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Characterization of LTr1 derived from cruciferous vegetables as a novel anti-glioma agent via inhibiting TrkA/PI3K/AKT pathway

Acta Pharmacologica Sinica volume 44, pages 1262–1276 (2023)Cite this article

Abstract

Malignant glioma is the most fatal, invasive brain cancer with limited treatment options. Our previous studies show that 2-(indol-3-ylmethyl)-3,3′-diindolylmethane (LTr1), a major metabolite of indole-3-carbinol (I3C) derived from cruciferous vegetables, produces anti-tumour effect against various tumour cell lines. In this study we characterized LTr1 as a novel anti-glioma agent. Based on screening 134 natural compounds and comparing the candidates’ efficacy and toxicity, LTr1 was selected as the lead compound. We showed that LTr1 potently inhibited the viability of human glioma cell lines (SHG-44, U87, and U251) with IC50 values of 1.97, 1.84, and 2.03 μM, respectively. Furthermore, administration of LTr1 (100,300 mg· kg−1 ·d−1, i.g. for 18 days) dose-dependently suppressed the tumour growth in a U87 xenograft nude mouse model. We demonstrated that LTr1 directly bound with TrkA to inhibit its kinase activity and the downstream PI3K/AKT pathway thus inducing significant S-phase cell cycle arrest and apoptosis in SHG-44 and U87 cells by activating the mitochondrial pathway and inducing the production of reactive oxygen species (ROS). Importantly, LTr1 could cross the blood-brain barrier to achieve the therapeutic concentration in the brain. Taken together, LTr1 is a safe and promising therapeutic agent against glioma through inhibiting TrkA/PI3K/AKT pathway.

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Fig. 1: Screening of lead compounds for anti-glioma activity.
Fig. 2: LTr1 inhibited the proliferation of glioma cells via inducing S phase arrest.
Fig. 3: LTr1 induced glioma cells apoptosis via activating the mitochondrial intrinsic apoptotic pathway.
Fig. 4: LTr1 induced oxidative damage in glioma cells.
Fig. 5: TrkA was a potential target for glioma.
Fig. 6: LTr1 was a TrkA inhibitor.
Fig. 7: LTr1 inhibited tumour growth and induced cell apoptosis in U87- subcutaneously heterotopic xenografted nude mice.
Fig. 8: LTr1 inhibited the growth and metastasis of tumour in U87 orthotopic xenograft model.

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Acknowledgements

This research was supported by grants from the National Key R&D Program of China (Nos. 2021ZD0202901 and 2018YFC1706205), and the National Natural Science Foundation of China (Nos. 81991523 and 82073721). We thank the Experiment Center for Science and Technology, Nanjing University of Chinese Medicine, for providing the instrumentation.

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  1. These authors contributed equally: Qi-qi Song, Li-ping Lin

Authors and Affiliations

  1. Departments of Pharmacology, School of Medicine and Holistic Integrative Medicine, Nanjing University of Chinese Medicine, Nanjing, 210023, China

    Qi-qi Song, Ya-li Chen, Ke Wei, Jian-wei Su, Yang Liu & Gang Hu

  2. State Key Laboratory Cultivation Base for TCM Quality and Efficacy, Nanjing University of Chinese Medicine, Nanjing, 210023, China

    Li-ping Lin, Jia-cheng Qian & Ren-xiang Tan

  3. Jiangsu Key Laboratory of Neurodegeneration, Department of Pharmacology, Nanjing Medical University, Nanjing, 211100, China

    Jian-hua Ding, Ming Lu & Gang Hu

  4. State Key Laboratory of Pharmaceutical Biotechnology, Institute of Functional Biomolecules, Nanjing University, Nanjing, 210023, China

    Ren-xiang Tan

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Contributions

GH, RXT, and YL designed the experiments. QQS, LPL, JCQ, YLC, KW, and JWS performed the experiments. QQS, LPL, and YL analyzed and interpreted the data from experiments. JHD provided technical support. QQS wrote the manuscript. YL, ML, and LPL made critical modifications to the manuscript. All authors read and approved the final manuscript.

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Correspondence to Yang Liu, Ren-xiang Tan or Gang Hu.

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Song, Qq., Lin, Lp., Chen, Yl. et al. Characterization of LTr1 derived from cruciferous vegetables as a novel anti-glioma agent via inhibiting TrkA/PI3K/AKT pathway. Acta Pharmacol Sin 44, 1262–1276 (2023). https://doi.org/10.1038/s41401-022-01033-y

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