Fig. 5 | British Journal of Cancer

Fig. 5

From: Metabolic reprogramming and Notch activity distinguish between non-small cell lung cancer subtypes

Fig. 5

Canonical Notch signalling pathway is upregulated in human lung SCC but not AdC and correlates with the SCC-specific metabolic genes. a A heat map of the expression of Notch downstream targets from the Hes and Hey families and MYC, along with Notch ligands of the Jag family in NC, AdC and SCC tissues from the Hou database.19 Corroborating heat maps from additional databases can be found in Fig. S7a, c. b Notch pathway activation in individual samples from the Hou database19 based on the expression of downstream Notch targets (see Experimental Procedures for full details). c Nuclear activated Notch1 (N1ICD) scoring in NSCLC tissues based on immunohistochemistry reanalysed from Westhoff et al.47 One is low expression and 3 is high. Fisher’s Exact Test (P = 0.037, χ2 = 6.617 and df = 2). d The expression of downstream Notch targets assessed in AdC (n = 51) and SCC (n = 28) cell lines from the Broad-Novartis Cancer Cell Line Encyclopedia. Data are shown as box and whisker plots where whiskers show the min and max. *P < 0.05 and **P < 0.01. Significance was determined by two-tailed t-test. e The expression of the SCC gene signature assessed in cell lines from the Broad-Novartis Cancer Cell Line Encyclopedia with low (n = 39) and high (n = 40) expression of downstream Notch targets. Data are shown as box and whisker plots where whiskers show the min and max. *P < 0.05 and **P < 0.01. Significance was determined by two-tailed t-test. See also Fig. S7, Table S6, S7

Back to article page