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Epithelial barrier dysfunction as permissive pathomechanism in human intestinal graft-versus-host disease

Bone Marrow Transplantationvolume 53pages10831086 (2018) | Download Citation

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These authors contributed equally: Kathrin Rieger, Jörg-Dieter Schulzke

References

  1. 1.

    Gallucci BB, Sale GE, McDonald GB, Epstein R, Shulman HM, Thomas ED. The fine structure of human rectal epithelium in acute graft-versus-host disease. Am J Surg Pathol. 1982;6:293–305.

  2. 2.

    Ferrara JL, Levine JE, Reddy P, Holler E. Graft-versus-host disease. Lancet. 2009;373:1550–61.

  3. 3.

    Heidt PJ, Vossen JM. Experimental and clinical gnotobiotics: influence of the microflora on graft-versus-host disease after allogeneic bone marrow transplantation. J Med. 1992;23:161–73.

  4. 4.

    Stockmann M, Gitter AH, Sorgenfrei D, Fromm M, Schulzke JD. Low edge damage container insert that adjusts intestinal forceps biopsies into Ussing chamber systems. Pflug Arch. 1999;438:107–12.

  5. 5.

    Rosenthal R, Milatz S, Krug AM, Oelrich B, Schulzke JD, Amasheh S, et al. Claudin-2, a component of the tight junction, forms a paracellular water channel. J Cell Sci. 2010;123:1913–21.

  6. 6.

    Amasheh S, Meiri N, Gitter AH, Schöneberg T, Mankertz J, Schulzke JD, et al. Claudin-2 expression induces cation- selective channels in tight junctions of epithelial cells. J Cell Sci. 2002;115:4969–76.

  7. 7.

    Heller F, Florian P, Bojarski C, Richter J, Christ M, Hillenbrand B, et al. Interleukin-13 is the key effector Th2 cytokine in ulcerative colitis that affects epithelial tight junctions, apoptosis, and cell restitution. Gastroenterology. 2005;129:550–64.

  8. 8.

    Mankertz J, Amasheh M, Krug SM, Fromm A, Amasheh S, Hillenbrand B, et al. TNFalpha up-regulates claudin-2 expression in epithelial HT-29/B6 cells via phosphatidylinositol-3-kinase signaling. Cell Tissue Res. 2009;336:67–77.

  9. 9.

    Brown GR, Lindberg G, Meddings J, Silva M, Beutler B, Thiele D. Tumor necrosis factor inhibitor ameliorates murine intestinal graft-versus-host disease. Gastroenterology. 1999;116:593–601.

  10. 10.

    Jordan WJ, Brookes PA, Szydlo RM, Goldman JM, Lechler RI, Ritter MA. IL-13 production by donor T cells is prognostic of acute graft-versus-host disease following unrelated donor stem cell transplantation. Blood. 2004;103:717–24.

  11. 11.

    Amasheh S, Schmidt T, Mahn M, Florian P, Mankertz J, Tavalali S, et al. Contribution of claudin-5 to barrier properties in tight junctions of epithelial cells. Cell Tissue Res. 2005;321:89–96.

  12. 12.

    Zeissig S, Bürgel N, Günzel D, Richter J, Mankertz J, Wahnschaffe U, et al. Changes in expression and distribution of claudin 2, 5 and 8 lead to discontinuous tight junctions and barrier dysfunction in active Crohn’s disease. Gut. 2007;56:61–72.

  13. 13.

    Gitter AH, Wullstein F, Fromm M, Schulzke JD. Epithelial barrier defects in ulcerative colitis: characterization and quantification by electrophysiological imaging. Gastroenterology. 2001;121:1320–8.

  14. 14.

    Cooke KR, Gerbitz A, Crawford JM, Teshima T, Hill GR, Tesolin A, et al. LPS antagonism reduces graft-versus-host disease and preserves graft-versus-leukemia activity after experimental bone marrow transplantation. J Clin Invest. 2001;107:1581–9.

  15. 15.

    Wallon C, Braaf Y, Wolving M, Olaison G, Söderholm JD. Endoscopic biopsies in Ussing chambers evaluated for studies of macromolecular permeability in the human colon. Scand J Gastroenterol. 2005;40:586–95.

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Acknowledgements

We thank the team of the endoscopy unit (Campus Benjamin Franklin, Charité .- Universitätsmedizin Berlin) for the excellent cooperation and in memoriam D. Sorgenfrei for his remarkable technical assistance in the lab. This work was supported by grants from the Deutsche Forschungsgemeinschaft (DFG): Schu 559/11-2

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Affiliations

  1. Department of Gastroenterology, Infectiology and Rheumatology, Campus Benjamin Franklin, Charité - Universitätsmedizin Berlin, Berlin, Germany

    • Hanno Troeger
    • , Christian Bojarski
    •  & Britta Siegmund
  2. Department of General, Visceral and Vascular Surgery, Campus Benjamin Franklin, Charité - Universitätsmedizin Berlin, Berlin, Germany

    • Nina A. Hering
  3. Institute of Clinical Physiology, Campus Benjamin Franklin, Charité - Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, Berlin, Germany

    • Anja Fromm
    • , Christian Barmeyer
    • , Michael Fromm
    •  & Jörg-Dieter Schulzke
  4. Department of Hematology, Campus Benjamin Franklin, Charité - Universitätsmedizin Berlin, Berlin, Germany

    • Lutz Uharek
    •  & Kathrin Rieger

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Conflict of interest

The authors declare that they have no conflict of interest.

Corresponding author

Correspondence to Jörg-Dieter Schulzke.

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DOI

https://doi.org/10.1038/s41409-018-0144-2