Abstract
Accumulating research implicated that circular RNAs exhibited significant roles in cancer development. Nonetheless, the role regarding circPTPN22 in pancreatic cancer remains unclear. Expression of circPTPN22 in pancreatic cancer cell lines and normal cells was determined with quantitative real-time PCR (qRT-PCR). Cell counting kit-8 assay and colony formation assay were used to measure the proliferation of pancreatic cancer cells. RNA immunoprecipitation and Western blot were employed for investigation the binding between circPTPN22 and STAT3. circPTPN22 expression was highly upregulated in pancreatic cancer tissues and cell lines. Knockdown of circPTPN22 inhibited cell proliferation and attenuates pancreatic cancer immune microenvironment. Furthermore, STAT3 acetylation was involved in these effects. circPTPN22 promoted STAT3 acetylation via inhibiting STAT3/SIRT1 interaction. circPTPN22 attenuates pancreatic cancer immune microenvironment by promoting STAT3 acetylation via inhibiting STAT3/SIRT1 interaction.
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Funding
Shanxi Province “1331 project” Key Innovation Team Fund; Major scientific research project of Wuxi Municipal Health Commission (No. Z201805); Scientific and Technological Innovation Programs of Higher Education Institutions in Shanxi (2019L0685); Dr. Changzhi Medical College Startup Fund (BS201903).
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Conception and design: YH, PH, QZ and CL. Data analysis and interpretation: CC, SX, HZ, YS and HH. Drafting of the manuscript: HY. Critical revision of the manuscript for important intellectual content: QZ and CL. Final approval of the manuscript and submission: YH, PH, QZ, and CL. All authors read and approved the final manuscript.
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This study was reviewed and approved by the Ethics Review Board of Heping Hospital. Written informed consents were obtained from each participant. The experiment was conducted in line with the Declaration of Helsinki.
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He, Y., Han, P., Chen, C. et al. circPTPN22 attenuates immune microenvironment of pancreatic cancer via STAT3 acetylation. Cancer Gene Ther 30, 559–566 (2023). https://doi.org/10.1038/s41417-021-00382-w
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DOI: https://doi.org/10.1038/s41417-021-00382-w