Fig. 5 | Cell Death & Differentiation

Fig. 5

From: Protection of ZIKV infection-induced neuropathy by abrogation of acute antiviral response in human neural progenitors

Fig. 5

Activation of ISGs by IFNβ administration causes NPC growth arrest. a qRT-PCR analysis of ISG mRNA expression of FD organoids treated with 40 ng/ml IFNβ or IFNγ or untreated control for 6 days. Data are presented as mean ± SEM. n=3. Unpaired two-tailed Student’s t-test. *p < 0.05, **p < 0.01 compare to untreated controls. b Size of FD organoids treated with or without 40 ng/ml IFNβ for 0, 2 and 6 days. Data are presented as mean ± SEM. n = 200 from three independent experiments. Unpaired two-tailed Student’s t-test. **p < 0.01 compared to the untreated controls. ce Confocal images stained with Ki67, BrdU or pH3 in red and Hoechst nuclei staining in blue of untreated or 40 ng/ml IFNβ−treated FD organoids for 3 days. Scale bar, 100 μm. f Percentage of Ki67, BrdU and pH3 positive cells. Data are presented as mean ± SEM. n = 3. Unpaired two-tailed Student’s t-test. *p < 0.05 compared to untreated controls