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CRISPR/Cas9-modified hematopoietic stem cells—present and future perspectives for stem cell transplantation

Bone Marrow Transplantation (2019) | Download Citation


Allogeneic hematopoietic stem cell transplantation (HSCT) is a standard therapeutic intervention for hematological malignancies and several monogenic diseases. However, this approach has limitations related to lack of a suitable donor, graft-versus-host disease and infectious complications due to immune suppression. On the contrary, autologous HSCT diminishes the negative effects of allogeneic HSCT. Despite the good efficacy, earlier gene therapy trials with autologous HSCs and viral vectors have raised serious safety concerns. However, the CRISPR/Cas9-edited autologous HSCs have been proposed to be an alternative option with a high safety profile. In this review, we summarized the possibility of CRISPR/Cas9-mediated autologous HSCT as a potential treatment option for various diseases supported by preclinical gene-editing studies. Furthermore, we discussed future clinical perspectives and possible clinical grade improvements of CRISPR/cas9-mediated autologous HSCT.

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This work was financed by the research funding programs Jürgen Manchot Stiftung, Fortüne Tübingen (N°. 2412-0-0; N°. 2485-0-0), and the University Children’s Hospital of Tübingen.

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  1. These authors contributed equally: Alberto Daniel-Moreno, Andrés Lamsfus-Calle


  1. University Children’s Hospital, Department of Pediatrics I, Hematology and Oncology, University of Tübingen, Tübingen, Germany

    • Alberto Daniel-Moreno
    • , Andrés Lamsfus-Calle
    • , Janani Raju
    • , Justin S. Antony
    • , Rupert Handgretinger
    •  & Markus Mezger


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