matrix-related mechanical interactions (Fig. 1a) . The floating 3D culture appears to be ideal for allowing differentiating ectoderm and otic lineage cells to undergo the complex morphogenetic changes required for placode generation. Interestingly, using a modified version of our otocyst culture system, we recently demonstrated the generation of hair-bearing skin organoids—like the inner ear, hair follicle induction involves formation of epithelial placodes . Our understanding of the biomechanics of epithelial placode induction is limited and worthy of future exploration to gain insight into how to improve in vitro placode induction approaches [44,45,46].
Upon embryonic otocyst formation, asymmetric gene expression leads to dorsoventral and anteroposterior patterning that sets up the coordinates for subsequent development of the semicircular canals, endolymphatic sac, vestibular organs, and cochlea . Signals from surrounding tissues—the notochord, neural tube, and cranial mesenchyme—are crucial for setting up the axes of the otocyst . SHH secreted from the notochord and neural tube floor plate has been shown to establish the ventral part of the otocyst, giving rise to the cochlea, saccule and vestibulocochlear ganglion [49,50,51]. The graded dorsoventral SHH signaling is accomplished by a competing mechanism between GLI activators and GLI repressors. SHH knockout experiments have shown that attenuation of SHH within the mouse otocyst has little effect on the development of dorsal tissues, such as the semicircular canals, ampullae and utricle, but in contrast has a detrimental effect on ventral induction, highlighting the importance of SHH signaling for cochlea formation . Meanwhile, the WNT and BMP signaling molecules have been shown to act as dorsalizing cues in the mouse otocyst . The neurosensory region of the otocyst gives rise to delaminating neuroblasts and the sensory epithelia. Formation of this region is mediated by NOTCH and SOX2 activity downstream of WNT signaling . Division of the neurogenic and prosensory zones of the otocyst is mediated by transcription regulators, such as TBX1––a negative regulator of neurogenesis that acts downstream of SHH, WNT, and RA signals . Following otocyst patterning, the anteroventral prosensory domain begins to elongate to form the cochlear duct, and eventually forms the basal portion of the organ of Corti in the cochlea. During elongation and coiling of the cochlear duct, elaborate signaling dynamics fine tune the cellular diversity of the organ of Corti. The boundary between organ of Corti’s Kölliker organ (neural) and outer sulcus is regulated by levels of BMP4 expression; cells exposed to moderate levels of BMP4 signaling will adopt a prosensory cell fate, whereas under higher levels, cells will adopt a non-sensory cell fate . Extrinsic SHH signaling arises from the cochleovestibular ganglion, and the inhibition of this signal stimulates cochlear prosensory expansion . In addition, FGF signaling is essential to regulate the width of the prosensory domain , and NOTCH plays a role in lateral induction of cochlear sensory cells [59, 60]. A recent study has also revealed a role for Hippo signaling in progenitor cell renewal, thus controlling the size of the organ of Corti . Similarly, it has been shown that patterning of the vestibular system occurs by coordinated interactions of the NOTCH, WNT, BMP, FGF, and RA signaling pathways [62,63,64,65,66]. Strikingly little is known about the signaling mechanisms that underlie non-sensory epithelial specification in the ear; however, retinoic acid seems to be involved in the formation of non-sensory ionic regulatory epithelia in both the cochlea and vestibular end organs [67, 68].
Thus, the inner ear is patterned through the integration of a multitude of signaling pathways across space and time. These signals arise from within epithelia and from surrounding tissues, which enables the differentiation of otic progenitor cells to subsequent cochlear and vestibular fates. Our collective knowledge of these mechanisms has come from animal models and few, if any, mechanistic studies have been performed on human fetal inner ear tissues (see Roccio et al.  for an exception). To some degree, spatiotemporal signaling cues can be mimicked in hPSC three-dimensional cultures using bath application of recombinant proteins and small molecules, which can stimulate self-assembly of inner ear epithelia and neuron complexes (as demonstrated in Koehler et al. ); however, this approach is difficult to control and the generated organoids take on irregular shapes/sizes and contain an unpredictable mix of sensory and non-sensory cells. In future studies, more sophisticated 3D bioprinting and/or microfluidic-based approaches may be necessary to establish spatially controlled cellular structures that can be acted on by signaling gradients—resulting in an inner ear organoid-on-chip. Recent studies using microfluidic or microwell systems to pattern hPSCs into multilineage embryo-like, renal, or intestinal structures could be a guide toward improving control and reproducibility of otic induction [69,70,71,72].
Constructing inner ear organoids: what cells are missing?
The cellular composition of the inner ear is remarkably diverse, containing over fifty distinct cellular subtypes, including hair cells, supporting cells, non-sensory epithelial cells, as well as unique neurons and mesenchymal populations [73, 74]. Current hPSC-derived models only appear to contain a small subset of these cell types or, perhaps, enclose greater cellular diversity that has not been sufficiently characterized [12,13,14,15,16,17,18,19,20,21,22,23,24] (Fig. 1b). Due to their prevalence in human inner ear disorders, the main focus of characterization has been on neurosensory cell types. For instance, the hair cells generated from hPSCs to date are predominantly vestibular in character [13, 15,16,17, 21,22,23,24]. In correspondence with the neurosensory bias in defining these models, neurons [12,13,14, 16, 19, 21,22,23,24] and glial cells are described [12, 19, 21], with some studies describing synapse formation between the generated hair cells and neurons [12, 13, 19, 21, 22]. However, a clear distinction between vestibular and cochlear neurons has not been made; thus, we do not yet have a complete picture of native inner ear cell types that are recapitulated in inner ear models.
We contend that fully defining the cell populations of inner ear organoid models will be important for properly mimicking human inner ear disorders in vitro. Although a large subset of the >150 genes associated with hereditary deafness impact the function of hair cells, many of these genes are expressed in non-sensory or mesenchymal cell populations . Recent studies using mPSCs prove the capability of studying hearing loss caused by dysfunction of Gap Junction Protein Beta 2 (GJB2) , BarH Like Homeobox 1 , and Transmembrane Protease Serine 3 (TMPRSS3) . Moreover, hiPSCs have been used to study MYO15A  and MYO7A , as well as SLC26A4 [20, 81]. These studies, however, focus on specific neurosensory-related cell types, including hair cells [77, 79, 80], cochlear supporting cells , and cochlear outer sulcus cells , rather than describing a functional unit containing multiple inner ear cell types. Overall, the neurosensory bias of the PSC-related literature makes it unclear to what extent PSC-derived inner ear cells or organoids reflect the true cellular diversity of the inner ear. Thus, the potential disease modeling applications are limited. For instance, there are cochlear and vestibular diseases, as well as certain ototoxic agents, that have their pathological impact—either directly or indirectly—on non-sensory cells of the inner ear [4, 75, 82,83,84,85]. Therefore, it will be essential to generate inner ear models that contain a diverse functional unit of inner ear cell types to capture complex genetic or drug-related mechanisms. In the following sections, we will provide more insight into the different cell types forming the functional unit of the inner ear and give directions for future organoid research.
A primary goal of inner ear organoid generation is the production of hair cells. Although the majority of hair cells are reported to be vestibular-like [21, 22, 24], one report suggests the presence of cochlear-like outer hair cells based on the expression of SLC26A5 . Further investigation into the specific subtype of these presumptive cochlear outer hair cells must be established by functional analysis or reviewing additional protein expression, bearing in mind the temporal and spatial expression of certain hair cell markers . Additionally, hair cells do not function on their own; normal hair cell function is dependent on the endolymphatic electrical potential, which is maintained by the surrounding ionic regulatory epithelia , a subset of non-sensory epithelial cells. These epithelia include the stria vascularis in the cochlea and the dark cell area in the vestibular organs, which function together with surrounding epithelia and POM in ion recycling and endolymph homeostasis . Cell types within these ionic regulatory epithelia have distinct embryonic origins, which will complicate our ability to model them in vitro. In the stria vascularis, marginal cells arise from the otocyst, intermediate cells from the neural crest, and basal cells from the POM [88,89,90]. Similarly, the vestibular dark cells arise from the otocyst and associated melanocytes from the neural crest . Dysfunction of these epithelia can lead to metabolic hearing loss and vestibular dysfunction [92, 93]. The ionic regulatory cell types have not been described yet in hPSC-derived inner ear models. Our group recently showed that melanocytes arise in a skin organoid model that shares many induction steps and cellular components with the inner ear organoid model ; thus, it may be possible to co-induce the otocyst-derived and neural crest-derived components of ionic regulatory epithelia.
Cochlear outer sulcus-like cells, as part of the non-sensory epithelia, could be derived from human iPSCs . The sulcus-like cells were identified by their expression of various markers, including the anion exchanger SLC26A4. However, these markers are expressed in a variety of epithelial cells throughout the inner ear, such as the endolymphatic sac, the vestibular transitional cells, and outer-sulcus cells . Moreover, cell type-specific proteins might be expressed by different cell types during fetal development before becoming restricted to a specific cell type at a later developmental stage [89, 91, 95]. In future work, it will be beneficial to find markers that differentiate between these cell populations before assigning a definitive cochlear, vestibular, or endolymphatic identity. In general, the field should make use of techniques, such as single-cell RNA sequencing or mass cytometry, instead of relying on single marker genes or proteins to define inner ear cell fates that undergo dynamic spatiotemporal changes during development.
Neurons and glia
The formation and maintenance of synapses between hair cells and spiral ganglion neurons (the cochlear neurons) plays an important role in functional development and is linked with noise-induced hearing loss . Recent studies have demonstrated that inner ear spiral ganglion-like neurons can be generated from hPSCs in 2D culture  as well as the organoid model . However, it remains unclear how closely neurons in these systems mimic the gene expression and functional signatures of bone fide spiral ganglion neurons. The inner ear arises in a milieu rich with sensory neurons (cranial nerves V, VII, VIII, IX, and X) derived from placodes (the otic, epibranchial, and trigeminal placodes) and cranial neural crest cells; thus, it is critical to distinguish between these other possible neuron subtypes during the PSC derivation process. To date, a lack of reference data and limited markers has made it difficult to discern neurons of otic, epibranchial, and neural crest origin. Recent single-cell RNA-sequencing studies on various head and neck peripheral neurons should be a valuable resource to better define PSC-derived otic neurons relative to other cranial neurons [97,98,99]. Beyond characterizing molecular signatures, it will be important to elucidate the function properties of organoid neurons. It is well known that spontaneous activity occurs in the auditory and vestibular organs during development . Recently, independent research groups linked spontaneous activity in hair cells to cell fate specification in nascent spiral ganglion neurons [101, 102]. It will be insightful to confirm that spontaneous activity is present during organoid maturation and also to validate that organoid-derived neurons undergo subtype specification in a similar manner to the native inner ear.
Schwann cells are the myelinating cells of peripheral nerves, including the VIII cranial nerve in the inner ear. Unlike inner ear neurons, which arise from the otocyst, Schwann cells arise from the cranial neural crest. Remarkably, the inner ear organoid model appears to co-produce S100B + Schwann cells that closely associate with sensory neurons in the culture (Fig. 1a) . The characterization of Schwann cells in this model remains limited. For instance, it is not known whether organoid Schwann cells actually begin to express myelin basic protein (MBP) and myelinate organoid neurons. It will be important to further elucidate the identity and function of Schwann cells in the inner ear organoid system.
Organoids could also be used to investigate neural pathfinding and synapse formation of otic afferent neurons with central nervous system targets. A co-culture system of a developing inner ear organoid paired with developing hindbrain tissue, would potentially mimic elements of the otic ganglion-to-cochlear nucleus circuit . The type of complex interactions between the developing inner ear and hindbrain could be captured in this system in a similar manner to that performed with dorsal and ventral cerebral organoids in recent studies . A key barrier to building an inner ear-hindbrain organoid system is our lack of knowledge about the mechanisms needed for brainstem and, specifically, cochlear nucleus induction from pluripotent stem cells. Efforts toward single-cell mapping of neurons in the brainstem nuclei over developmental time, like a recent study on dorsal raphe neurons, will be critical for future progress .
Periotic mesenchyme (POM)
In addition to the role of POM in endolymph ionic homeostasis, it plays an important role in otocyst patterning . Shortly after invagination of the otic placode, at around E9.5 in mice or fetal week 4–5 in humans, the POM begins to form at the anteroventral pole of the otocyst . The POM is a specialized type of cranial paraxial mesoderm and neural crest , which expresses specific transcription factors, TBX1, TBX18, and POU3F4 [106,107,108,109]. Many of these genes are associated with deafness and/or vestibular dysfunction [67, 108, 110]. Over five different types of fibrocytes with unique protein expression and spatial localization arise from the POM [92, 93]. The POM gives rise to the cartilaginous and, later, bony otic capsule, as well as the temporal bone. Clearly, the POM is a critical component of normal inner ear morphogenesis. A detailed understanding of what types of mesenchymal cells arise in inner ear organoid cultures is lacking. Our published data suggest that inner ear organoid epithelia co-develop with a neural crest-derived mesenchyme that produces fibrocyte-like cells and cartilage, similar to the POM  (Fig. 1a). However, it is not known whether authentic POM—expressing the transcription factors mentioned above—arise in these cultures or whether the mesoderm-derived components of the POM are present. It will be important to define the organoid mesenchyme for future progress. Notably, the importance of organ-specific mesenchyme for higher-order organoid development and maturation has been highlighted in several recent publications on kidney, gut, and skin organoids [43, 111,112,113]. In particular, the organoid model could be used to study how reciprocal interactions between POM and epithelia impact specification and maturation of cochlear versus vestibular cell fates and cell type specification.
Vasculature and immune cells
The inner ear has a unique relationship to the rest of the body. In many ways it is isolated: largely immune privileged and with a vascular barrier system called the blood-labyrinth barrier (akin to the blood-brain barrier (BBB) in the central nervous system) . It is unclear what role the vasculature plays in inner ear organogenesis; however, blood flow through capillaries in the stria vascularis appears to be integral to maintenance of the endocochlear potential . An often-cited weakness of organoid models is the lack of vasculature; however, recent work has led to blood vessel organoids and incorporation of a BBB in cerebral organoids [116,117,118]. Likewise, these novel platforms could be leveraged to infuse inner ear organoids with endothelial cells and pericytes to investigate the role of vasculature in inner ear organ maturation. Incorporation of vasculature seems to enhance maturation of intestinal and kidney organoid systems [119,120,121]. Homan et al. shows that the introduction of endothelial cells and media flow actually enhances maturation in kidney organoids compared to static controls; however, the presence of vasculature alone was not enough to achieve maturation . Additionally, Palikuqi et al. recently described an organoid-compatible system with perfusable vessels . Such a system could be adapted to create, for instance, a stria vascularis-on-chip system. Introduction of endothelial cells and pericytes will alter cell–cell and cell–matrix interactions, and the additional supply of nutrients to areas deprived of physiological levels (due to the growth and large size of organoids), might beneficially affect maturation in an inner ear organoid system.
Most recently, it has been shown that forms of genetic hearing loss are linked to autoinflammation in the inner ear . Like many other organoid models, the utility of in vitro inner ear organoid systems may be expanded by incorporating key immune cell populations, perhaps generated from autologous iPSCs. A prime example of immune cell incorporation into organoid models has been the use of microglia in cerebral organoids [123, 124]. Microglia are the resident immune cells of the brain, and recently, researchers have successfully derived microglial cells by guiding hPSCs into the mesodermal lineage. In parallel, brain organoids were induced and seeded with microglia using a simple co-culture approach. The timing of seeding was chosen to reflect the timing of microglial migration to the brain during normal development. Similarly, a parallel-induction and seeding approach of immune cells could be employed to incorporate macrophages into inner ear organoids and set the stage for inflammation studies .
Conclusions and future directions
As we have discussed, major breakthroughs have been made in inner ear modeling using hPSCs, yet current models are limited in their applications for translational research. To induce greater cell diversity in inner ear organoid models, it is essential to identify key regulatory pathways that play a role in determination of vestibular versus cochlear, epithelial versus mesenchymal, and sensory versus non-sensory cell fate decisions. Manipulation of these pathways should be systematically incorporated into current differentiation protocols (Fig. 2). More fundamentally, cell type specific markers that can distinguish between vestibular versus cochlear cell populations at every stage of development are lacking. In this regard, single-cell resolution atlases of the developing inner ear will be an important tool to advance inner ear organoid platforms. Initial single-cell mapping efforts have focused on maturation of the cochlea, specifically, the organ of Corti epithelium [73, 126,127,128,129]. To improve PSC-based otic induction strategies, however, it will be beneficial to have broader single-cell reference atlases for the inner ear at various developmental timepoints. For instance, researchers should perform single-cell analysis on earlier stages of development (i.e., E8.5–E11.5) to encompass the critical period of otocyst axial and sensory versus non-sensory patterning. In addition, single-cell atlases including both cochlear and vestibular compartments of the inner ear would allow for comparative analysis between the two organ systems. Recent work on foregut differentiation has demonstrated how single-cell RNA-sequencing data from critical periods of development can be used to construct more refined cell lineages and infer epithelial–mesenchymal cell interactions . A similar approach could be applied to better define otic induction in the mouse embryo. Moreover, single-cell genomics data from human fetuses—as have been generated recently for the human fetal liver, skin, and inner ear [73, 130]––could be used to refine human otic induction methods. Together with the introduction of technical innovations, such as co-cultures and microfluidics, a more complete, scalable, and reproducible inner ear organoid model could be used to study inner ear dysfunction and sensory recovery for tomorrow’s medicine.
WHO. Fact sheet: deafness and hearing loss. https://www.who.int/news-room/fact-sheets/detail/deafness-and-hearing-loss. 2020; Accessed 1 Jul 2020.
Hülse R, Biesdorf A, Hörmann K, Stuck B, Erhart M, Hülse M, et al. Peripheral vestibular disorders: an epidemiologic survey in 70 million individuals. Otol Neurotol. 2019;40:88–95.
Géléoc GSG, Holt JR. Sound strategies for hearing restoration. Science. 2014;344:1241062.
Delmaghani S, El-Amraoui A. Inner ear gene therapies take off: current promises and future challenges. J Clin Med. 2020;9:2309.
Ganesan P, Schmiedge J, Manchaiah V, Swapna S, Dhandayutham S, Kothandaraman PP. Ototoxicity: a challenge in diagnosis and treatment. J Audiol Otol. 2018;22:59–68.
Perny M, Ting C-C, Kleinlogel S, Senn P, Roccio M. Generation of otic sensory neurons from mouse embryonic stem cells in 3D culture. Front Cell Neurosci. 2017;11:409.
Czajkowski A, Mounier A, Delacroix L, Malgrange B. Pluripotent stem cell-derived cochlear cells: a challenge in constant progress. Cell Mol Life Sci. 2019;76:627–35.
Roccio M, Edge ASB. Inner ear organoids: new tools to understand neurosensory cell development, degeneration and regeneration. Development. 2019;146:dev177188.
Roccio M, Senn P, Heller S. Novel insights into inner ear development and regeneration for refined hearing loss therapies. Hear. Res. 2019. https://doi.org/10.1016/j.heares.2019.107859.
Tang P-C, Hashino E, Nelson RF. Progress in modeling and targeting inner ear disorders with pluripotent stem cells. Stem Cell Rep. 2020;14:996–1008.
Durán-Alonso MB. Stem cell-based approaches: possible route to hearing restoration? World J Stem Cells. 2020;12:422–37.
Shi F, Corrales CE, Liberman MC, Edge ASB. BMP4 induction of sensory neurons from human embryonic stem cells and reinnervation of sensory epithelium. Eur J Neurosci. 2007;26:3016–23.
Chen W, Jongkamonwiwat N, Abbas L, Eshtan SJ, Johnson SL, Kuhn S, et al. Restoration of auditory evoked responses by human ES-cell-derived otic progenitors. Nature. 2012;490:278–82.
Gunewardene N, Bergen NV, Crombie D, Needham K, Dottori M, Nayagam BA. Directing human induced pluripotent stem cells into a neurosensory lineage for auditory neuron replacement. Biores Open Access. 2014;3:162–75.
Ronaghi M, Nasr M, Ealy M, Durruthy-Durruthy R, Waldhaus J, Diaz GH, et al. Inner ear hair cell-like cells from human embryonic stem cells. Stem Cells Dev. 2014;23:1275–84.
Ohnishi H, Skerleva D, Kitajiri S, Sakamoto T, Yamamoto N, Ito J, et al. Limited hair cell induction from human induced pluripotent stem cells using a simple stepwise method. Neurosci Lett. 2015;599:49–54.
Ding J, Tang Z, Chen J, Shi H, Chen J, Wang C, et al. Induction of differentiation of human embryonic stem cells into functional hair-cell-like cells in the absence of stromal cells. Int J Biochem Cell Biol. 2016;81:208–22.
Ealy M, Ellwanger DC, Kosaric N, Stapper AP, Heller S. Single-cell analysis delineates a trajectory toward the human early otic lineage. Proc Natl Acad Sci. 2016;113:8508–13.
Matsuoka AJ, Morrissey ZD, Zhang C, Homma K, Belmadani A, Miller CA, et al. Directed differentiation of human embryonic stem cells toward placode-derived spiral Ganglion-like sensory neurons. Stem Cell Transl Med. 2016;6:923–36.
Hosoya M, Fujioka M, Sone T, Okamoto S, Akamatsu W, Ukai H, et al. Cochlear cell modeling using disease-specific iPSCs unveils a degenerative phenotype and suggests treatments for congenital progressive hearing loss. Cell Rep. 2017;18:68–81.
Koehler KR, Nie J, Longworth-Mills E, Liu X-P, Lee J, Holt JR, et al. Generation of inner ear organoids containing functional hair cells from human pluripotent stem cells. Nat Biotechnol. 2017;35:583–9.
Jeong M, O’Reilly M, Kirkwood NK, Al-Aama J, Lako M, Kros CJ, et al. Generating inner ear organoids containing putative cochlear hair cells from human pluripotent stem cells. Cell Death Dis. 2018;9:922.
Lahlou H, Nivet E, Lopez-Juarez A, Fontbonne A, Assou S, Zine A. Enriched differentiation of human otic sensory progenitor cells derived from induced pluripotent stem cells. Front Mol Neurosci. 2018;11:452.
Mattei C, Lim R, Drury H, Nasr B, Li Z, Tadros MA, et al. Generation of vestibular tissue-like organoids from human pluripotent stem cells using the rotary cell culture System. Front Cell Dev Biol. 2019;7:25.
Senn P, Mina A, Volkenstein S, Kranebitter V, Oshima K, Heller S. Progenitor cells from the adult human inner ear. Anat Rec. 2020;303:461–70.
Chen W, Johnson SL, Marcotti W, Andrews PW, Moore HD, Rivolta MN. Human fetal auditory stem cells can be expanded in vitro and differentiate into functional auditory neurons and hair cell‐like cells. Stem Cells. 2009;27:1196–204.
Roccio M, Perny M, Ealy M, Widmer HR, Heller S, Senn P. Molecular characterization and prospective isolation of human fetal cochlear hair cell progenitors. Nat Commun. 2018;9:4027.
Taylor RR, Jagger DJ, Saeed SR, Axon P, Donnelly N, Tysome J, et al. Characterizing human vestibular sensory epithelia for experimental studies: new hair bundles on old tissue and implications for therapeutic interventions in ageing. Neurobiol Aging. 2015;36:2068–84.
Taylor RR, Filia A, Paredes U, Asai Y, Holt JR, Lovett M, et al. Regenerating hair cells in vestibular sensory epithelia from humans. Elife. 2018;7:e34817.
Trainor PA, Tam PP. Cranial paraxial mesoderm and neural crest cells of the mouse embryo: co-distribution in the craniofacial mesenchyme but distinct segregation in branchial arches. Development. 1995;121:2569.
Schlosser G, Patthey C, Shimeld SM. The evolutionary history of vertebrate cranial placodes II. Evolution of ectodermal patterning. Dev Biol. 2014;389:98–119.
Patthey C, Gunhaga L. Signaling pathways regulating ectodermal cell fate choices. Exp Cell Res. 2014;321:11–16.
Ladher RK, Anakwe KU, Gurney AL, Schoenwolf GC, Francis-West PH. Identification of synergistic signals initiating inner ear development. Science. 2000;290:1965–7.
Ohyama T, Groves AK. Generation of Pax2‐Cre mice by modification of a Pax2 bacterial artificial chromosome. Genesis. 2004;38:195–9.
Ohyama T, Mohamed OA, Taketo MM, Dufort D, Groves AK. Wnt signals mediate a fate decision between otic placode and epidermis. Development. 2006;133:865–75.
Jayasena CS, Ohyama T, Segil N, Groves AK. Notch signaling augments the canonical Wnt pathway to specify the size of the otic placode. Development. 2008;135:2251–61.
Freter S, Muta Y, Mak S-S, Rinkwitz S, Ladher RK. Progressive restriction of otic fate: the role of FGF and Wnt in resolving inner ear potential. Development. 2008;135:3415–24.
Freyer L, Aggarwal V, Morrow BE. Dual embryonic origin of the mammalian otic vesicle forming the inner ear. Development. 2011;138:5403–14.
Ritter KE, Martin DM. Neural crest contributions to the ear: implications for congenital hearing disorders. Hear Res. 2018;376:22–32.
Tchieu J, Zimmer B, Fattahi F, Amin S, Zeltner N, Chen S, et al. A modular platform for differentiation of human PSCs into all major ectodermal lineages. Cell Stem Cell. 2017;21:399–410.
Leung AW, Murdoch B, Salem AF, Prasad MS, Gomez GA, García-Castro MI. WNT/β-catenin signaling mediates human neural crest induction via a pre-neural border intermediate. Development. 2016;143:398–410.
Gomez GA, Prasad MS, Sandhu N, Shelar PB, Leung AW, García-Castro MI. Human neural crest induction by temporal modulation of WNT activation. Dev Biol. 2019;449:99–106.
Lee J, Rabbani CC, Gao H, Steinhart MR, Woodruff BM, Pflum ZE, et al. Hair-bearing human skin generated entirely from pluripotent stem cells. Nature. 2020;582:399–404.
Chauhan BK, Lou M, Zheng Y, Lang RA. Balanced Rac1 and RhoA activities regulate cell shape and drive invagination morphogenesis in epithelia. Proc Natl Acad Sci. 2011;108:18289–94.
Sai X, Ladher RK. Early steps in inner ear development: induction and morphogenesis of the otic placode. Front Pharm. 2015;6:19.
Shyer AE, Rodrigues AR, Schroeder GG, Kassianidou E, Kumar S, Harland RM. Emergent cellular self-organization and mechanosensation initiate follicle pattern in the avian skin. Science. 2017;357:811–5.
Ohta S, Schoenwolf GC. Hearing crosstalk: the molecular conversation orchestrating inner ear dorsoventral patterning. Wiley Interdiscip Rev Dev Biol. 2018;7:e302.
Liang JK, Bok J, Wu DK. Distinct contributions from the hindbrain and mesenchyme to inner ear morphogenesis. Dev Biol. 2010;337:324–34.
Riccomagno MM, Martinu L, Mulheisen M, Wu DK, Epstein DJ. Specification of the mammalian cochlea is dependent on Sonic hedgehog. Gene Dev. 2002;16:2365–78.
Bok J, Bronner-Fraser M, Wu DK. Role of the hindbrain in dorsoventral but not anteroposterior axial specification of the inner ear. Development. 2005;132:2115–24.
Dessaud E, McMahon AP, Briscoe J. Pattern formation in the vertebrate neural tube: a sonic hedgehog morphogen-regulated transcriptional network. Development. 2008;135:2489–503.
Brown AS, Epstein DJ. Otic ablation of smoothened reveals direct and indirect requirements for Hedgehog signaling in inner ear development. Development. 2011;138:3967–76.
Riccomagno MM, Takada S, Epstein DJ. Wnt-dependent regulation of inner ear morphogenesis is balanced by the opposing and supporting roles of Shh. Gene Dev. 2005;19:1612–23.
Żak M, Plagnol V, Daudet N. A gradient of Wnt activity positions the neurosensory domains of the inner ear. Biorxiv 2020. https://doi.org/10.1101/2020.05.04.071035.
Raft S, Groves AK. Segregating neural and mechanosensory fates in the developing ear: patterning, signaling, and transcriptional control. Cell Tissue Res. 2015;359:315–32.
Ohyama T, Basch ML, Mishina Y, Lyons KM, Segil N, Groves AK. BMP signaling is necessary for patterning the sensory and nonsensory regions of the developing mammalian cochlea. J Neurosci. 2010;30:15044–51.
Driver EC, Pryor SP, Hill P, Turner J, Rüther U, Biesecker LG, et al. Hedgehog signaling regulates sensory cell formation and auditory function in mice and humans. J Neurosci. 2008;28:7350–8.
Hayashi T, Ray CA, Bermingham-McDonogh O. Fgf20 is required for sensory epithelial specification in the developing cochlea. J Neurosci. 2008;28:5991–9.
Brooker R, Hozumi K, Lewis J. Notch ligands with contrasting functions: Jagged1 and Delta1 in the mouse inner ear. Development. 2006;133:1277–86.
Pan W, Jin Y, Chen J, Rottier RJ, Steel KP, Kiernan AE. Ectopic expression of activated notch or SOX2 reveals similar and unique roles in the development of the sensory cell progenitors in the mammalian inner ear. J Neurosci. 2013;33:16146–57.
Gnedeva K, Wang X, McGovern M, Barton M, Tao L, Trecek T, et al. Organ of Corti size is governed by Yap/Tead-mediated progenitor self-renewal. Proc Natl Acad Sci. 2020;117:202000175.
Chang W, Nunes FD, Jesus-Escobar JMD, Harland R, Wu DK. Ectopic noggin blocks sensory and nonsensory organ morphogenesis in the chicken inner ear. Dev Biol. 1999;216:369–81.
Chang W, Brigande JV, Fekete DM, Wu DK. The development of semicircular canals in the inner ear: role of FGFs in sensory cristae. Development. 2004;131:4201–11.
Chang W, Lin Z, Kulessa H, Hebert J, Hogan BLM, Wu DK. Bmp4 is essential for the formation of the vestibular apparatus that detects angular head movements. PLoS Genet. 2008;4:e1000050.
Ono K, Sandell LL, Trainor PA, Wu DK. Retinoic acid synthesis and autoregulation mediate zonal patterning of vestibular organs and inner ear morphogenesis. Development. 2020;147:dev192070.
Brown R, Groves AK. Hear for notch: control of cell fates in the inner ear by notch signaling. Biomolecules. 2020;10:370.
Tian C, Johnson KR. TBX1 is required for normal stria vascularis and semicircular canal development. Dev Biol. 2019;457:91–103.
Romand R, Albuisson E, Niederreither K, Fraulob V, Chambon P, Dollé P. Specific expression of the retinoic acid-synthesizing enzyme RALDH2 during mouse inner ear development. Mech Dev. 2001;106:185–9.
Zheng Y, Xue X, Shao Y, Wang S, Esfahani SN, Li Z, et al. Controlled modelling of human epiblast and amnion development using stem cells. Nature. 2019;573:421–5.
Manfrin A, Tabata Y, Paquet ER, Vuaridel AR, Rivest FR, Naef F, et al. Engineered signaling centers for the spatially controlled patterning of human pluripotent stem cells. Nat Methods. 2019;16:640–8.
Schutgens F, Rookmaaker MB, Margaritis T, Rios A, Ammerlaan C, Jansen J, et al. Tubuloids derived from human adult kidney and urine for personalized disease modeling. Nat Biotechnol. 2019;37:303–13.
Brandenberg N, Hoehnel S, Kuttler F, Homicsko K, Ceroni C, Ringel T, et al. High-throughput automated organoid culture via stem-cell aggregation in microcavity arrays. Nat Biomed Eng. 2020;4:863–74.
Yu KS, Frumm SM, Park JS, Lee K, Wong DM, Byrnes L et al. Development of the mouse and human cochlea at single cell resolution. Biorxiv 2019. https://doi.org/10.1101/739680.
Kolla L, Kelly MC, Mann ZF, Anaya-Rocha A, Ellis K, Lemons A, et al. Characterization of the development of the mouse cochlear epithelium at the single cell level. Nat Commun. 2020;11:2389.
Nishio S, Hattori M, Moteki H, Tsukada K, Miyagawa M, Naito T, et al. Gene expression profiles of the cochlea and vestibular endorgans. Ann Otol Rhinol Laryngol. 2015;124:6S–48S.
Fukunaga I, Fujimoto A, Hatakeyama K, Aoki T, Nishikawa A, Noda T, et al. In-vitro models of GJB2-related hearing loss recapitulate Ca2+ transients via a gap junction characteristic of developing cochlea. Stem Cell Rep. 2016;7:1023–36.
Zhong C, Chen Z, Luo X, Wang C, Jiang H, Shao J, et al. Barhl1 is required for the differentiation of inner ear hair cell-like cells from mouse embryonic stem cells. Int J Biochem Cell Biol. 2018;96:79–89.
Tang P-C, Alex AL, Nie J, Lee J, Roth AA, Booth KT, et al. Defective Tmprss3-associated hair cell degeneration in inner ear organoids. Stem Cell Rep. 2019;13:147–62.
Chen JR, Tang ZH, Zheng J, Shi HS, Ding J, Qian XD, et al. Effects of genetic correction on the differentiation of hair cell-like cells from iPSCs with MYO15A mutation. Cell Death Differ. 2016;23:1347–57.
Tang Z-H, Chen J-R, Zheng J, Shi H-S, Ding J, Qian X-D, et al. Genetic correction of induced pluripotent stem cells from a deaf patient with MYO7A mutation results in morphologic and functional recovery of the derived hair cell-like cells. Stem Cell Transl Med. 2016;5:561–71.
Hosoya M, Saeki T, Saegusa C, Matsunaga T, Okano H, Fujioka M, et al. Estimating the concentration of therapeutic range using disease-specific iPS cells: low-dose rapamycin therapy for Pendred syndrome. Regen Ther. 2019;10:54–63.
Ding D, Liu H, Qi W, Jiang H, Li Y, Wu X, et al. Ototoxic effects and mechanisms of loop diuretics. J Otol. 2016;11:145–56.
Breglio AM, Rusheen AE, Shide ED, Fernandez KA, Spielbauer KK, McLachlin KM, et al. Cisplatin is retained in the cochlea indefinitely following chemotherapy. Nat Commun. 2017;8:1654.
Khela H, Kenna MA. Genetics of pediatric hearing loss: a functional perspective. Laryngosc. Investig. Otolaryngol. 2020;5:511–9.
Zhang N, Cai J, Xu L, Wang H, Liu W. Cisplatin-induced stria vascularis damage is associated with inflammation and fibrosis. Neural Plast. 2020;2020:1–13.
McInturff S, Burns JC, Kelley MW. Characterization of spatial and temporal development of Type I and Type II hair cells in the mouse utricle using new cell-type-specific markers. Biol Open. 2018;7:bio038083.
Ciuman RR. Stria vascularis and vestibular dark cells: characterisation of main structures responsible for inner-ear homeostasis, and their pathophysiological relations. J Laryngol Otol. 2009;123:151–62.
Jiang H, Wang L, Beier KT, Cepko CL, Fekete DM, Brigande JV. Lineage analysis of the late otocyst stage mouse inner ear by transuterine microinjection of A retroviral vector encoding alkaline phosphatase and an oligonucleotide Library. PLoS ONE. 2013;8:e69314.
Locher H, Groot de JCMJ, Iperen van L, Huisman MA, Frijns JHM, Lopes SMC, et al. Development of the stria vascularis and potassium regulation in the human fetal cochlea: Insights into hereditary sensorineural hearing loss. Dev Neurobiol. 2015;75:1219–40.
Trowe M-O, Maier H, Petry M, Schweizer M, Schuster-Gossler K, Kispert A. Impaired stria vascularis integrity upon loss of E-cadherin in basal cells. Dev Biol. 2011;359:95–107.
Beelen van ESA, Valk van der WH, Groot de JCMJ, Hensen EF, Locher H, Benthem van PPG. Migration and fate of vestibular melanocytes during development of the human inner ear. Dev Neurobiol. 2020. https://doi.org/10.1002/dneu.22786.
Wangemann P. Supporting sensory transduction: cochlear fluid homeostasis and the endocochlear potential. J Physiol. 2006;576:11–21.
Furness DN. Forgotten fibrocytes: a neglected, supporting cell type of the cochlea with the potential to be an alternative therapeutic target in hearing loss. Front Cell Neurosci. 2019;13:532.
Royaux IE, Belyantseva IA, Wu T, Kachar B, Everett LA, Marcus DC, et al. Localization and functional studies of pendrin in the mouse inner ear provide insight about the etiology of deafness in pendred syndrome. J Assoc Res Otolaryngol. 2003;4:394–404.
Locher H, Frijns JH, Iperen van L, Groot de JC, Huisman MA, Lopes SMC, et al. Neurosensory development and cell fate determination in the human cochlea. Neural Dev. 2013;8:20.
Valero MD, Burton JA, Hauser SN, Hackett TA, Ramachandran R, Liberman MC. Noise-induced cochlear synaptopathy in rhesus monkeys (Macaca mulatta). Hear. Res. 2017;353:213–23.
Kupari J, Häring M, Agirre E, Castelo-Branco G, Ernfors P. An atlas of vagal sensory neurons and their molecular specialization. Cell Rep. 2019;27:2508–e4.
Gatto G, Smith KM, Ross SE, Goulding M. Neuronal diversity in the somatosensory system: bridging the gap between cell type and function. Curr Opin Neurobiol. 2019;56:167–74.
Sharma N, Flaherty K, Lezgiyeva K, Wagner DE, Klein AM, Ginty DD. The emergence of transcriptional identity in somatosensory neurons. Nature. 2020;577:392–8.
Tritsch NX, Yi E, Gale JE, Glowatzki E, Bergles DE. The origin of spontaneous activity in the developing auditory system. Nature. 2007;450:50–55.
Shrestha BR, Chia C, Wu L, Kujawa SG, Liberman MC, Goodrich LV. Sensory neuron diversity in the inner ear is shaped by activity. Cell. 2018;174:1229–e17.
Sun S, Babola T, Pregernig G, So KS, Nguyen M, Su S-SM, et al. Hair cell mechanotransduction regulates spontaneous activity and spiral ganglion subtype specification in the auditory system. Cell. 2018;174:1247–e15.
Butts JC, McCreedy DA, Martinez-Vargas JA, Mendoza-Camacho FN, Hookway TA, Gifford CA, et al. Differentiation of V2a interneurons from human pluripotent stem cells. Proc Natl Acad Sci. 2017;114:4969–74.
Birey F, Andersen J, Makinson CD, Islam S, Wei W, Huber N, et al. Assembly of functionally integrated human forebrain spheroids. Nature. 2017;545:54–59.
Okaty BW, Sturrock N, Lozoya YE, Chang Y, Lyon KA, Alekseyenko OV et al. A single-cell transcriptomic and anatomic atlas of mouse dorsal raphe Pet1 neurons. Biorxiv 2020. https://doi.org/10.1101/2020.01.28.923375.
Coate TM, Raft S, Zhao X, Ryan AK, Crenshaw EB, Kelley MW. Otic mesenchyme cells regulate spiral ganglion axon fasciculation through a Pou3f4/EphA4 signaling pathway. Neuron. 2012;73:49–63.
Braunstein EM, III EBC, Morrow BE, Adams JC. Cooperative function of Tbx1 and Brn4 in the periotic mesenchyme is necessary for cochlea formation. J Assoc Res Otolaryngol. 2008;9:33–43.
Trowe M-O, Maier H, Schweizer M, Kispert A. Deafness in mice lacking the T-box transcription factor Tbx18 in otic fibrocytes. Development. 2008;135:1725–34.
Parzefall T, Shivatzki S, Lenz DR, Rathkolb B, Ushakov K, Karfunkel D, et al. Cytoplasmic mislocalization of POU3F4 due to novel mutations leads to deafness in humans and mice. Hum Mutat. 2013;34:1102–10.
Song MH, Choi S-Y, Wu L, Oh S-K, Lee HK, Lee DJ, et al. Pou3f4 deficiency causes defects in otic fibrocytes and stria vascularis by different mechanisms. Biochem Biochem Biophys Res Commun. 2011;404:528–33.
Taguchi A, Nishinakamura R. Higher-order kidney organogenesis from pluripotent stem cells. Cell Stem Cell. 2017;21:730–.e6.
Sharma A, Sances S, Workman MJ, Svendsen CN. Multi-lineage human iPSC-derived platforms for disease modeling and drug discovery. Cell Stem Cell. 2020;26:309–29.
Han L, Chaturvedi P, Kishimoto K, Koike H, Nasr T, Iwasawa K, et al. Single cell transcriptomics identifies a signaling network coordinating endoderm and mesoderm diversification during foregut organogenesis. Nat Commun. 2020;11:4158.
Nyberg S, Abbott NJ, Shi X, Steyger PS, Dabdoub A. Delivery of therapeutics to the inner ear: the challenge of the blood-labyrinth barrier. Sci Transl Med. 2019;11:eaao0935.
Shi X. Physiopathology of the cochlear microcirculation. Hear Res. 2011;282:10–24.
Nzou G, Wicks RT, Wicks EE, Seale SA, Sane CH, Chen A, et al. Human cortex spheroid with a functional blood brain barrier for high-throughput neurotoxicity screening and disease modeling. Sci Rep. 2018;8:7413.
Wimmer RA, Leopoldi A, Aichinger M, Wick N, Hantusch B, Novatchkova M, et al. Human blood vessel organoids as a model of diabetic vasculopathy. Nature. 2019;565:505–10.
Nzou G, Wicks RT, VanOstrand NR, Mekky GA, Seale SA, EL-Taibany A, et al. Multicellular 3D Neurovascular unit model for assessing hypoxia and neuroinflammation induced blood-brain barrier dysfunction. Sci Rep. 2020;10:9766.
Homan KA, Gupta N, Kroll KT, Kolesky DB, Skylar-Scott M, Miyoshi T, et al. Flow-enhanced vascularization and maturation of kidney organoids in vitro. Nat Methods. 2019;16:255–62.
Cakir B, Xiang Y, Tanaka Y, Kural MH, Parent M, Kang Y-J, et al. Engineering of human brain organoids with a functional vascular-like system. Nat Methods. 2019;16:1169–75.
Palikuqi B, Nguyen D-HT, Li G, Schreiner R, Pellegata AF, Liu Y, et al. Adaptable haemodynamic endothelial cells for organogenesis and tumorigenesis. Nature. 2020;585:426–32.
Nakanishi H, Prakash P, Ito T, Kim HJ, Brewer CC, Harrow D, et al. Genetic hearing loss associated with autoinflammation. Front Neurol. 2020;11:141.
Ormel PR, Sá de RV, Bodegraven van EJ, Karst H, Harschnitz O, Sneeboer MAM, et al. Microglia innately develop within cerebral organoids. Nat Commun. 2018;9:4167.
Schmunk G, Kim CN, Soliman SS, Keefe MG, Bogdanoff D, Tejera D et al. Human microglia upregulate cytokine signatures and accelerate maturation of neural networks. Biorxiv 2020. https://doi.org/10.1101/2020.03.24.006874.
Lopez-Yrigoyen M, May A, Ventura T, Taylor H, Fidanza A, Cassetta L et al. Production and characterization of human macrophages from pluripotent stem cells. J Vis Exp. 2020. https://doi.org/10.3791/61038.
Burns JC, Kelly MC, Hoa M, Morell RJ, Kelley MW. Single-cell RNA-Seq resolves cellular complexity in sensory organs from the neonatal inner ear. Nat Commun. 2015;6:8557–8557.
Ellwanger DC, Scheibinger M, Dumont RA, Barr-Gillespie PG, Heller S. Transcriptional dynamics of hair-bundle morphogenesis revealed with cell trails. Cell Rep. 2018;23:2901–e14.
Korrapati S, Taukulis I, Olszewski R, Pyle M, Gu S, Singh R, et al. Single cell and single nucleus RNA-Seq reveal cellular heterogeneity and homeostatic regulatory networks in adult mouse stria vascularis. Front Mol Neurosci. 2019;12:316.
Hoa M, Olszewski R, Li X, Taukulis I, Gu S, DeTorres A, et al. Characterizing adult cochlear supporting cell transcriptional diversity using single-cell RNA-Seq: validation in the adult mouse and translational implications for the adult human cochlea. Front Mol Neurosci. 2020;13:13.
Popescu D-M, Botting RA, Stephenson E, Green K, Webb S, Jardine L, et al. Decoding human fetal liver haematopoiesis. Nature. 2019;574:365–71.
We acknowledge all our colleagues whose important work could not be cited due to space limitations. We would like to thank C. Nist-Lund, J. Lee, C. Deakin, and S. Serdy for critical comments on the manuscript. This work was supported by grants from the National Institute of Health (grants R01DC017461 and R03DC015624 to KRK, and F30DC018715 to MRS) and the Department of Defense (grant NF180102 to KRK).
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The authors declare that they have no conflict of interest.
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van der Valk, W.H., Steinhart, M.R., Zhang, J. et al. Building inner ears: recent advances and future challenges for in vitro organoid systems. Cell Death Differ 28, 24–34 (2021). https://doi.org/10.1038/s41418-020-00678-8
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