Fig. 5 | Cell Death & Differentiation

Fig. 5

From: Diabetes induces the activation of pro-ageing miR-34a in the heart, but has differential effects on cardiomyocytes and cardiac progenitor cells

Fig. 5

Diabetes activates miR-34a and increased apoptosis in human diabetic CPCs. a Quantitative box plots showing the expression level of surface antigens CD34, CD90, and CD105 in human CPCs by flow cytometry analysis. Data are represented as percentage (%) of positive cells. n = 14 in each group. b Representative immunofluorescence images and quantitative bar graphs showing the expression cardiac markers in differentiated CPCs. Data are represented as percentage (%) of positive cells. n = 4 in each group. c Quantitative scatter plots showing the differential expression of miR-34a by quantitative RT-PCR analysis in human CPCs. Data are represented as log-fold change in miR-34a expression vs. non-diabetic CPCs and are mean ± SEM. n = 14 in each group. d, e Immunoblot images and quantitative bar graphs showing the expression of SIRT-1 d and p53 e in diabetic and non-diabetic CPCs. β-actin was used as internal control. Data are represented as fold changes to non-diabetic CPCs and are mean ± SEM. n = 6 in each group. f Quantitative bar graphs showing the caspase-3/7 activity in NG and HG cultured cells. Data are represented as relative luciferase units and are mean ± SEM. *P < 0.05, **P < 0.01, and ****P < 0.0001 vs. non-diabetic CPCs. n = 6 individual CPC samples in each group

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