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LncRNA KCNQ1OT1 facilitates the progression of bladder cancer by targeting MiR-218-5p/HS3ST3B1


Long non-coding RNA (lncRNA) is characterized by biological function in diverse cancers. LncRNA KCNQ1 opposite strand/antisense transcript 1 (KCNQ1OT1) is well acknowledged to regulate various cancers, while its role in bladder cancer remains unclear. In the present study, we aimed at probing into the impact and detailed mechanisms of KCNQ1OT1 in bladder cancer progression. In this study, we demonstrated that KCNQ1OT1 expression in bladder cancer tissues was notably up-regulated compared with in normal adjacent tissues, and KCNQ1OT1 modulated the malignant phenotypes of bladder cancer cells. Moreover, it was validated that KCNQ1OT1 could specifically bind to miR-218-5p and reduce its expression. Overexpressed miR-218-5p would inhibit the proliferation and metastasis of bladder cancer cells while facilitating apoptosis. In terms of Mechanism, Heparan Sulfate-Glucosamine 3-Sulfotransferase 3B1 (HS3ST3B1) was validated as a target gene of miR-218-5p, and could be regulated by KCNQ1OT1 indirectly. In conclusion, KCNQ1OT1 can promote the progression of bladder cancer through regulation of miR-218-5p/HS3ST3B1, which is expected to serve as a new therapeutic target for bladder cancer.

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Fig. 1: The expressions of KCNQ1OT1 in bladder cancer tissues and cell lines were detected by qRT-PCR.
Fig. 2: The impact of overexpression and knockdown of KCNQ1OT1 on proliferation, migration, invasion and apoptosis of SW780 and T24 cell lines, respectively.
Fig. 3: The expressions of KCNQ1OT1 and miR-218-5p and their interaction in bladder cancer tissues and cells.
Fig. 4: The impact of overexpression and inhibition of miR-218-5p on proliferation, migration, invasion, and apoptosis of T24 and SW780 cell lines, respectively.
Fig. 5: The effects of KCNQ1OT1/miR-218-5p on proliferation, migration, invasion, and apoptosis of bladder cancer cells.
Fig. 6: The expressions of miR-218-5p and HS3ST3B1 and their interaction in bladder cancer tissues and cells.

Data availability

The data used to support the findings of this study are available from the corresponding author upon request.


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Correspondence to Yili Liu.

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Our study was approved by the ethics review board of the Fourth Affiliated Hospital of China Medical University.

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Li, Y., Shi, B., Dong, F. et al. LncRNA KCNQ1OT1 facilitates the progression of bladder cancer by targeting MiR-218-5p/HS3ST3B1. Cancer Gene Ther 28, 212–220 (2021).

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