Fig. 1 | Cell Death & Differentiation

Fig. 1

From: A functional genetic screen defines the AKT-induced senescence signaling network

Fig. 1

Transcriptomic and metabolic profiling identify signatures of AIS. a–d BJ-TERT cells were transduced with pBabe empty vector control, myrAKT1, or HRASV12 and analyzed at 14 dpt. a Cells were stained for SA-ßGal activity or EdU. DAPI staining was used to visualize nuclei. Scale bars = 50 μm. b, c Quantification of percentage of cells with positive staining for b SA-ßGal activity or c EdU. d Western blots showing senescence markers as well as phosphorylated and total ERK. GAPDH was probed as a loading control. e Differentially expressed genes (FC ≤ −1.5 or FC ≥ 1.5, FDR ≤ 0.01) between cells undergoing AIS versus proliferating cells, which are downregulated (blue), upregulated (red), or not significant (black). The top 20 significantly upregulated and downregulated genes are indicated. f Heatmap from RNA-seq data of AIS versus proliferating cells showing upregulation and FC of inhibitors of RAS/ERK signaling. n = 3 biological replicates. g Gene set enrichment analysis showing normalized enrichment score (NES) for significantly upregulated and downregulated gene sets during AIS or OIS compared to proliferating cells. h Hierarchical clustering of the top 50 significant metabolites in proliferating cells or those undergoing AIS or OIS. Note: The sample AIS-1 was excluded due to a technical issue in sample processing independent of sample quality

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