Fig. 1: Rac1 is overexpressed in human primary MCL tumors and MCL cell lines. | Blood Cancer Journal

Fig. 1: Rac1 is overexpressed in human primary MCL tumors and MCL cell lines.

From: Rac1 is a novel therapeutic target in mantle cell lymphoma

Fig. 1

Analysis of Rac1 mRNA levels in (a) human primary MCL tissues from the LLMPP database and (b) MCL cell lines. c Upper panel: Rac1-GTP, Rac1-total and β-actin protein expression in MCL cell lines was analyzed by western blot; lower panel: quantification of Rac1-GTP level by Odyssey CLx system (LI-COR). This calculation was based on the ratio between Rac1-GTP signal and that of Rac1-total. The experiments exhibited in (b) and (c) were repeated three times, and an average ratio to that of the naive B cells is shown. dg Representative images of the immunohistochemistry (IHC) for tonsil (d) and MCL lymphoma cases that is negative for Rac1 (e), positive for Rac1 (f, g, W weak Rac1 staining, S strong Rac1 staining). h Overall survival of MCL patients in relation to Rac1 protein expression

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