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Plasma miR-142 predicts major adverse cardiovascular events as an intermediate biomarker of dual antiplatelet therapy

Abstract

MicroRNAs (miRNAs) are widely expressed in organisms and are implicated in the regulation of most biological functions. The present study investigated the association of plasma miRNAs with the clinical outcomes of dual antiplatelet therapy in coronary artery disease (CAD) patients who underwent percutaneous coronary intervention (PCI). Plasma miRNA levels were screened using high-throughput Illumina sequencing to evaluate the antiplatelet efficacy of clopidogrel and aspirin. Six plasma miRNAs (miR-126, miR-130a, miR-27a, miR-106a, miR-21, and miR-142) were associated with clopidogrel-treated platelet aggregation. These miRNAs were validated in a prospective cohort of 1230 CAD patients using quantitative reverse transcription-polymerase chain reaction (qRT-PCR). High plasma miR-142 levels were associated with a high risk of major adverse cardiovascular events (MACE), with a hazard ratio (95% confidence interval) of 1.83 (1.30–2.59) at a false discovery rate of <5%. Multivariable Cox regression analysis revealed that diabetes mellitus, heart failure, calcium channel blocker application, and a high plasma miR-142 level were independent risk factors of MACE. The levels of the six plasma miRNAs were not significantly associated with bleeding events during the 3-year follow-up. In conclusion, plasma miR-142 is potential marker to predict MACE in CAD patients after PCI.

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The authors declare no competing interests

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Acknowledgements

This work was supported by the National Key R&D program (No 2017YFC0909301 and 2016YFC0905003), National Natural Science Foundation of China (No 81673514, 81373486, 81202602, and 81470440), Science and Technology Development Projects of Guangdong Province, China (No 2016B090918114), and Science and Technology Development Projects of Guangzhou, Guangdong, China (201510010236 and 201604020096).

Authors contributions

SZ, YH, QT, HL, and HW designed the research. QT, HL, and HW performed the experiments and wrote the article. SZ, YH, and QT analyzed the data. Other colleagues helped collect the sample and clinical data. All authors have read and approved the final manuscript.

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Competing interests

The authors declare no competing interests

Correspondence to Ya-ling Han or Shi-long Zhong.

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Keywords

  • plasma miRNAs
  • miR-142
  • antiplatelet therapy
  • MACEs
  • coronary artery disease
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