Important factors in implementation of lineage-specific chimerism analysis for routine use

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References

  1. 1.

    Bader P, Niethammer D, Willasch A, Kreyenberg H, Klingebiel T. How and when should we monitor chimerism after allogeneic stem cell transplantation? Bone Marrow Transpl. 2005;35:107–19.

    CAS  Article  Google Scholar 

  2. 2.

    Clark JR, Scott SD, Jack AL, Lee H, Mason J, Carter GI, et al. Monitoring of chimerism following allogeneic haematopoietic stem cell transplantation (HSCT): Technical recommendations for the use of Short Tandem Repeat (STR) based techniques, on behalf of the United Kingdom National External Quality Assessment Service. Br J Haematol. 2015;168:26–37.

    Article  Google Scholar 

  3. 3.

    Thiede C, Florek M, Bornhäuser M, Ritter M, Mohr B, Brendel C, et al. Rapid quantification of mixed chimerism using multiplex amplification of short tandem repeat markers and fluorescence detection. Bone Marrow Transpl. 1999;23:1055–60.

    CAS  Article  Google Scholar 

  4. 4.

    Lawler M, Humphries P, McCann SR. Evaluation of mixed chimerism by in vitro amplification of dinucleotide repeat sequences using the polymerase chain reaction. Blood. 1991;77:2504–14.

    CAS  Article  Google Scholar 

  5. 5.

    Kletzel M, Huang W, Olszewski M, Khan S. Validation of chimerism in pediatric recipients of allogeneic hematopoietic stem cell transplantation (HSCT) a comparison between two methods: Real-time PCR (qPCR) vs. variable number tandem repeats PCR (VNTR PCR). Chimerism. 2013;4:1–9.

    Article  Google Scholar 

  6. 6.

    George D, Czech J, John B, Yu M, Jennings LJ. Detection and quantification of chimerism by droplet digital PCR. Chimerism. 2013;4:102–8.

    Article  Google Scholar 

  7. 7.

    Stahl T, Rothe C, Böhme MU, Kohl A, Kröger N, Fehse B. Digital PCR panel for sensitive hematopoietic chimerism quantification after allogeneic stem cell transplantation. Int J Mol Sci. 2016;17:102–8.

    Article  Google Scholar 

  8. 8.

    Australian Government Department of Health. National Pathology Accreditation Advisory Council (NPAAC). NPAAC Secretariat. 2015.

  9. 9.

    Stahl T, Böhme MU, Kröger N, Fehse B. Digital PCR to assess hematopoietic chimerism after allogeneic stem cell transplantation. Exp Hematol. 2015;43:462–8.e1.

    CAS  Article  Google Scholar 

  10. 10.

    Han J, Sun J, Zhao L, Zhao W, Liu Y, Li C. Validation study of a 15-plex rapid STR amplification system for human identification. Forensic Sci Int Genet. 2017;28:71–81.

    CAS  Article  Google Scholar 

  11. 11.

    Waterhouse M, Kunzmann R, Torres M, Bertz H, Finke J. An internal validation approach and quality control on hematopoietic chimerism testing after allogeneic hematopoietic cell transplantation. Clin Chem Lab Med. 2013;51:363–9.

    CAS  Article  Google Scholar 

  12. 12.

    Lion T, Watzinger F, Preuner S, Kreyenberg H, Tilanus M, De Weger R, et al. The EuroChimerism concept for a standardized approach to chimerism analysis after allogeneic stem cell transplantation. Leukemia. 2012;26:1821–8.

    CAS  Article  Google Scholar 

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Acknowledgements

We are grateful to Catherine O’Dwyer for guidance on quality controls and accredited requirements, and thanks to the transplantation team at the Alfred Hospital.

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Correspondence to Tongted P. Das.

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Das, T.P., Kipp, D.A., Kliman, D.S. et al. Important factors in implementation of lineage-specific chimerism analysis for routine use. Bone Marrow Transplant (2020). https://doi.org/10.1038/s41409-020-01089-6

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