Fig. 3 | Cell Death & Differentiation

Fig. 3

From: UBAP2L arginine methylation by PRMT1 modulates stress granule assembly

Fig. 3

UBAP2L affects G3BP2 association with SG components. a UBAP2L expression under stress or recovery conditions. HeLa cells were treated with or relieved from the indicated stresses, and then subjected to WB analysis of UBAP2L, its binding partners, and other indicated SG proteins. b Expression of the UBAP2L binding partners in stressed HeLa cells after UBAP2L knockdown. (c) Expression of the UBAP2L binding partners in stressed HeLa cells after UBAP2L overexpression. d Co-IP/WB analysis of the associations of G3BP2 with PABPC1 and RPS6 after UBAP2L knockdown. HeLa cells were simultaneously transfected with the HA tag or HA-G3BP2 plasmid with/without UBAP2L-siRNA, followed by treatment with/without AS (500 μM, 1 h), in combination with/without RNase A in the lysis buffer. The HA precipitates were analyzed by WB for PABPC1 and RPS6

Back to article page