Abstract
As part of the DNA damage response (DDR) network, the tumour suppressor Breast cancer susceptibility gene 1 (BRCA1) is activated to facilitate DNA repair, transcription and cell cycle control. BRC-1, the Caenorhabditis elegans ortholog of BRCA1, has conserved function in DNA double strand break repair, wherein a loss of brc-1 results in high levels of germline apoptosis. BRAP2/IMP was initially identified as a BRCA1 associated binding protein and previously we have shown that the C. elegans brap-2 deletion mutant experiences BRC-1 dependent larval arrest when exposed to low concentrations of paraquat. Since BRC-1 function in the germline is conserved, we wanted to determine the role of BRAP-2 in DNA damage induced germline apoptosis in C. elegans. We examined levels of germ cell death following DNA damage and found that brap-2(ok1492) mutants display reduced levels of germline apoptosis when compared to the wild type, and the loss of brap-2 significantly reduced germ cell death in brc-1 mutant animals. We also found increased mRNA levels of skn-1 following DNA damage in brap-2 mutants and that skn-1 RNAi knockdown in brap-2;brc-1 double mutants and a loss of pmk-1 mutation in brap-2 mutants increased apoptosis to wild type levels, indicating that brap-2 promotion of cell survival requires PMK-1 and SKN-1. Since mammalian BRAP2 has been shown to bind the AKT phosphatase PHLPP1/2, it suggests that BRAP2 could be involved in the Insulin/Insulin-like growth factor Signaling (IIS) pathway. We found that this interaction is conserved between the C. elegans homologs and that a loss of akt-1 in brap-2 mutants increased germline apoptosis. Thus in response to DNA damage, our findings suggest that BRAP-2 is required to attenuate the pro-cell survival signals of AKT-1 and PMK-1/SKN-1 to promote DNA damage induced germline apoptosis.
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Introduction
Activation of apoptosis is an essential process in development and ensures the elimination of abnormal cells that can lead to tumour formation and the onset of cancer [1,2,3]. In Caenorhabditis elegans, apoptosis is genetically conserved and functions in the germline to provide cytoplasmic contents and maternal determinants to growing oocytes, as well as remove cells whose DNA has been damaged beyond repair by exogenous stressors such as ionizing radiation (IR), chemicals and toxins [4].
Apoptosis activated by the p53-like protein CEP-1 occurs in the pachytene region of the germline, known as the “death loop” [4]. DNA damage leads to the activation of CEP-1 by upstream DNA damage sensors that phosphorylate and activate CEP-1 [5,6,7]. CEP-1 transcriptional upregulation of the genes for pro-apoptotic factors EGL-1 and CED-13 release CED-9 inhibition of CED-4 to activate CED-3 for the execution of apoptosis [6]. BRC-1 has been reported to facilitate the conserved repair of DNA double strand breaks (DSBs) using homologous recombination through inter-sister recombination [1]. brc-1 mutants exhibit increased levels of germline apoptosis, increased embryonic lethality and X-chromosome non-disjunction [8].
BRAP2 (BRCA1-binding protein 2 or BRAP as listed in the HUGO database) in mammalian systems has been characterized as a RAS-responsive effector protein capable of ubiquitin ligase activity [9]. BRAP2 was first found to bind to the nuclear localization signal (NLS) motifs of BRCA1 and retain BRCA1 in the cytoplasm, preventing its nuclear localization [10]. Recently, BRAP2 has been found to bind to and act as a scaffold protein for PHLPP1, an AKT protein phosphatase, in mouse testes [11]. In C. elegans, BRAP2 is conserved and known as BRAP-2 [2]. When exposed to oxidative stress, brap-2 mutants experience lethality and L1 developmental arrest due to high levels of CKI-1 (p21 ortholog) [2]. Loss of BRC-1 in brap-2 mutants prevented lethality, developmental arrest and decreased CKI-1 levels, establishing a genetic link between BRAP-2 and BRC-1.
Due to the conservation of BRC-1 function in C. elegans and the genetic interaction between BRC-1 and BRAP-2 upon oxidative stress, we examined the role of BRAP-2 in DNA damage induced germline apoptosis. We found that brap-2(ok1492) mutants exhibit a reduction in DNA damage induced germline apoptosis and that germline apoptosis caused by a loss of BRC-1 requires BRAP-2. We determined that brap-2 mutants are dauer defective and the interaction between PHLP-2 (C. elegans homolog of AKT protein phosphatase PHLPP1/2) and BRAP-2 is conserved in C. elegans. We also found that a loss of PMK-1, SKN-1 and AKT-1 in brap-2 mutants increases apoptosis. Taken together, these observations suggest a model where a loss of BRAP-2 limits DNA damage induced germline apoptosis and promotes cell survival through regulation of the PMK-1 activated SKN-1/Nrf2 oxidative stress response pathway and the IIS Pathway.
Results
BRAP-2 is required for DNA damage induced germline apoptosis
Since BRC-1 functions in the DDR, and having previously established a genetic link between BRC-1 and BRAP-2 during oxidative stress, we wanted to determine whether BRAP-2 is necessary for the induction of germline apoptosis. Unlike brc-1 mutants, we observed a decrease in apoptosis in brap-2(ok1492) mutants compared to the wild type following DNA damage (Fig. 1a). When we introduced the ced-1p::GFP reporter strain into brap-2(ok1492), allowing for both early and late phase apoptotic cells to be detected [12], a 50% reduction in corpse appearance following DNA damage was observed (Fig. S1). However, physiological levels of apoptosis in brap-2(ok1492) mutants are similar to the wild type (Fig. 1b), indicating that brap-2 may specifically regulate DNA damage induced germline apoptosis.
To test if brap-2 is required for the high levels of germline apoptosis in brc-1 mutants, we generated a brap-2(ok1492);brc-1(tm1145) double mutant and scored apoptotic levels following IR. We observed a significant reduction in germ cell death at both 60 and 120 Gy (Fig. 1b). When both wild type and brc-1(tm1145) mutants were fed brap-2 RNAi, a similar 2.5-fold reduction in apoptosis was observed (Fig. 1c). These results indicate that excessive apoptosis caused by a loss of brc-1 is dependent on brap-2. The brap-2(ok1492) mutation does not affect the timing or kinetics of corpse appearance, since 48 h after DNA damage exposure, brap-2(ok1492) mutants displayed low levels of apoptosis compared to the wild type (Fig. S2).
We determined the expression of BRAP-2 by generating a BRAP-2::GFP strain using CRISPR-Cas9 and found that BRAP-2 is highly expressed in the germline (Fig. S3). To ensure that reduced apoptosis in brap-2 mutants was not caused by a decrease in germ cell number, we scored the number of nuclei before and after DNA damage using whole worm DAPI staining [13] and found that the total number of germline nuclei (nuclei per µm2) in brap-2(ok1492) mutants did not differ significantly compared to the wild type (Fig. S4A). Physiologically, we found that brap-2 mutants experienced a 20% reduction in total germline nuclei compared to the wild type, which we believe does not account for the consistent 50% reduction in apoptosis seen in brap-2 mutants following DNA damage (Fig. S4B). In order to determine whether BRAP-2 is involved in the regulation of apoptosis of somatic cells, we scored the number of apoptotic cells in developing embryos directly, using a ced-1p::GFP reporter strain. We found that in 5 distinct stages of embryonic development, brap-2(ok1492) mutants displayed no significant difference in the number of corpses when compared to the wild type (Fig. S4C).
The 9-1-1 checkpoint (MRT-2, HUS-1, CLK-2) is a complex of protein sensors that interpret DNA damage signals and relay them to induce mitotic arrest in an attempt to repair DNA [5]. If the damage is beyond repair they send signals to ATM-1 and ATL-1 kinases that can activate CEP-1 (p53 ortholog) for the induction of apoptosis [5,14]. In the C. elegans germline, nuclei arrested in the mitotic zone can be viewed directly, enlarging following DNA damage to enter G2 phase cell cycle arrest [13]. Interestingly, brap-2(ok1492) mutants displayed an increase in mitotically arrested nuclei physiologically, and a more robust proportion of arrested nuclei following IR exposure (Fig. 2). Therefore, a loss of BRAP-2 does not (i) alter the timing of corpse appearance, (ii) inhibit germline proliferation, (iii) or prevent mitotic arrest, suggesting that BRAP-2 specifically promotes DNA damage induced germline apoptosis and is required for a brc-1 mutant triggered activation of apoptosis.
DNA damage induced germline apoptosis is activated by a conserved, canonical signaling pathway initiated when CEP-1 up-regulates egl-1 and ced-13 (BH3 orthologs) transcription [15,16,17]. We asked whether a loss of brap-2 affects CEP-1 function by monitoring expression of egl-1 and ced-13. Surprisingly, brap-2 and brap-2;brc-1 double mutants displayed a significant increase in egl-1 and ced-13 mRNA expression levels both prior to and following DNA damage induction (Fig. 3a, b), indicating that the decrease in apoptosis seen in brap-2(ok1492) mutants is not due to reduced activity of the canonical apoptotic pathway. brap-2 mutants also displayed elevated mRNA levels of ced-9 following DNA damage (Fig. 3c). CED-9 is a “pro-survival” gene that prevents apoptosis and its ablation leads to immense germ cell death and inviability [18]. To investigate the potential interaction between BRAP-2 and CED-9, we generated brap-2;ced-9 double mutants and assessed apoptotic levels and found that the absence of brap-2 in ced-9 mutants failed to reduce the high levels of apoptosis seen in ced-9 mutants (Fig. 4a), indicating that decrease in apoptosis seen in brap-2 mutants probably requires CED-9.
SKN-1 and PMK-1 are required to decrease apoptosis in brap-2;brc-1 mutants
SKN-1 is essential for the initiation of gene expression against harmful reactive oxygen species (ROS) [19,20]. Previously, we found that brap-2 mutants increased SKN-1 activity, promoting expression of its target phase II detoxification genes [21]. We wanted to determine whether SKN-1 is responsible for the brap-2(ok1492) effect on germline apoptosis. While we previously found that skn-1 mRNA levels are increased in brap-2 mutants [21], here we found that brap-2 mutants experience a 2-fold increase in skn-1c mRNA levels after IR (Fig S5). We then asked whether the reduction of apoptosis in brap-2 mutants was dependent on SKN-1. Using RNAi, we knocked down skn-1 in brap-2 mutants and scored germline apoptosis before and after IR. We observed that skn-1 RNAi knockdown in wild type worms does not affect apoptosis, as previously reported [22] and that skn-1 RNAi knockdown increased apoptosis in brap-2;brc-1 double mutants to levels similar to the wild type (Fig. 4b). pmk-1 RNAi in brap-2;brc-1 double mutants produced a similar increase in levels of apoptosis (Fig. S5C). However, we did not observe increased apoptosis in single brap-2 mutants with skn-1 knockdown. This suggests that a loss of brap-2 promotes cell survival in brc-1 mutants, in part, through SKN-1 activation by PMK-1.
Phosphorylation of SKN-1 by PMK-1 (p38 MAPK ortholog) and MPK-1 (ERK1/2 ortholog) promote SKN-1 entry into intestinal nuclei and activation of its target genes [23,24]. We asked if PMK-1 and MPK-1 contribute to the reduced apoptosis in brap-2 mutants following DNA damage exposure. We scored apoptotic levels in brap-2;pmk-1 double mutants and found that a loss of pmk-1 in brap-2(ok1492) mutants elevated apoptosis to wild type levels (Fig. 4c). In brap-2(ok1492) mutants, we detected increased endogenous levels of both the non-phosphorylated and phosphorylated forms of ERK1/2 (MPK-1 homolog) (Fig. S6). However, the loss of MPK-1 did not elevate apoptosis in brap-2 mutants (Fig. S7A). In addition, increased mRNA levels of egl-1, ced-13 and ced-9 in brap-2 mutants were not dependent on PMK-1 (Fig. S7B,C). Together, these results suggest that the reduction in apoptosis in brap-2(ok1492) mutants is dependent on PMK-1 activity and indicates that reduced levels of apoptosis previously seen in brap-2;brc-1 mutants, may be mediated in part by SKN-1 through PMK-1.
brap-2(ok1492) mutants are dauer defective
While maintaining our brap-2(ok1492) strain, we observed that brap-2(ok1492) mutants did not appear to enter diapause when starved. Developmental arrest (dauer) is initiated by inactivation of IIS leading to inhibition of AKT-1 and AKT-2 (AKT orthologs), allowing DAF-16 (FOXO ortholog) to enter the nucleus for the transcriptional upregulation of pro-survival and dauer genes [25]. To confirm that brap-2(ok1492) worms fail to enter dauer, we performed a dauer defective assay [26], where following starvation dauer worms were treated with 1% Sodium Dodecyl Sulfate (SDS). We found that similar to daf-16(mu86) mutants, brap-2(ok1492) mutants were unable to survive SDS treatment (Table 1), indicating that the brap-2(ok1492) mutation may inhibit DAF-16, preventing worms from entering dauer. We also tested the effect of removing MPK-1, PMK-1 and AKT-1, and found that they rescued brap-2 mutants and allowed them to enter dauer (Table 1). Interestingly, unlike AKT-1, a loss of AKT-2 in brap-2 mutants did not restore dauer entry. This suggests that BRAP-2 may function specifically through AKT-1 to inhibit DAF-16.
Since brap-2 mutants are dauer defective, we hypothesized that IIS may be enhanced, specifically AKT-1, to decrease DAF-16 activity. To verify whether a loss of brap-2 causes increased IIS signaling, we measured the mRNA expression levels of two known targets of this pathway, ins-7 and ctl-1 [27,28]. Similar to daf-16 mutants, brap-2 mutants displayed a 2-fold increase in ins-7 expression while ctl-1 was significantly reduced (Fig. S8).
We also examined the localization of DAF-16 in vivo, using a DAF-16::GFP reporter strain in a brap-2(ok1492) mutant background. As a positive control for DAF-16 nuclear accumulation, worms were treated with 5 mM sodium arsenite. We found that both before and following arsenite treatment, brap-2(ok1492) mutants displayed a reduction in nuclear accumulation of DAF-16 (Fig. S9). We also saw a modest increase in cytoplasmic DAF-16 localization in brap-2 mutants following 60 Gy of IR. Taken together these results suggest that BRAP-2 regulates IIS, where a loss of BRAP-2 may lead to increased DAF-16 inhibition by AKT-1, both physiologically and when exposed to exogenous stressors.
Loss of akt-1 increased apoptosis in brap-2 mutants
akt-1 and akt-2 gain of function mutants have been found to suppress DNA damage induced germline apoptosis [29]. Having observed that brap-2 mutants inhibit DAF-16 function, we hypothesized that BRAP-2 may function through AKT-1 to regulate DNA damage induced germline apoptosis. We generated a akt-1(ok525);brap-2(ok1492) double mutant strain and scored apoptosis following IR. We found that a loss of akt-1 in brap-2 mutants increased apoptosis to wild type levels (Fig. 5a). Interestingly, although a loss of akt-2 in brap-2 mutants increased apoptosis, it was not as significant as a loss of akt-1. Knockdown of akt-1 using RNAi in both brap-2 and brap-2;brc-1 double mutants also increased apoptosis to wild type levels following DNA damage (Fig. 5b).
We quantified akt-1 mRNA expression in brap-2 mutants and found that akt-1 levels in brap-2 mutants increased 2.2-fold (Fig. S10A). We also found that the level of endogenous AKT-1 in brap-2 mutants was increased 1.43-fold, while phlp-2 mutants produced no change in AKT-1 (Fig. S10B). We then asked if activation of DAF-16 upon removal of akt-1 was responsible for the brap-2 mutant reduction in apoptosis; however, the removal of DAF-16 did not increase apoptosis in brap-2(ok1492) mutants indicating that DAF-16 is not required (Fig. 5a). Together, these results indicate that AKT-1 is at least partially required for the decrease in germline apoptosis seen in brap-2 mutants upon IR, in a daf-16 independent manner.
BRAP-2 interacts with PHLP-2, the C. elegans homolog of the AKT phosphatase PHLPP1/2
BRAP2 is expressed in many tissues, but has been found most enriched in testes [11]. A yeast 2-hybrid screen using a testes specific cDNA library uncovered several BRAP2 binding partners and one candidate was the AKT protein phosphatase PHLPP1. PHLPP1 dephosphorylates AKT at S473 [30,31,32] and inactivates it, preventing the inhibition of FOXO and other targets which promote cell death [33]. C. elegans contains PHLP-2 (F43C1.1), which is orthologous to PHLPP1 and PHLPP2. We found that BRAP-2 physically binds to PHLP-2 in vitro, indicating that this interaction is conserved (Fig. 6a). Therefore, it is possible that BRAP-2 may function to regulate the activation of the IIS/AKT pathway in C. elegans.
PHLP-2 is required to promote apoptosis
Dephosphorylation of AKT by PHLPP1 inactivates AKT, preventing downstream AKT function that leads to cell survival, growth, and proliferation [32]. We hypothesized that loss of PHLP-2 would allow for persistent AKT-1 activation and cause a reduction in germline apoptosis following DNA damage exposure. Physiologically, apoptotic levels in phlp-2(tm7788) mutants was lower than the wild type, and following DNA damage phlp-2 mutants displayed a 4.5-fold reduction in apoptosis (Fig. 6b), indicating PHLP-2 may be required for the general activation of apoptosis. We also found that phlp-2 RNAi knockdown reduced apoptosis in brc-1 mutants (Fig. 6c). Surprisingly, when we generated the akt-1(ok525);phlp-2(tm7788) double mutant strain, we observed a significant reduction in apoptosis following exposure to IR. This indicates PHLP-2 may be required to regulate other proteins besides AKT-1 to promote germline apoptosis (Fig. 6b).
Discussion
Here we provide evidence of a role for BRAP-2 in the DDR, where it is required to promote DNA damage induced germline apoptosis and the high levels of apoptosis incurred by loss of brc-1. While BRC-1 functions to prevent apoptosis, we found that BRAP-2 promotes apoptosis in the germline upon DNA damage. Previously, we found that brc-1 was necessary in somatic cells to induce cell cycle arrest in brap-2 mutants upon oxidative stress [2], demonstrating a different response than in the germline. In contrast to somatic cells which suppress apoptosis and the DDR checkpoint, the germline employs the 9-1-1 DNA damage sensor complex and CEP-1 function to activate cell death in response to DNA damage [34]. Similar to germline cells, somatic cells still express DNA repair genes like BRC-1 to repair DNA damage yet DDR checkpoint signaling is not induced, avoiding apoptosis activation [13,34]. Due to the lack of DDR checkpoint and apoptosis activation in soma compared to the germline, it is possible that the differences in signaling that occur upon DNA damage in these two tissues elicits the activation of diverse downstream effectors to activate distinct responses by BRC-1 and BRAP-2.
Previously, we found that the brap-2(ok1492) mutation increased both SKN-1 localization into intestinal nuclei and expression of phase II detoxification genes in C. elegans [21], revealing BRAP-2 as a negative regulator of SKN-1, whose function is the upregulation of phase II genes for protection against ROS [21]. Although, akt-1 RNAi has been shown to increase SKN-1 nuclear localization [35], our findings indicate that a loss of akt-1 does not affect SKN-1 activity in brap-2 mutants, as increased levels of SKN-1 target genes were not affected by a loss of akt-1 (Fig. S5D,E).
IR can harm cells directly by inducing DSBs and indirectly through “radiolysis”, the hydrolysis of water molecules, leading to the production of ROS and the possible disruption of nucleic acids, proteins and lipids [35]. Similar to its mammalian homolog Nrf2 [36], SKN-1’s cytoprotective role can be inferred by its maintainenance of cellular homeostasis in response to oxidative stress. While repressed SKN-1 mRNA transcripts have been identified within the germline, SKN-1 protein has only been detected somatically [20,37]. It is possible that SKN-1 has a cell survival role in the germline apart from responding to ROS in the soma similar to Nrf2, which has been shown to possess a pro-survival role in cancer through its upregulation of anti-apoptotic genes, promotion of an unaltered metabolism and changes to ubiquitin-proteasome protein turnover [36]. In C. elegans, SKN-1 activity may enhance cell survival in the germline through a “soma to germline interaction”, a relationship characterized previously in the cell non-autonomous regulation of apoptosis [38], lipid regulation [39], germline proliferation [40] and lifespan extension [41].
AKT-1 and AKT-2 dimerize to phosphorylate and inhibit DAF-16, preventing dauer entry [42]. AKT-1 and AKT-2 function has been found to be uncoupled in germline apoptosis following DNA damage [42]. AKT-1 was found to negatively regulate CEP-1 transcriptional activity and the activation of apoptosis [29] independent of DAF-16, while DAF-2 was found to function downstream or independently of AKT-1, regulating apoptosis through AKT-2 and DAF-16 [42]. Similarly, we observed separated AKT isoform function in brap-2 mutants, where a loss of akt-1 restored dauer and increased germline apoptosis more significantly in brap-2 mutants than a loss of akt-2, in a daf-16 independent manner. Like DAF-2, this indicates that BRAP-2 function may be independent of the components upstream of AKT-1.
Although we were unable to test phosphorylated AKT-1 levels to verify whether this increase in AKT-1 corresponded to increased AKT-1 activation, we found that a loss of brap-2 enhanced IIS stimulation and DAF-16 attenuation. We also confirmed that BRAP-2 interacts with PHLP-2 in vitro, placing BRAP-2 as a potential regulator of the IIS pathway. Therefore, we postulate that AKT-1 and SKN-1 activity are modulated by BRAP-2 for regulation of DNA damage induced germline apoptosis. We also identified a potential novel role for PHLP-2 in germline apoptosis where phlp-2 mutants displayed a significant and general reduction in germline apoptosis that was not restored with a loss of PMK-1 (Fig. S10C). Like its mammalian ortholog, BRAP-2 possesses the functional domains of an E3 ubiquitin ligase [2]. We did not verify whether this enzymatic activity is conserved however, it cannot be ruled out as a possible mechanism to post translationally modify and regulate proteins such as PHLP-2, and therefore affect the downstream signaling of these pathways. Similar to brap-2, further investigation is required to determine whether phlp-2 directly influences the core apoptotic pathway or functions through an alternate mechanism to regulate germ cell death.
MPK-1 is required for germline progression and activation of CEP-1 [43], while PMK-1 has been linked to germline apoptosis induced by IR, arsenite and bacterial infection [44]. We have found that BRAP-2 may regulate CEP-1 and the core apoptotic pathway through MPK-1, while influencing apoptosis through PMK-1. Our findings suggest that BRAP-2 regulates DNA damage induced germline apoptosis by blocking the activation of pro-cell survival pathways to promote apoptosis by inhibiting SKN-1 and AKT-1 through PMK-1 and PHLP-2 respectively (Fig. 7). Although further investigation is required, we reveal a potential novel role for PHLP-2, where it may be necessary for the general induction of germline apoptosis.
In addition to mutations in BRCA1, RAS and PI3K/AKT pathway dysfunction has been detected in breast cancer, leading to metastasis and reduced apoptosis [42]. In mammalian systems, it is predicted that BRAP2 prevents BRCA1 from traveling to the nucleus and performing functions that protect genomic integrity [10]. Although, to our knowledge, an apoptotic role for BRAP2 has not be identified, we predict that in combination with mutations in BRCA1, BRAP2 inactivation could promote tumorigenesis, by avoiding death and enhancing cell survival.
Apoptosis is vital for shaping organs and limbs, eliminating self-reactive cells and for protection against malignant transformation. The evasion of apoptosis is a hallmark of cancer and this study has provided us with a genetic tool to better understand the complex and intricate regulation of signaling pathways that govern the balance between pro-death and pro-cell survival factors that lead to apoptosis activation, while discovering potential new targets that regulate apoptosis for future study and therapeutic development.
Materials and methods
C. elegans strains and genetics
All worm strains were cultured under standard conditions, as previously described [43]. Strains were obtained from the Caenorhabditis Genetics Center at the University of Minnesota and from the National Bioresource Project in Tokyo, Japan. Double mutant strains were generated according to standard protocols. The N2 Bristol strain was used as the wild type, and unless noted otherwise all experiments were conducted at 20 °C. C. elegans strains used in this study are listed in Supplemental Material (Table S1).
Fluorescence microscopy
Worms were picked and anesthetized using 2 mM Levamisole (Sigma-Aldrich, Oakville, ON, Canada) and mounted on 2% agarose pad. Images of fluorescent worms were taken using a Zeiss LSM 700 confocal laser-scanning microscope with Zen 2010 Software.
Worm synchronization
For all worm assays worms were synchronized via Egg Lay, as described by the Morimoto Lab (http://groups.molbiosci.northwestern.edu/morimoto/research/Protocols).
Irradiation
Worms were synchronized to the L4 stage and exposed to ionizing radiation using a 137Cs source (in coordination with the Derry Lab, McMaster Building & Sick Kids Peter Gilgan Research Tower) at indicated doses. Worms were allowed to recover for 18–24 h on NGM plates before apoptosis, RNA extraction and qRT-PCR, cytological, and cell cycle arrest analyses.
Germline apoptosis assay
Worms were allowed to recover for 18–24 h and corpses (apoptotic cells) were scored using Acridine Orange (Sigma-Aldrich) staining, as previously described [44,45]. After hatching, mpk-1(ga111) worms were grown at 26 °C (restrictive temperature) [46,47,48]. Worms were anesthetized using 2 mM Levamisole in M9 buffer and mounted on 2% agarose pads where 15–20 gonad arms per genotype were scored per treatment. Apoptotic cells in developing embryos were scored in specific embryonic stages directly using the ced-1p::GFP strain.
DNA staining
To quantify germline nuclei, whole worms were stained with DAPI, as previously described [13]. Z-stack images were taken and using Zen 2010 Lite software the free-form surface area measurement tool was used to measure gonad surface area of one representative slice. Germlines were dissected and stained with DAPI (Vector Labs, Burlingame, CA, USA). ImageJ (National Institute of Health) Cell Counter plugin was used to determine the total number of nuclei per zone in one gonad arm per worm with 40–50 worms per genotype imaged and counted.
Generation of BRAP::GFP transgenic strain
The transgenic strain BRAP-2::GFP was generated, as described previously [49]. The 5′HR arm was amplified using a fosmid template and sub-cloned into NaeI/SalI site of pDD282 (Addgene) using the HiFi Assembly Kit (NEB, Whitby, ON, Canada). 3′HR arm was amplified using the fosmid template and was then sub-cloned into pDD282 in SpeI and AvrII site to create the final plasmid. The single guide RNA (sgRNA) was used to mutate the pDD162 (Addgene) vector.
Whole worm staining
Antibody staining was performed as previously described by the Koelle Lab protocol [50]. Synchronized L4 worms were collected, stained with the designated antibodies and were mounted on 3 µL Prolong Gold Anti-fade reagent (Fisher, Waltham, MA USA). Worms were then visualized with Zeiss LSM 700 confocal microscope at 20x. Fluorescence intensity was quantified from 20 worms for each genotype using ImageJ software. Antibodies used were: anti-ERK1/2(1:500) (Santa Cruz, Dallas, TX, USA), anti-pERK1/2 (1:500) (Sigma-Aldrich), and DAPI (1 µg/mL) (Sigma-Aldrich). Alexa Fluor 488 (1:1000) (Invitrogen) secondary antibody was used for visualization.
RNA interference analysis
Worms were synchronized on RNA interference (RNAi) plates (NGM containing 0.4 mM IPTG, 100 µg/mL Ampicillin and 12.5 µg/mL Tetracycline) and fed with E. coli HT115 (DE3), transcribing double stranded RNA (dsRNA) from fusion bacterial plasmids homologous to the target with pL4440 used as the control. When animals reached the 1st day of adulthood they were then treated and assayed for germline corpse quantification. The skn-1 RNAi plasmid was obtained from a transcription factor specific RNAi library, as described [51]. The akt-1 RNAi plasmid was purchased from GE Dharmacon (Lafayette, CO, USA). The brap-2 RNAi construct was made by amplifying region 1–843 nucleotides of the brap-2 ORF by PCR, using the EST yk1134a01 as template, and cloning into the KpnI site of pL4440. The phlp-2 RNAi construct was made by amplifying the 2618-2800 nucleotide region of the predicted phlp-2 ORF (F43C1.1; Wormbase Version WS258) by PCR, using the EST yk1149d03 as template, and cloning into the KpnI site of pL4440.
RNA isolation and quantitative real time PCR
Worm RNA Isolation, quantitative real time PCR (qRT-PCR) and data analysis was performed, as described previously [21]. mRNA expression levels for each strain was compared relative to the wild type (N2) control, where the housekeeping gene act-1 was used as the endogenous control for normalization. Data represents three biological replicates. To verify equal primer amplification efficiency, a standard curve for each set of primers was constructed using a serial dilution of cDNA. All primers used in this study are listed in Table S3.
DAF-16 localization assay
DAF-16 expression was viewed in vivo using cell type specific reporter strain CF1139. To investigate DAF-16 expression in a brap-2(ok1492) mutant background, transgenic strains were generated using standard C. elegans crossing technique and confirmed by Single Worm PCR. Worms were synchronized to L4 stage and were treated with 5 mM sodium arsenite (Sigma-Aldrich) in M9 Buffer for 1.5 h rotating to induce acute stress [52]. Worms were then paralyzed in 2 mM Levamisole in M9 buffer and mounted on 2% agarose pads. For each genotype, worms were visualized and DAF-16::GFP expression was scored as cytoplasmic, intermediate or nuclear [53].
Dauer defective assay
The dauer defective assay was performed, as previously described [26]. Singleadult worms from each strain were picked onto individual OP50 seeded NGM plates and allowed to grow for 2 weeks to enter dauer developmental arrest. Each plate was flooded with 1 mL of 1% SDS solution and allowed to sit for 30 min. Each plate was then observed under a stereomicroscope to assess the state of the worms. If worms were found to be thrashing on the plate they were declared to have survived (proper entry into dauer). If worms were found to be motionless they were deemed dead (failed to enter dauer). For each genotype, a minimum of 40 starved plates were examined.
Plasmid construction
To construct 3xFLAG::PHLP-2, the C-terminal region of PHLP-2 (1428 bp) was amplified from worm cDNA and the product was cloned in frame to a BamHI and EcoRI digested pCMV 7.1 (3xFLAG) vector. The N-terminal region of PHLP-2 (1682 bp) was amplified from synthesized DNA (BioMatik, Cambridge, ON, Canada) based on Wormbase sequence for F43C1.1 into a NotI and BglII digested pCMV 7.1 (3xFLAG) vector containing the PHLP-2 C-terminal region using the HI-FI Assembly Mix (NEB) following the manufacturer’s protocol.
GST pull down and western blot analysis
Human Embryonic Kidney (HEK) 293 T cells were cultured in Dulbecco’s Modified Eagle’s Medium supplemented with 10% Fetal Bovine Serum. To test for interaction between GST::BRAP-2 and 3xFLAG::PHLP-2, 5 µg DNA of each construct coding these proteins were co-transfected for 48 h using polyethylenimine (Sigma-Aldrich) and Optimem (Fisher). Cells were lysed in lysis buffer (1X TBS, 1% NP-40, 1% Glycerol, 150 mM NaCl, and complete protease inhibitor cocktail (Sigma-Aldrich) and Glutathione Sepharose Beads (GE Healthcare Life Sciences, Mississauga, ON, Canada) were added according to the manufacturer’s protocol with one modification: washing of beads was carried out 6x for 15 min. Cell and worm lysate samples were analyzed by standard SDS polyacrylamide gel, followed by Western Blot, as previously described [21]. The following antibodies were used: anti-FLAG M2 (Sigma-Aldrich), anti-ERK1/2 (Santa Cruz), anti-pERK1/2 antibody (Sigma-Aldrich) anti-GST (NEB), anti-tubulin (NEB), anti-AKT-1 528, and anti-AKT-1 127. Proteins were detected using SuperSignal West Femto Maximum Sensitivity Substrate (ThermoScientific) and blots were imaged using the DNR Bio Imaging Systems Microchemi System. Band density was measured and normalized to their respective anti-tubulin protein levels using the gel analyzer plugin in ImageJ software.
Statistical analysis
Statistical analysis was performed with GraphPad Prism® 7 Software. Statistical significance was determined using Two-way ANOVA and Bonferroni post tests for comparison between different groups. Student’s t-test was performed when comparing two means. A chi-square test was used for analysis of DAF-16::GFP localization [54]. P values of < 0.05 were taken to indicate statistical significance (***p < 0.001, **p < 0.01, *p < 0.05). Error bars represent ± standard error of the mean.
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Acknowledgements
A number of strains were provided by the CGC, which is funded by NIH Office of Research Infrastructure Programs (P40 OD010440) and the National BioResource Project. AKT antibodies were a kind gift from W.B. Derry of Hospital for Sick Children, Toronto. We thank the Derry Lab (E. Chapman, B. Yu, B. Lant, A. Mateo, M. Hall, M. Gunda and M. Haeri) for their assistance with the irradiation. D.R.D. is a recipient of the Ontario Graduate Scholarship. M.P. is a recipient of the Dean’s Award from the Faculty of Science, York University and a Natural Sciences and Engineering Research Council of Canada (NSERC) undergraduate studentship. D.R.D., Q.H., M.P. and T.J.K were supported by a grant from NSERC.
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D’Amora, D.R., Hu, Q., Pizzardi, M. et al. BRAP-2 promotes DNA damage induced germline apoptosis in C. elegans through the regulation of SKN-1 and AKT-1. Cell Death Differ 25, 1276–1288 (2018). https://doi.org/10.1038/s41418-017-0038-7
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DOI: https://doi.org/10.1038/s41418-017-0038-7