Fig. 2 | Cell Death & Differentiation

Fig. 2

From: Protection of ZIKV infection-induced neuropathy by abrogation of acute antiviral response in human neural progenitors

Fig. 2

ZIKV abrogates growth of hESC-derived brain organoids of different regional identities. a Light images of FD, FV, H&S organoids exposed to mock conditions or ZIKV at MOI =0.5 at 1, 3 or 6 dpi. b Size of FD, FV and H&S organoids exposed to mock or ZIKV at MOI = 0.5 at 1, 3 or 6 dpi. Quantification data of organoids diameters are presented as mean ± SEM. n = 200 from three independent experiments. Unpaired two-tailed Student’s t-test. ****p < 0.0001 compared to mock controls. c Numbers of FD, FV and H&S organoids remaining after mock or ZIKV infection at MOI = 0.5 at 1, 3 or 6 dpi. Quantification data are presented as mean ± SEM. n = 3. Unpaired two-tailed Student’s t-test. ****p < 0.0001 compared to mock controls. d Confocal images stained with ZIKV envelope proteins in green, or BrdU, Ki67 and pH3 in red of mock or ZIKV-infected FD, FV, H&S organoids. Scale bar, 100 μm. eg Quantitative analyses of percentage of BrdU, Ki67 and pH3 positive cells in mock or ZIKV groups. Data are presented as mean ± SEM. n = 3. Unpaired two-tailed Student’s t-test. *p < 0.05, **p < 0.01 compared to corresponding mock controls

Back to article page