Fig. 5 | British Journal of Cancer

Fig. 5

From: Oncofoetal insulin receptor isoform A marks the tumour endothelium; an underestimated pathway during tumour angiogenesis and angiostatic treatment

Fig. 5

Insulin receptor antibodies inhibit EC function in vitro. a Inhibition of HUVEC and HMEC migration by anti-INSR polyclonal antibodies (pAb) as determined by scratch wound assay. A minor inhibitory effect is observed with low concentrations of antibody (5 μg/ml), whereas considerable inhibition is observed with high concentrations of antibody (50 μg/ml). **P < 0.01 by ANOVA with Bonferroni’s Multiple Comparison test, N = 2–10. b Inhibition of HUVEC and HMEC cell viability by treatment with anti-INSR pAb. **P < 0.01 by ANOVA with Bonferroni’s Multiple Comparison test, N = 14–18. c Antibody interference in the HUVEC sprouting assay with representative images of sprouting spheroids for Ctrl and treated with pAb at 50 μg/ml. Total sprout length (shown here), as well as mean length of the sprouts and number of sprouts per spheroid (Fig. 5) were reduced. **P < 0.01 by ANOVA with Bonferroni’s Multiple Comparison test, N = 7–12. df Effect of INSR knockdown by siRNA (d) on proliferation (e) and sprouting (f) of HUVEC cells was evaluated and quantified. Efficient knockdown was accomplished at the RNA level, which resulted in only a slight reduction in cell viability, but which had a pronounced impact on endothelial sprouting. Values are presented relative to control siRNA. *P < 0.05, **P < 0.01 by ANOVA with Bonferroni’s Multiple Comparison test, N = 4 (d), N = 9 (e), N = 11 (f)

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