Thymidylate synthase drives the phenotypes of epithelial-to-mesenchymal transition in non-small cell lung cancer

Background Epithelial-to-mesenchymal transition (EMT) enhances motility, stemness, chemoresistance and metastasis. Little is known about how various pathways coordinate to elicit EMT’s different functional aspects in non-small cell lung cancer (NSCLC). Thymidylate synthase (TS) has been previously correlated with EMT transcription factor ZEB1 in NSCLC and imparts resistance against anti-folate chemotherapy. In this study, we establish a functional correlation between TS, EMT, chemotherapy and metastasis and propose a network for TS mediated EMT. Methods Published datasets were analysed to evaluate the significance of TS in NSCLC fitness and prognosis. Promoter reporter assay was used to sort NSCLC cell lines in TSHIGH and TSLOW. Metastasis was assayed in a syngeneic mouse model. Results TS levels were prognostic and predicted chemotherapy response. Cell lines with higher TS promoter activity were more mesenchymal-like. RNA-seq identified EMT as one of the most differentially regulated pathways in connection to TS expression. EMT transcription factors HOXC6 and HMGA2 were identified as upstream regulator of TS, and AXL, SPARC and FOSL1 as downstream effectors. TS knock-down reduced the metastatic colonisation in vivo. Conclusion These results establish TS as a theranostic NSCLC marker integrating survival, chemo-resistance and EMT, and identifies a regulatory network that could be targeted in EMT-driven NSCLC.

between passages 3 and 15, examined for mycoplasma and maintained in Plasmocin (Invivogen) to prevent contamination.

Lentiviral transduction
Plasmids for TS knock down (TRCN0000456666 for human cell lines and TRCN0000317583 for murine cell lines) are from Sigma. Scrambled pLKO.1 (referred to as pLKO) was used as control. Plasmids from TYMS-promoter reporter (HPRM33357-LvPM02), GAPDH promoter reporter (HPRM39787-LvPM02), TS expression vector (Ex-T0406-LV105b) and control vector (Ex-Neg-LV105b) are from GeneCopoeia. For production of lentiviral particles, 293T cells were transfected with 8 µg knock-down/expression vectors and 2 µg of pMDL, pVsVg and pRevRes in complex with 24 µg PEI (Polysciences). After 48 h, supernatant was collected, centrifuged and filtered. For transduction, 10 5 cells were seeded in a six-well plate and infected in presence of 8 μg/ml polybrene (Sigma). Selection was done with 3 μg/ml puromycin (Sigma) and cells were maintained in 1 µg/ml puromycin. RNA sequencing Total RNA was extracted using miRNeasy kit (Qiagen) following the manufacturer's instructions. RNA-Seq libraries were constructed using the TruSeq sample Prep Kit V2 (Illumina). Briefly, 1 μg of purified RNA was poly-A selected and fragmented with fragmentation enzyme. After first and second strand synthesis from a template of poly-A selected/fragmented RNA, other procedures from endrepair to PCR amplification were done according to library construction steps. Libraries were purified and validated for appropriate size on a 2100 Bioanalyzer High Sensitivity DNA chip (Agilent Technologies). The DNA library was quantified using Qubit and normalised to 4 nM before pooling. Libraries were pooled in an equimolar fashion and diluted to 10 pM. Library pools were clustered and run on Nextseq500 platform with paired-end reads of 75 bases, according to the manufacturer's recommended protocol (Illumina). Raw reads passing the Illumina RTA quality filter were preprocessed using FASTQC for sequencing base quality control. Sequence reads were mapped to UCSC human genome build using TopHat and differential gene expression determined using Cufflinks 2.1.1 and Cuffdiff2.1.1 as implemented in BaseSpace. The sequencing data has been submitted GEO dataset and could be accessed with GSE148589 accession number.
Quantitative real-time PCR Total RNA was extracted using miRNeasy kit (Qiagen) and 50 ng was converted to cDNA using Tetro cDNA synthesis kit (Bioline) with random hexamers. GAPDH was used as an internal control. TaqMan probes (Thermo-Fisher) were used for quantification in Applied Biosystems 7300. Fold change was calculated using the ΔΔCt method.
Gene set enrichment analysis Gene set enrichment analysis (GSEA), for computing overlap, on the differentially expressed genes upon TS knockdown was performed with the gene set collections in the Molecular Signatures Database v6.1 software. For EMT gene set enrichment analysis in the patient data, normalised gene expression values were downloaded from GEO database (GSE101929) and cbioportal platform for TCGA profile (LUAD, PanCaner). For calculation of TS Knockdown (KD) score, first, z scores of the down-and up-regulated genes upon TS knockdown were calculated. Then, the sum of z scores of downregulated genes was subtracted from the sum of z scores of upregulated genes and KD scores were obtained for each patient. Patients were grouped for the analysis based on either the median value of TYMS gene expression or KD score.

Survival analysis
Normalised gene expression profiles of lung cancer samples were downloaded from GEO (GSE50081, GSE72094, GSE30219) and mRNA expression values as Z-scores were obtained for TCGA profiles (LUAD and LUSC) from cbioportal platform. Thirty-five samples from completely resected NSCLC patients were collected from the files of San Luigi Hospital, Orbassano, Turin, Italy. None of the patients received either neo-adjuvant chemotherapy or radiation therapy and all received adjuvant cisplatin and pemetrexed. All cases were reviewed and classified using anonymised samples. Clinical samples were stratified as TYMSlow and TYMS-high based on the median value of gene expression as cut-off. Kaplan-Meier estimate was used to generate survival curves and significance between the two groups were analysed using log-rank test in R software. Survival graphs from the KM Plotter database was generated based on TYMS expression by using the auto select best cut-off option. TS KD score for survival curve was calculated as described in the previous section.
Proliferation assay For proliferation assay cells were seeded in 96-well plates in low density (5-20% initial confluency). Plates were loaded in IncuCyte-Zoom (Essen Bioscience) and scanned every 2-4 h. For each scan, phase contrast image was acquired from every well and was analysed by IncuCyte Zoom software.
In vitro drug treatment Pemetrexed was purchased from Sigma. For in vitro treatment cells were plated in a 96-well plate (4000 cells/well) and incubated overnight. For cytotoxicity death assay, 2000X Cytotox Green Reagent (Essen Bioscience) was diluted in RPMI and working dilutions of pemetrexed was prepared in Cytotox Green supplemented media. After treatment, plate was loaded in Incycuyte Zoom and images were acquired in real-time for phase to quantify growth. Activity of Cytotox reagent was simultaneously acquired at the green channel to quantify death. Incycuyte Zoom software was used for the analysis and data export.
Migration assay For migration assay cells were plated in 96-well plates so that they reach 90% confluency overnight. Cells were wounded using WoundMaker (Essen Biosciences) as per the instruction from the manufacturer. Plates were loaded in IncuCyte Zoom and were automatically scanned for programmed time interval. For each scan, wound width was recorded by the software and the proliferation inside the wound was normalised to the proliferation outside the wound, giving relative wound density for each time point.
Tumoursphere culture In all, 40,000 cells were seeded in triplicates in ultra-low attachment six-well plates (Corning) in complete Mammocult medium (Stem Cell Technologies), prepared according to the manufacturer's instruction. After formation, spheres were counted by spinning at 300 g for 5 min and suspending in PBS (Lonza). siRNA transfection Reverse transfection was done with Lipofectamine RNAiMAX Transfection Reagent (Thermo). 50 nM siRNA were mixed with 1.5 µl transfection reagent in 200 µl Opti-MEM (Thermo) and incubated for 15 min. After incubation transfection complex was added to the surface of a 12-well plates and 10 5 cells, suspended in 800 µl, were added. Cells were incubated at 37°C, 5%CO 2 . Cells were lysed for western blot after 72 h.
In vivo experiments C57BL/6 strain were used as experimental model to study effect of Ts-depletion in syngeneic LL/2 cells to prevent immune rejection. 13 Mice were anaesthetised using isoflurane and euthanised by cervical dislocation.
For tail-vein metastasis assay, 5 × 10 5 LL/2 pLKO and shTs cells were resuspended in 100 µl PBS and injected in the tail vein of female C57BL/6, with 10 mice per group. Lung metastases were monitored by bioluminescence imaging (BLI) 4 weeks after injection. Anesthetised mice were intraperitoneally injected with 150 mg/kg D-luciferin (Kayman Chemicals). Bioluminescence images were acquired with Lumina III in vivo Imaging System (IVIS, Perkin Elmer). For all the mice exposure time was maintained at 180 s. Raw IVIS images were analysed with Living Image software and the metastasis was represented as radiance.

Statistical analysis
Statistical tests were performed with the GraphPad software v.7 comparing groups of different conditions with replicates. In all tests, the statistical significance was set at p ≤ 0.05.  Each dot represents a single gene. c, d Predicted overall survival in non-small cell lung cancer (NSCLC) patients separated according to the expression level of TS. p-value is Logrank test. Sensitivity of NSCLC cells lines A549 (e) and Calu-1 (f) to anti-folate drug pemetrexed (Pem) after shRNA-mediated knock-down of TS. Dead cells have been quantified as green object counts. p-value is represented as two-way ANOVA, Sidak's multiple test. g Kaplan-Meier survival analysis of NSCLC patients treated with pemetrexed-based chemotherapy categorised according to high and low TS mRNA expression. p-value is Logrank test. h TYMS mRNA levels compared between NSCLC epithelial (Epi) and mesenchymal (Mes) cells from Broad Institute Cancer Cell Line Encyclopedia (CCLE) data, defined by VIM/CDH1 mRNA expression ratio. p-value is from a Student's t-Test. i, j Gene-set enrichment analysis of high and low TS correlating with published EMT gene signature (indicated on the top of the graphs) in lung adenocarcinoma subset of the Cancer Genome Atlas (TCGA) dataset. Error bars represent standard deviation.

RESULTS
TS is an essential NSCLC gene with prognostic/predictive power and correlates with EMT signatures We evaluated different clinical aspects of TS in NSCLC and assayed its correlation with EMT. As a rate-limiting de novo pyrimidine biosynthesis enzyme, TYMS (gene coding TS) has been proposed as an essential gene, but so far, no functional data have been shown in NSCLC. To evaluate dependency of NSCLC on TS, a dataset generated from a genome-wide CRISPR/Cas9 screen of 18,009 genes in 324 cancer cell lines was exploited. 14 Based on a gene fitness score that defined how strongly a cancer is dependent on a gene for survival and growth, a priority score was generated to identify the most promising drug targets. Among all the pan-cancer fitness genes identified, TS ranked 30th (top 1%, Fig. 1a, see Supplementary Table 1 for the top 50 genes). In NSCLC subsets, it ranked 2nd and 19th in squamous cell carcinoma (SCC) and adenocarcinoma (ADC), respectively (Fig. 1b), indicating that NSCLC strongly depend on TS for sustained growth. TS was also found to be 5th among the pan-cancer priority targets identified ( Supplementary Fig. 1A). TS was the only target of pemetrexed that exclusively appeared as significant in all the three lists ( Supplementary Fig. 1A-C). In concordance, TS expression has been consistently found increased in NSCLC compared to adjacent normal tissues 15 and correlated with poor prognosis in different expression datasets analysed (Fig. 1c, d). TS is targeted by the anti-folate drug pemetrexed, and its overexpression has been proposed to determine chemoresistance. 16 For in vitro validation, we established shRNA-mediated TS knockdown in two NSCLC cell lines and observed a significant increase in pemetrexed sensitivity (Fig. 1e, f). To test if in vitro evidence were also reflected in the outcome of chemotherapytreated patients, we retrospectively analysed a small case-series of NSCLC patients treated with pemetrexed-based chemotherapy and found that higher TS gene expression significantly associated with worse prognosis (Fig. 1g). These results emphasise the importance of TS as a prognostic and predictive marker, in line with previous literature. 10 However, chemoresistance is also an important hallmark of EMT, and recent pivotal findings from our lab associated TS expression with EMT markers in cancers from different origins and suggested a potential direct role. 11 To test this in NSCLC, we analysed cells belonging to the CCLE dataset and found that lung cancer cell lines with high TS expression have enrichment in EMT signature genes (Supplementary Fig. 1F, G). When further categorised as epithelial or mesenchymal based on ratio of Vimentin (VIM) and E-Cadherin (CDH1) expression, 17 ( Supplementary Fig. 1H) mesenchymal-like cells expressed higher TS compared to epithelial-like (Fig. 1h). To further demonstrate its clinical significance, we investigated multiple datasets and found that patients with higher TS expression were significantly enriched for hallmark EMT genes (Fig. 1i, j and Supplementary Fig. 1I, J).
These results indicate that TS is not only an essential proliferation gene with a strong prognostic and predictive role, but also has a potential power in EMT in NSCLC.
Endogenous TS level is an important determinant of EMT phenotype TS expression has been shown to be highly varied in clinical samples stained for immunohistochemistry 15,18 and bioinformatic analysis revealed that, within a tumour, individual cells can have extremely diversified TS expression (Supplementary Fig. 2A, B). When mapped to a published gene signature, individual cells with higher TS expression within a tumour showed a significant enrichment of EMT ( Supplementary Fig. 2C). Hence, we postulated that intrinsic level of TS could be a strong determinant of EMT. To functionally validate the hypothesis, Calu-1 (a SCC cell line) was stably transduced with a promoter reporter construct 19 that expressed mCherry fluorescent protein transcribed from TYMS promoter (Fig. 2a). After puromycin selection, cells were FACSsorted for highest and lowest red fluorescence (indicated further as TS HIGH and TS LOW , Fig. 2b). TS LOW cells proliferated slower (Fig. 2c) and showed a distinct epithelial phenotype, whereas TS HIGH resembled a mesenchymal-like morphology (Fig. 2d). To confirm differential EMT status at molecular level, expression of E-CAD and VIM, markers for epithelial-like and mesenchymal-like cells respectively, was quantified. At mRNA level, even with a minimal difference in TS expression, there was a striking difference between the expression of CDH1 (gene coding E-CAD) and VIM (Fig. 2e). TS LOW cells also expressed more E-CAD and lesser VIM compared to TS HIGH cells at protein level (Fig. 2f), backed up by E-CAD changes observed in Calu-1 cells with knockdown and overexpression of TS ( Supplementary Fig. 2D). Also, with the knockdown of TS, there was proliferation loss in the cells ( Supplementary Fig. 2E) as observed with the cells after sorting. When assayed in proliferation-normalised wound migration assay, TS LOW cells migrated slower than TS HIGH cells (Fig. 2g, h). As a control for the promoter reporter assay, Calu-1 cells were sorted for GAPDH promoter activity and no difference in EMT markers and migration was observed in GAPDH HIGH and GAPDH LOW cells (Fig. 2f-h). TS LOW cells also had reduced self-renewal capacity, quantified as the number of tumourspheres formed in a lowadherence culture (Fig. 2i). Further validation came from A549, that we had previously characterised to have lost stem cell phenotype after TS knockdown. 11 When sorted in TS HIGH and TS LOW cells ( Supplementary Fig. 2F), A549 cells, that exist in partial EMT state, 20 recapitulated the EMT phenotypes observed in Calu-1 (Fig. 2j, k), although change in the self-renewal capacity was not observed (Supplementary Fig. 2G). We further independently validated TS-mediated EMT by knocking down TS in NSCLC cell lines SK-MES-1 (SCC cell line) and NCI-H23 (ADC cell line), where TS depletion led to upregulation of E-CAD and downregulation of VIM in SK-MES-1 (Supplementary Fig. 2H) and downregulation of VIM and ZEB1 (mesenchymal marker) in NCI-H23 ( Supplementary  Fig. 2I). Interestingly, a rapid reversion of EMT phenotype was observed in the sorted Calu-1 cells, concomitant with the normalisation of TYMS promoter activity ( Supplementary Fig. 2J). This was more evident in functionally distinct A549 cells, where the sorted cells showed higher TS levels in TS HIGH a day after sorting, followed by a complete normalisation of TS and EMT markers after few passages ( Supplementary Fig. 2K). Therefore, the phenotypic alterations observed between sorted cells were transient and in match with the differences in TS levels. These data strongly indicate a direct control of TS on EMT phenotype and hints that TS might have role to play in epithelial plasticity.
TS regulates EMT genes in NSCLC Further, to identify the mediators of TS-promoted EMT, RNA was sequenced from Calu-1 TS HIGH and TS LOW cells in parallel with A549 cells with TS knockdown. Where Calu-1 sorted cells have shown growth reduction (Fig. 2c), A549 cells with TS knock-down proliferated normally (Supplementary Fig. 3A). Hence, two different cell lines that either have or don't have a loss in proliferation, and that are processed by two independent techniques, were subjected to investigation. Pathway analyses consistently indicated EMT among the topmost differentially regulated pathways (Fig. 3a, b), confirming the EMT switch observed with E-CAD and VIM (Fig. 2f, j). KRT19, SPARC, SPOCK, LINC00707 (lung cancer promoting lincRNA), FOSL1 and AXL (identified as downstream targets of TS as they appeared in both signatures) were qPCR validated in both cell lines ( Fig. 3c and Supplementary Fig. 3B). Of these genes, SPARC, FOSL1 and AXL, that have an established role in EMT in NSCLC, [21][22][23] were strongly down-regulated at protein level in A549 cells with TS knockdown (Fig. 3d). Differentially expressed genes (DEGs) were used to derive a knockdown score, which predicted a worse survival associated with lower TS knockdown (higher TS levels, Fig. 3e) and correlated with published EMT gene signature (Fig. 3f). This indicated that TSmediated EMT is empowered with its own prognostic impact, i.e. contributes to the adverse prognosis of NSCLC with high TS levels (Fig. 1c, d), suggesting a role for TS beyond proliferation. Several EMT transcription factors (HMGA2, HOXC6, SNAI2, SOX9, ARNTL2, SHOX6) were identified from DEGs in endogenously TS HIGH and TS LOW cells, from which siRNA mediated knock-down of HOXC6 and HMGA2 reduced expression of TS in Calu-1 ( Supplementary  Fig. 3C) and was validated in A549 (Fig. 3g). Thus, these results identified a network of TS mediated EMT, where HOXC6 and HMGA2 are upstream of TS and AXL, SPARC and FOSL1 are downstream mediators.

Depletion of TS mitigates metastasis in vivo
Finally, in vivo approaches were used to confirm the role of TS on EMT and metastasis. Ts (mouse TS) expression was quantified in morphologically and functionally distinct mesenchymal-like (Ladi 3.1) and epithelial-like (Ladi 2.1) cells ( Supplementary Fig. 4A, B), isolated from the same mouse model of NSCLC (p53 fl/fl-LSL KRAS G12D/+ ). Ts positively correlated with Vim and negatively with E-Cad ( Supplementary Fig. 4C). Furthermore, to functionally evaluate in vivo effects of TS alteration on metastatic colonisation, Tyms gene was knocked down in murine Lewis lung carcinoma cell line LL/2 using stably transduced shRNA. A moderate Ts depletion (Fig. 4a) did not affect proliferation ( Supplementary  Fig. 4D), as we had previously determined from breast cancer cell lines, that TS needs to reduce beyond a threshold to diminish proliferation. 12 Furthermore, Ts knockdown also did not hamper the growth of primary tumours from the cells subcutaneously injected in flanks of syngeneic mice (Fig. 4b). However, when injected in the tail vein, knocked down cells showed a highly significant reduction in lung metastatic colonisation (Fig. 4c, d), and the mice carrying cells with Ts depletion showed a significantly prolonged survival (Fig. 4e).

DISCUSSION
TS has been widely used as a chemotherapeutic target 24 ascribed to its role in proliferation. This study experimentally validated this concept, as TS was one of the highest-ranked target gene identified based on the CRISPR/Cas9 screen analysed in this study. However, chemotherapeutic drugs that target TS might also mitigate other detrimental features associated with cancer. We confirmed the multifaceted role of TS in proliferation and chemoresistance and found a strong correlation with EMT gene signatures and prognosis, highlighting clinical, as well as biological relevance of TS in NSCLC. Interestingly, presented results clearly corroborate that different functions of TS can operate independently, as observed that TS depletion can mitigate EMT phenotypes without triggering proliferation loss. This we observed in two independent setups-first in A549, showing no loss in proliferation but alteration in EMT signature pathway after TS knockdown, and second in LL/2 cells that reduced metastatic colonisation after TS knockdown that didn't affect proliferation and growth of primary tumour. It strongly adds to the proliferation-independent loss of differentiation that we had previously propounded, 12,25 providing a strong rationale to revisit clinical and therapeutic aspects of TS in tumour biology and explore its therapeutic potential beyond proliferation.
Pemetrexed is used as a first-line treatment for NSCLC patients 26,27 albeit response to the drug is remarkably varied. TS expression is an important determinant of sensitivity to pemetrexed 16,28 and marks the worse clinical outcomes of pemetrexed treatment in NSCLC patients. 29 In agreement to this, SCC generally respond poorly to pemetrexed as compared to ADC, 30 partly attributed to higher TS expression in SCC compared to other histological subtypes. 15,31 However, there is also a considerable variability to pemetrexed response within a given histological subtype. 32,33 EMT could be an additional source of variability, as it is a key driver of chemoresistance against pemetrexed in NSCLC. 34 In this study we have linked higher TS expression not only to pemetrexed outcome but also to EMT, indicating that TS has an important role in establishing a connection between EMT and pemetrexed resistance. This connection could also be extrapolated to genes that constitute the TS regulatory network, such as FOSL1, which regulates pemetrexed resistance in coordination with EMT-TF ZEB1. 22 Therefore, a more inclusive biomarker signature needs to incorporate EMT genes and downstream genes like FOSL1, in addition to TS, for robust prediction of response to pemetrexed in NSCLC. 35 TS expression has been previously shown to be stimulated by chemotherapy, as a cellular defence mechanism, 36 and these data add the notion that chemotherapy-induced TS could lead to the adjustment of EMT phenotypes in patients, that, in turn, might influence the efficacy of treatment. This aspect could be taken into consideration for the implementation of therapeutic strategies combining EMT-suppressing drugs and chemotherapy, or for the future design of the next generation of TS-inhibitors, which should not enhance TS levels.
In NSCLC, EMT enhances the inflammatory tumour microenvironment leading to activation of multiple immune checkpoint proteins, including PD-L1. 37 A recent clinical trial has demonstrated a better outcome in NSCLC when pemetrexed is administered in combination with a PD-L1 inhibitor, pembrolizumab. 38 Since, TS drives EMT and EMT has been shown to modulate response to immune therapy, a functional correlation between TS expression and susceptibility to immunotherapy could be deduced in NSCLC. In fact, we identified interleukins such IL-6, IL-7 and IL-32 in our TS signatures, which have been previously linked with poor prognosis and metastasis in NSCLC. [39][40][41] Hence, a follow-up study is needed to validate this correlation, as the two drugs are frequently combined.
The present study also underscores the plasticity of cancer cells with mixed EMT population, as was reported in cells sorted for high and low TS expression ( Supplementary Fig. 2J, K). It could be interesting to understand how TS mechanistically drives plasticity by molecular profiling of cells at several time points between the sorting and phenotype reversal.
We furthermore establish the role of TS in metastasis, where TS knock-down abrogated the metastatic colonisation and improves mice survival without affecting proliferation and growth of primary tumour. This observation indicates that TS strongly influences the success against selection pressure at the metastatic site. Further retrospective validation in patients can establish TS as a metastasis marker in NSCLC.
Finally, this study provides a perspective for a network that could integrate different signalling pathways to effectuate various aspects of cancer progression that are mediated by TS (Supplementary Fig. 4E), worth further investigation. Different transcriptional regulators and effector proteins identified in this study have an established role in EMT in NSCLC and connect with master EMT-TFs. HMGA2, for instance, affects proliferation and metastasis by regulating TWIST, 42 FOSL1 regulates chemotherapy, exogenous SPARC promotes invasion and metastasis by activating SNAI1 21,42 and AXL activates TWIST to affect cell cycle. 23 A followup study could further substantiate TS as an integration point for these pathways resulting in a cumulative readout in terms of metastasis.
Thus, this study provides strong evidence that TS, apart from proliferation enzyme, also regulates EMT in NSCLC. Targeting EMTrelated processes could represent a promising therapeutic strategy to suppress the aggressiveness of TS-overexpressing NSCLC.

AUTHOR CONTRIBUTIONS
M.A.S. was involved in conception, design, acquisition, and analysis of in vitro experimental data and drafting of the paper. P.N.G. was involved in acquisition and analysis of in vivo experimental data. V.R. was involved in data acquisition and bioinformatic analysis. B.P., A.S. and M.E.V. were involved in in vitro data acquisition, R.N. performed RNA-sequencing, O.S. (Ozge Saatci) performed analysis of RNA-Seq data, I.R. and P.B. acquired patient data, H.S. helped in design of the in vivo work, I.A. A. helped with interpretation of RNA-seq Data, O.Sa (Ozgur Sahin) helped with generation and interpretation of knock down score and critical revision of the paper, M.V. was involved in interpretation of patient data and critical revision of the paper, P. C. was involved in project supervision, experimental conceptualisation and design, analysis and interpretation of data and drafting of paper. All authors read and approved the final paper.  Fig. 4 TS expression determines metastatic colonisation in vivo. a Western blot quantification of Ts in murine LL/2 cells. Scrambled pLKO has been used as a non-targeting control. b Quantification of primary tumour growth after subcutaneous injection of 1 × 10 6 LL/2 cells from a in C57BL/6 mice (n = 8). p-value is represented as Student's t-Test. c, d Quantification of metastatic localisation of luciferase expressing Ts knock-down and pLKO LL/2 cells in lungs of C57BL/6 mice. 5 × 10 5 cells were injected in the tail-vein of mice (n = 10) and the luciferase activity was quantified by IVIS as readout for lung metastasis after 4 weeks. e Survival curves from mice in d. p-value is represented as Log-rank (Mantel-Cox) test. Error bars represent standard deviation.