Fig. 6 | Cell Death & Differentiation

Fig. 6

From: Diabetes induces the activation of pro-ageing miR-34a in the heart, but has differential effects on cardiomyocytes and cardiac progenitor cells

Fig. 6

miR-34a activity inhibition reduces apoptotic cell death in high glucose treated human cardiomyocytes. a, b Representative immunoblot images and quantitative bar graphs showing the expression of SIRT-1 a and p53 b in AC-16 cardiomyocytes following inhibition of miR-34a (anti-miR-34a) activity. β-actin was used as internal control. Data are represented as fold changes to cardiomyocytes cultured in normal glucose (NG) and are transfected with Scrambled sequence (Scr). Values are mean ± SEM. c Quantitative bar graphs showing the caspase-3/7 activity in AC-16 following inhibition of miR-34a (anti-miR-34a) activity. Data are represented as relative luciferase units and are mean ± SEM. *P < 0.05 and ****P < 0.0001 vs. NG-Scr cells; # P < 0.05, ### P < 0.001, and #### P < 0.0001 vs. corresponding Scrambled sequence (Scr) transfected cells. n = 3 for all the experiments

Back to article page