Fig. 6 | British Journal of Cancer

Fig. 6

From: Oncofoetal insulin receptor isoform A marks the tumour endothelium; an underestimated pathway during tumour angiogenesis and angiostatic treatment

Fig. 6

Insulin receptor antibodies inhibit angiogenesis in vivo. a CAMs were treated with vaso-occlusive Visudyne®-photodynamic therapy in the areas indicated with circles. Representative fluorescence angiographies of CAMs before (pre-PDT), 24 and 48 h after PDT are shown. Anti-INSR monoclonal antibody or a polyclonal antibody were administered topically immediately after irradiation, where indicated. The antibody treatment was repeated 24 h later. Angiogenesis in the irradiated zone was imaged 48 h after start of treatment. b Images were quantified using ImageJ software, and quantification using the branching points descriptor is shown. Right images show the concentric circles indicating the 4 treatment areas, and the skeletonised plot of the vasculature. Scale bar represents 200 μm. *P < 0.05, **P < 0.01 by ANOVA with Bonferroni’s Multiple Comparison test, N = 3

Back to article page