Introduction

Clinical studies suggest that psilocybin, and active metabolite psilocin [1,2,3], produce rapid, long-lasting improvements in psychiatric disorders, including depression, anxiety, and substance use disorder [4,5,6,7,8,9,10,11,12]. Sustained improvements in symptoms were seen as long as 4 years following administration [13]. Research on psychedelic compounds has focused primarily on human imaging studies and receptor pharmacology. Human functional magnetic resonance imaging (fMRI) studies have shown that psychedelics produce robust alterations in network connectivity [8, 14,15,16,17,18,19]. Receptor studies have demonstrated that these compounds promote synaptogenesis [20,21,22] and identified the serotonin 5-hydroxytryptamine (5-HT)2A receptor to be instrumental in mediating the hallucinogenic actions of psychedelic compounds, including psilocybin and lysergic acid diethylamide (LSD) [16, 23,24,25,26,27,28]. Despite the observed clinical outcomes in psychedelic studies, the underlying neural correlates of these therapeutic effects remain relatively unknown.

The central amygdala (CeA) is a primary output region within the extended amygdala that receives input regarding internal and external arousal state and coordinates appropriate behavioral responses [29, 30]. CeA dysregulation has been implicated in anxiety, depression, and PTSD [29, 31,32,33,34,35,36]. Preclinical studies demonstrate that the psychedelic compounds LSD and 2,5-dimethoxy-4-iodoamphetamine (DOI) increase Fos expression in the amygdala [37, 38] and that systemic and local DOI administration in the amygdala promotes suppression of fear responses [38]. Psilocybin produces alterations in reactivity and connectivity of the amygdala that correlate with positive therapeutic outcome [12, 15, 17, 39,40,41]. One clinical imaging study reported significantly decreased amygdala reactivity 1-week following psilocybin administration, with lasting changes in affect at 1-month follow-up [41].

The current study utilized a preclinical approach to determine how the active compound, psilocin, alters CeA activity, reactivity, and behavioral responding to an aversive stimulus in male and female Sprague Dawley rats. Psilocin was used as it is the active metabolite of psilocybin in the central nervous system (CNS), mediating effects of psilocybin at 5-HT2AR [2, 28]. We focused on the CeA as it’s the primary output region of the amygdala and as amygdala hyper-reactivity is associated with many psychiatric disorders that psilocybin shows promise in treating [42,43,44,45]. We first measured expression of c-Fos, an immediate early gene that is upregulated following neuronal activity, in the CeA of male and female rats administered psilocin (2 mg/kg) or vehicle. Next, we measured CeA reactivity using an air-puff stimulus involving a burst of air directed towards the face. The air-puff stimulus has been validated as an aversive stimulus in passive avoidance tests [46] and, due to known CeA involvement in pain modulation [47, 48], it was essential to utilize a stimulus that would evoke a response without the addition of potential confounding effects of pain. Utilizing fiber photometry in conjunction with the aversive air-puff stimulus, we were able to demonstrate a robust and reliable CeA reactivity response. We then used this model to determine how administration of psilocin altered CeA reactivity during acute exposure and at more prolonged timepoints. We observed sex-specific effects of psilocin on CeA activity, as measured by c-Fos expression, and in vivo reactivity and behavioral responding. Specifically, we found that psilocin elicited acute increases in reactivity in females, but not males. Additionally, we found long-term decreases in CeA reactivity in males that were not seen in females or in the vehicle control. To test whether these effects would contribute to the development of a conditioned response to the sound of the air-puff, additional assays were performed utilizing an auditory-only air-puff stimulus, wherein an air-puff was administered with the nozzle facing outside of the box to isolate the auditory component of the air-puff. In doing so, we show differential reactivity of the CeA suggesting that the observed changes were stimulus-specific and dynamic in nature. Finally, we found that decrease in reactivity in males were primarily driven by animals that exhibited darting vs. freezing behavior in response to the air-puff stimulus.

Methods

Animals/stereotaxic surgery

Adult male and female Sprague Dawley rats (200–400 g, ~7 weeks old at arrival, Envigo, Indianapolis, IN) were group-housed in a humidity- and temperature-controlled (22 °C) vivarium on a 12 h light/dark cycle with ad libitum access to food and water. Bilateral infusions of pGP-AAV-syn-jGCaMP7f-WPRE (Addgene plasmid# 104488-AAV9) were performed at a rate of 100 nl/minute into the CeA (AP:−2.0 mm; ML: +/−3.9 mm; DV: −8.0 mm). Directly following injection, Doric 200 μm NA 0.37 silica optic fiber cannulas with a 9 mm tip were inserted at DV −7.7 mm and secured in place using Metabond (Parkell, Brentwood, NY).

Following intracranial surgery at ~8 weeks of age, animals were single-housed in flat lid cages with waterspout access, and food bowls to avoid damage to implants. All animal protocols were approved by the Animal Care and Use Committee at the University of North Carolina at Chapel Hill.

Drug administration

On the day of injection, animals received a subcutaneous (s.c.) injection of either vehicle (0.9% Saline/2% glacial acetic acid) or a 2 mg/kg psilocin (Cayman Chemical, graciously provided by Dr. Bryan Roth) dissolved in 2% glacial acetic acid, as previously described [49]. Solutions were prepared fresh on the day of testing and were stored at 4 °C for 30 min prior to first injection. Injections were given at a 1 mg/ml volume 40 min prior to the beginning of fiber photometry recordings. In the c-Fos cohort, injections were performed 2 h prior to perfusion. For this study, a dose of 2 mg/kg was chosen as previous work has shown that administration of psilocin at 2 mg/kg in rats produced increases in BOLD signaling in the amygdala, an effect not seen when utilizing a lower dose [50].

Fiber photometry recording

Sample sizes were determined based on pilot experiments along with referencing sample sizes of related studies in the literature [51, 52]. All animals were ~11 weeks of age at the beginning of testing. Animals were habituated to testing room for 1 h prior to recording sessions. Rats were individually placed in a clear plexiglass chamber [50 cm (l) × 50 cm (w) × 38 cm (h)] in a red-light sound-attenuated behavioral cabinet and allowed to acclimate for 10 min. Fiber placement and GCaMP injection were performed bilaterally, however, recordings were collected unilaterally. Baseline recordings were taken from both hemispheres and analyzed to determine which hemisphere to use for the remainder of the experiment. Ca2+ signals from subjects, with 200 μm optic fibers placed 0.3 mm above the injection site in the CeA, were recorded using a TDT RZ5 real-time processor equipped with the TDT Synapse program (Tucker-Davis Technologies, Alachua, FL). For each recording, jGCaMP7f was excited using a 465 nm calcium-dependent signal and a 405 nm signal was used as an isosbestic control. MATLAB script [51] was then used to analyze raw signal. Changes in fluorescence as a function of baseline (ΔF/F) showing fluctuations in fluorescence compared to the overall baseline fluorescence throughout the session were calculated through smoothing isosbestic control channel signal, scaling the isosbestic signal by regressing it onto smoothed GCaMP signal, and then generating a predicted 405 nm from the regression to remove calcium-independent signaling from the raw GCaMP signal and control for artifact from movement, photo-bleaching, and fiber bending artifacts. Baseline fluorescence was calculated through a least-squares linear fitting of the 405 nm isosbestic control to the 465 nm GCaMP signal to create a resting GCaMP signal over the entire session not containing event-dependent fluctuations in GCaMP signal [53]. Peri-event plots were then generated by averaging changes in fluorescence (ΔF/F) across two different air-puff trials to generate a mean change in reactivity [51]. For all photometry experiments, the same male experimenter handled animals and recording and was not blinded during recording sessions. Additionally, female animals were not checked for estrus cycle to avoid potential confounding effects of an added stressor. All subjects were video recorded during stimulus presentations and behavior was scored using the Behavioral Observation Research Interactive Software (BORIS) program [54]. Behavioral analysis was performed by an experimenter blind to the experimental status of each animal. Animals were randomly assigned into groups by subject number following surgery.

Air-puff stimulus

An aversive air-puff stimulus was designed using house air supply (85 psi) controlled by a Parker solenoid (Part #003-0868-900), powered by a custom made 12 V electrical circuit box connected to the TDT RZ5 system to trigger 500 ms openings of the solenoid and simultaneous timestamps through the Synapse program. Each session consisted of a 10 min habituation period before recording sessions that consisted of two air-puff administrations with a 5 min inter-stimulus interval (ISI) between them. Air-puffs were directed at the face and within close proximity of the animal to maintain similar conditions and level of aversion to the stimulus between subjects.

Perfusion/tissue collection

Rats were transcardially perfused with phosphate-buffered saline (PBS) followed by 4% paraformaldehyde (w/v). Brains were extracted, stored in 4% paraformaldehyde for 24 h, then transferred to 30% sucrose (w/v) and stored at 4 °C until sectioning. Using a freezing microtome, tissue was sliced into 40 µm-thick sections and stored in cryoprotectant (30% v/v ethylene glycol + 30% w/v sucrose in phosphate buffered saline) at 4 °C until use.

Immunofluorescence staining

For the c-Fos cohort, sections (AP coordinates −1.92 mm to −2.16 mm) were washed in PBS, incubated in 50% methanol/PBS solution for 30 min, washed in 3% hydrogen peroxide/PBS for 5 min, placed in blocking solution (0.3% Triton X-100; Thermo Fisher), and then 1% bovine serum albumin (BSA; Sigma) for 1 h, at room temperature (RT). Slices were then incubated with rabbit anti-cFos (1:3000, Millipore Sigma; ABE457) for 24 h at 4 °C. Next, slices were washed with 0.1% Tween-20 in tris-buffered saline (TNT) before being transferred into TNB blocking buffer (Perkin-Elmer FP1012) for 30 min. After blocking, slices were incubated in goat anti-rabbit horseradish peroxidase (HRP; 1:200, Abcam ab6721) for 2 h followed by another round of TNT washes. Finally, slices were incubated in tyramide-conjugated fluorescein (1:50) in TSA amplification diluent (Akoya Biosciences, NEL741001KT) for 10 min at RT. Slices were washed with TNT buffer, mounted, cover slipped with Vectashield ® HardSet™ Antifade Mounting Medium with DAPI (H1500, Vector Laboratories, Burlingame, CA) and stored at 4 °C before being imaged with the Keyence BZ-X800 fluorescence microscope. Images were then analyzed by an experimenter blinded to the animal’s condition. Images were taken at ×20 and the BZ-X800 Analyzer program was used to stitch images together. Randomly selected hemispheres were used, and the area of interest was outlined and manually counted using the ImageJ [55] multi-point counter tool. Cell counts were averaged per animal.

Statistical analysis

Raw data from the TDT RZ5 machine were imported and processed for artifact removal, down sampling, and detrending using MATLAB code [51]. Changes in fluorescence (ΔF/F) were then calculated and collected 5 s before and 10 s following air-puff administration. The mean of two traces were averaged together for each subject and combined using custom MATLAB script (https://doi.org/10.5061/dryad.pnvx0k6q9) to sort individual data into groups. Group 465 nm signal data were then converted into 500 ms bins for analysis to quantify differences between experimental conditions. All binned trace plots were normalized by subtracting the mean of the signal pre-air-puff from each time point in order to correct for differences in basal activity prior to air-puff administration. All comparisons were made using time bins post air-puff, corresponding to 0–20 on the x-axis of binned analysis plots. All statistical analyses were conducted using GraphPad Prism 7. Five subjects were removed from the study due to lack of signal, misplaced cannula, or cannula removal. For the female groups, a repeated-measures one-way analysis of variance (ANOVA) with Dunnett’s post hoc test was used to determine differences between baseline, injection day, and follow-up time points within each group in the air-puff condition. All data points in the female groups met normality and variance assumptions. For the male vehicle and psilocin groups, a Friedman test with Dunn multiple comparison was performed as not all time points met normality assumptions. Two-way ANOVAs with Šidák’s post hoc tests were used to compare time bins between groups and time points. All data points met normality assumption for the two-way ANOVA. Variance in ANOVAs was not assumed, and Geisser–Greenhouse epsilon values were reported where appropriate and were never below the lower bound threshold for epsilon of 1/(n−1). Paired t tests were used to determine differences within groups between the peak point between the initial and final auditory only recording days. Fisher’s exact tests were used to compare percentages in darting behavior. Mixed-effects two-way ANOVA were used to compare time spent walking and rearing. For all binned trace data plots, a bootstrapping confidence interval (CI) procedure (99% CI, 1000 iterations) [56] was conducted to calculate a new “mean” ΔF/F by randomly resampling from subject mean ΔF/F with replacement corresponding to the number of samples. The n for all analyses corresponded to the number of subjects in each group. This process was repeated 1000 times to create a nonparametric distribution of population mean simulations for each time point within the window. A statistically significant increase (>0%) in Ca2+ transients following the air-puff administration was defined as those data points wherein the lower bound of the 99% CI was >0 [56,57,58]. This was signified on graphs by color matched lines above the trace bin plots. All data including Matlab structures and scripts can be found at https://doi.org/10.5061/dryad.pnvx0k6q9.

Results

Acute changes in CeA reactivity in response to an aversive air-puff stimulus

At ~8 weeks, male and female Sprague Dawley rats received bilateral injection of pGP-AAV-syn-jGCaMP7f-WPRE into the CeA immediately followed by installation of fiber optic cannulae above the injection site (Fig. 1A, B). Following 3 weeks for virus transduction, animals underwent a series of fiber photometry experiments testing the effects of psilocin on fluctuations in calcium dynamics within the CeA time-locked to an aversive air-puff stimulus (Fig. 1A). For both baseline and injection recording sessions, each animal received two air-puffs with an ISI of 5 min and trace plots were generated showing change in fluorescence as a function of baseline (ΔF/F). Traces were analyzed 5 s before and 10 s following administration of air-puff (Fig. 1C). Following injection of the drug, animals were not assessed for any behavioral indices of hallucination (i.e., head twitch, wetback shake).

Fig. 1: Schematic including experimental timeline, representative coronal image, and group mean photometry traces.
figure 1

A Experimental timeline. Animals arrived at 6–7 weeks old and were allowed to habituate for 1 week prior to surgeries. Bilateral injection of calcium sensor (jGCaMP7f) and implantation of fiber optic cannulas were conducted. After 3 weeks for viral transduction, animals underwent a series of fiber photometry experiments. B Representative coronal section showing fiber placement and GCaMP expression. C Mean air-puff traces showing consistency of CeA signal in response to the air-puff stimulus.

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Sub-region-specific differences in CeA basal activity following psilocin administration

To assess the effects of psilocin on basal activity in the CeA, male and female Sprague Dawley rats were injected with psilocin (2 mg/kg, s.c.) or vehicle (1 ml/kg, s.c.) 2 h prior to transcardial perfusion. Immunohistochemistry (IHC) assays were performed using CeA tissue collected within the anterior-posterior bregma range of −1.92 mm to −2.16 mm. In females, there was a significantly increased c-Fos expression in the psilocin group as compared to vehicle across regions (2-way ANOVA: FInteraction(2,24)=1.597, p = 0.22; FRegion(1,24)=30.84, p < 0.0001; Finjection(1,24)=9.149 p = 0.006; Fig. 2A, B) with greater increases in the capsular division of the central amygdala (CeC) (Šidák’s: p = 0.02). In males, there was also significantly increased c-Fos expression in the psilocin group across regions (2-way ANOVA: FInteraction(2,27)=1.241, p = 0.30; FRegion(2,27)=12.02, p = 0.0002; Finjection(1,27)=5.543, p = 0.02; Fig. 2C, D).

Fig. 2: Acute changes in CeA reactivity in response to an aversive air-puff stimulus following psilocin administration.
figure 2

A Females: representative images showing c-Fos+ cells tagged with a green fluorescence protein (GFP) in the CeA. Scale bar = 100 μm. B Female vehicle vs. psilocin: histogram showing the number of c-Fos+ cells in each subregion of the CeA. CeC capsular CeA, CeM Medial CeA, CeL lateral CeA. Data points correspond to the mean of 2–4 hemispheres for each individual subject. C Males: representative images showing c-Fos+ cells tagged with a green fluorescence protein (GFP) in the CeA. Scale bar = 100 μm. D Male vehicle vs. psilocin: histogram showing the number of c-Fos+ cells in each subregion of the CeA. CeC capsular CeA, CeM medial CeA, CeL lateral CeA. Data points correspond to the mean of 2–4 hemispheres for each individual subject. E Female psilocin group: representative raw ΔF/F traces showing an individual at baseline and while on psilocin. Blue line = air-puff onset, Gray lines = individual traces, Green line = mean trace, Std standard deviation. F Females: Heatmaps comparing baseline to injection. Each row represents an individual subjects mean trace. G Female vehicle group: air-puff trace plots of changes in CeA fluorescence following exposure to a 500 ms air-puff at 85 psi. Data points represent group averages within 500 ms binned window +/− S.E.M. H Female psilocin group: air-puff trace plots of changes in CeA fluorescence following exposure to a 500 ms air-puff at 85 psi. Data points represent group averages within 500 ms binned window +/− S.E.M. I Male psilocin group: representative raw ΔF/F traces showing an individual at baseline and while on psilocin. Blue line = air-puff onset, Gray lines = individual traces, Green line = mean trace, Std standard deviation. J Males: Heatmaps comparing baseline to injection. Each row represents an individual subjects mean trace. K Male vehicle group: air-puff trace plots of changes in CeA fluorescence following exposure to a 500 ms air-puff at 85 psi. Data points represent group averages within 500 ms binned window +/− S.E.M. L Male psilocin group: air-puff trace plots of changes in CeA fluorescence following exposure to a 500 ms air-puff at 85 psi. Data points represent group averages within 500 ms binned window +/− S.E.M. In each trace bin plot panel, a significant increase in ΔF/F was determined whenever the lower bound of the 99% CI was >0. These points of statistical significance are shown as colored lines above each ΔF/F curve with colors corresponding to the respective binned traces with a * above the lines. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.

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Acute changes in CeA reactivity in response to an aversive air-puff stimulus following psilocin administration

To test the acute effects of psilocin on CeA reactivity, we measured changes in CeA reactivity during time-locked stimuli presentation at baseline and 40 min post-administration of vehicle or psilocin (2 mg/kg, s.c.). Following a 10 min habituation period, air-puffs were delivered as described above. Mean traces were taken from two separate air-puff responses within a session (Fig. 2E, I). These traces were then collapsed into 500 ms bins for statistical analysis (Fig. 2G, H, K, L). A repeated measures one-way ANOVA or Friedman test was used to determine an effect of all the different timepoints (baseline, injection, 2 days, 6 days, and 28 days) within each group depending on normality. A bootstrapping 99% confidence interval (CI) procedure was used to estimate population mean ΔF/F during the 10 time bins (5 s) prior and 20 time bins (10 s) following air-puff administration. A significant increase in ΔF/F from 0 to 20 time bins (0–10 s) following air-puff was determined whenever the lower bound of the 99% CI was >0. These points of statistical significance are shown above binned trace ΔF/F plots and are colored coded to match the corresponding time bin trace. In the female vehicle control group (n = 9), there was a main effect of time points (F(1.558,29.61)=29.32, ε = 0.39, p < 0.0001). However, there were no changes between baseline and injection (p = 0.36; Fig. 2F, G). In the female psilocin group (n = 7), there was a significant effect of time points (F(3.178,60.38)=50.25, ε = 0.79, p < 0.0001) with a significant increase between baseline and injection (p < 0.0001; Fig. 2F, H). In the male vehicle control group (n = 10), there was a significant difference between time points (X2(4)=36.92, ε = 0.45, p < 0.0001) with an increase in reactivity between baseline and injection (p = 0.0006; Fig. 2J, K), though this effect appears to be primarily driven by a single subject as opposed to uniform increases across all subjects (Fig. 2J). In the male psilocin group (n = 11), there was a significant difference between time points (X2(4)=58.48, ε = 0.54, p < 0.0001) with no significant difference between baseline and injection (p > 0.999; Fig. 2J, L).

Prolonged changes in CeA reactivity in response to an aversive air-puff stimulus following psilocin administration

To assess long-term alterations in CeA reactivity following a single dose of psilocin, fiber photometry recordings were performed on days 2, 6, and 28 post-administration of vehicle or psilocin. On each follow-up recording day, animals received two air-puffs with a 5-min ISI. In the vehicle control females, there was an effect of time points (F(1.558,29.61)=29.32, ε = 0.39, p < 0.0001; Fig. 3B, C), with significant increases in reactivity seen at the 2-day (p < 0.0001) and 28-day follow-up (p = 0.0005) compared to baseline. In the female psilocin group (Fig. 3A, D), there was an effect of time points (F(3.178,60.38)=50.25, ε = 0.79, p < 0.0001; Fig. 3B, D), with significant increases in reactivity at the 2-day (p = 0.02), 6-day (p < 0.0001), and 28-day follow-up (p = 0.04).

Fig. 3: Prolonged changes in CeA reactivity in response to an aversive air-puff stimulus following psilocin administration.
figure 3

A Female psilocin group: representative raw ΔF/F traces showing an individual at baseline and at the 28-day follow-up. Blue line = air-puff onset, Gray lines = individual traces, Green line = mean trace, Std standard deviation B Females: Heatmaps comparing baseline to the 28-day follow-up. Each row represents an individual subjects mean trace. C Female vehicle group: air-puff trace plots of changes in CeA fluorescence following exposure to a 500 ms air-puff at 85 psi. Data points represent group averages within 500 ms binned window +/− S.E.M. D Female psilocin group: air-puff trace plots of changes in CeA fluorescence following exposure to a 500 ms air-puff at 85 psi. Data points represent group averages within 500 ms binned window +/− S.E.M. E Male psilocin group: representative raw ΔF/F traces showing an individual at baseline and while on psilocin. Blue line = air-puff onset, Gray lines = individual traces, Green line = mean trace, Std standard deviation. F Males: Heatmaps comparing baseline to the 28-day follow-up. Each row represents an individual subjects mean trace. G Male vehicle group: air-puff trace plots of changes in CeA fluorescence following exposure to a 500 ms air-puff at 85 psi. Data points represent group averages within 500 ms binned window +/− S.E.M. H Male psilocin group: air-puff trace plots of changes in CeA fluorescence following exposure to a 500 ms air-puff at 85 psi. Data points represent group averages within 500 ms binned window. I Females: Summary histogram comparing the mean of all 20 time bins following air-puff administration. Each data point represents an individual time bin within that condition collapsed across all subjects. +/−S.E.M. J Males: Summary histogram comparing the mean of all 20 time bins following air-puff administration +/− S.E.M. Each data point represents an individual time bin within that condition collapsed across all subjects. +/−S.E.M. In each trace bin plot panel, a significant increase in ΔF/F was determined whenever the lower bound of the 99% CI was >0. These points of statistical significance are shown as colored lines above each ΔF/F curve with colors corresponding to the respective binned traces with a * above the lines. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.

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In the male vehicle group, there was a significant difference between time points (X2(4)=36.92, ε = 0.45, p < 0.0001; Fig. 3F, G), with a significant increase in reactivity at the 2-day follow-up (p = 0.0002). In the male psilocin group, there was a significant effect of time point (X2(4)=58.48, ε = 0.54, p < 0.0001; Fig. 3F, H), with significant decreases in reactivity seen at the 2-day (p = 0.01), 6-day (p < 0.0001), and 28-day follow-up (p = 0.001).

To compare changes in reactivity between vehicle and psilocin groups, 2-way ANOVA revealed differential effects of injection across time points between groups in females (FInteraction(4,152)=53.46, p < 0.0001; FInjection(1,38)=2.015, p = 0.16; FTime(2.343,89.04)=18.82, ε = 0.59, p < 0.0001; Fig. 3J) and males (FInteraction(4,152)=23.96, p < 0.0001; FInjection(1,38)=2.310, p = 0.13, FTime(2.603,98.90)=50.45, ε = 0.65, p < 0.0001; Fig. 3I). Šidák post hoc analysis revealed significant increases in reactivity during injection (p < 0.0001) and at the 2-day follow-up (p = 0.005) in the female psilocin group compared to vehicle control (Fig. 3J). In males, there was a significant decrease in reactivity at the 2-day follow-up in the psilocin group compared to vehicle control (p = 0.003; Fig. 3J)

Acute and prolonged behavioral effects of psilocin

To determine if psilocin elicited changes in locomotion and exploratory behavior, video recordings were scored for total time spent walking and time spent rearing, respectively. All behavior was scored during the 5 min ISI between the first and second air-puff. Rearing was defined as standing on the two hindlegs, regardless of any balancing on the walls of the box. In females, there were no differences in overall locomotion between vehicle and psilocin groups (FInteraction(3,42)=0.2118, p = 0.89; FInjection(1,14)=2.238, p = 0.16; FTime(2.497,34.95)=0.6065, ε = 0.83, p = 0.59; Fig. 4A). There were also no differences in rearing between female vehicle and psilocin groups (FInteraction(4,56)=1.345, p = 0.26; FInjection(1,14)=4.433, p = 0.05; FTime(4,56)=2.486, ε = 0.56, p = 0.05; Fig. 4B). In the males, there were no differences in locomotion (FInteraction(3,57)=0.1054, p = 0.96; FInjection(1,19)=2.563, p = 0.12; FTime(3,57)=1.255, ε = 0.78, p = 0.29; Fig. 4C) or rearing (FInteraction(4,76)=0.5546, p = 0.69; FInjection(1, 19)=0.7576, p = 0.39; FTime(4,76)=1.190, ε = 0.72, p = 0.32; Fig. 4D) between the vehicle and psilocin groups. Collectively, no significant alterations in locomotion were seen resulting from psilocin administration (Fig. 4A, C), although females appeared to display increased locomotion overall as compared to males, regardless of injection type.

Fig. 4: Acute and Prolonged Behavioral Effects of Psilocin.
figure 4

A Females: locomotion plots comparing vehicle and psilocin groups at each time point. Data points are mean time in seconds +/− S.E.M. B Females: time spent rearing comparing vehicle and psilocin groups at each time point. Data points are mean time in seconds +/− S.E.M. C Males: locomotion plots comparing vehicle and psilocin groups at each time point. Data points are mean time in seconds +/− S.E.M. D Males: time spent rearing comparing vehicle and psilocin groups at each time point. Data points are mean time in seconds +/− S.E.M. E Females threat responding behavior. First figure is showing percentage of subjects that darted following air-puff administration. Second figure is showing the percentage of animals that remained immobile following air-puff. Third and fourth figure are pie charts showing the percentage of animals that darted vs. remained immobile F Males threat responding behavior: First figure is showing percentage of subjects that darted following air-puff administration. Second figure is showing the percentage of subject that remained immobile following air-puff. Third and fourth figure are pie charts showing the percentage of animals that darted vs. remained immobile. G Male vehicle passive responders (remained immobile) group: First figure shows air-puff trace plots of changes in CeA fluorescence following exposure to a 500 ms air-puff at 85 psi. Data points represent group averages within 500 ms binned window +/− S.E.M.; second figure is a histogram showing differences in the mean peak ΔF/F value for each group +/− S.E.M following air-puff administration. Data points reflect each individual subject within the corresponding subgroup. H Male psilocin passive responders (remained immobile) group: First figure shows air-puff trace plots of changes in CeA fluorescence following exposure to a 500 ms air-puff at 85 psi. Data points represent group averages within 500 ms binned window +/− S.E.M. Second figure is a histogram showing differences in the mean peak ΔF/F value for each group +/− S.E.M following air-puff administration. Data points reflect each individual subject within the corresponding subgroup. I Male vehicle active responders (darted) group: First figure shows air-puff trace plots of changes in CeA fluorescence following exposure to a 500 ms air-puff at 85 psi. Data points represent group averages within 500 ms binned window +/− S.E.M. Second figure is a histogram showing differences in the mean peak ΔF/F value for each group +/− S.E.M following air-puff administration. Data points reflect each individual subject within the corresponding subgroup. J Male psilocin active responders (darted) group: First figure shows air-puff trace plots of changes in CeA fluorescence following exposure to a 500 ms air-puff at 85 psi. Data points represent group averages within 500 ms binned window +/− S.E.M. Second figure is a histogram showing differences in the mean peak ΔF/F value for each group +/− S.E.M following air-puff administration. Data points reflect each individual subject within the corresponding subgroup. *p < 0.05, **p < 0.01.

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To probe potential differences in threat responding to the air-puff stimulus, we separated groups based on active or passive coping strategy. Subgroups were created containing animals that remained immobile (passive) following air-puff administration versus animals that exhibited darting behavior (active) following air-puff administration at baseline. Consistent with previous literature [59], females primarily employed an active darting response (Fig. 4E), while active vs. passive coping behavior was more evenly split in males (Fig. 4F). In females, there were no differences in the percentage of animals that darted or that stayed immobile between groups at any of the time points (Fig. 4E). In contrast, the male psilocin group displayed nonsignificant increases in darting and reductions in immobility in the vehicle control group that were not seen in the psilocin group under injection conditions (Fisher’s exact: p = 0.08; Fig. 4F). At the 2-day follow-up, there was a significantly higher percentage of darting in the vehicle control group compared to the psilocin group (p = 0.03; Fig. 4F, left) and a significantly higher percentage immobile in the psilocin group (Fisher’s exact: p = 0.03; Fig. 4F, right). Though differences were not significant at every time point, psilocin seemed to prevent a trending increase in darting behavior seen in the male vehicle control group and promote passive responding in male but not female rats (Fig. 4F).

To explore the CeA reactivity correlates to the observed behavioral differences in threat responding, fiber photometry traces were split into the corresponding active vs. passive responder groups. Since females primarily employed active coping strategies that were not affected by psilocin, we only examined CeA reactivity between the male active and passive responders. To assess changes in the amplitude of CeA response to the stimulus, comparisons looking at the peak point, or highest ΔF/F value following air-puff, were made. In the male passive responder, there were no significant differences in peak point in the vehicle (F(4,20)=1.019, p = 0.42; Fig. 4G) or psilocin group (F(4,20)=1.278, p = 0.31; Fig. 4H). In the active responder vehicle males, no differences were seen in peak point compared to baseline (F(4,12)=1.959, p = 0.16; Fig. 4I). However, in the active responder psilocin males, there was a main effect of injection (F(4,16)=6.398, p = 0.002; Fig. 4J) with a significant decrease in peak point at day 2 compared to baseline (p = 0.003), suggesting that psilocin-induced decreases in the amplitude of CeA reactivity were more pronounced in males employing an active vs. passive threat response at baseline.

Changes in CeA reactivity in response to an auditory stimulus following psilocin administration

To test whether the animals developed a conditioned response to the auditory component of the air-puff stimulus, and whether psilocin treatment altered the response to the auditory stimulus alone, two separate auditory-only sessions were conducted immediately following baseline recordings and again at the 28-day recording session. These auditory only sessions followed the same parameters as the air-puff with the exceptions being that the ISI was reduced to 3 min, and the air-puff apparatus was facing outside of the box to maintain the same auditory tone without the physical sensation of the air-puff (Fig. 1A).

In vehicle control females (Fig. 5A), there was a significant decrease in peak point reactivity from the initial to the final auditory condition (t(8)=2.806, p = 0.02; Fig. 5B–D). In psilocin females (Fig. 5E), there were no significant differences between the initial and final auditory session in peak point (t(6)=0.5642, p = 0.59; Fig. 5F–H). In the vehicle males (Fig. 6A), there were no differences in peak point (t(9)=0.8811, p = 0.40; Fig. 6B–D) between the initial and final auditory stimulus. Similarly, in the psilocin-treated males (Fig. 6E), there was no difference in peak point from the initial to the final auditory session (t(10)=0.5758, p = 0.57; Fig. 6F–H).

Fig. 5: Changes in CeA reactivity in response to an auditory stimulus following psilocin administration in female subjects.
figure 5

A Female vehicle group: representative mean ΔF/F traces showing an individual at initial auditory compared to final auditory. Blue line = air-puff onset, Gray lines = individual traces, Green line = mean trace, Std standard deviation. B Female vehicle group: peak point of binned mean ΔF/F traces compared between initial and final auditory recordings. Data points represent each individual subject’s peak ΔF/F value +/− S.E.M. C Female vehicle group: air-puff trace plots of changes in CeA fluorescence following exposure to a 500 ms air-puff at 85 psi. Data points represent group averages within 500 ms binned window +/− S.E.M. D Female vehicle group: Heatmaps comparing initial to final auditory. Each row represents an individual subjects mean trace. E Female psilocin group: representative mean ΔF/F traces showing an individual at initial auditory compared to final auditory. Blue line = air-puff onset, Gray lines = individual traces, Green line = mean trace, Std standard deviation. F Female psilocin group: peak point of binned mean ΔF/F traces compared between initial and final auditory recordings. Data points represent individual subject’s peak ΔF/F value +/− S.E.M. G Female psilocin group: air-puff trace plots of changes in CeA fluorescence following exposure to a 500 ms air-puff at 85 psi. Data points represent group averages within 500 ms binned window +/− S.E.M. H Female psilocin group: Heatmaps comparing initial to final auditory. Each row represents an individual subjects mean trace. In each trace bin plot panel, a significant increase in ΔF/F was determined whenever the lower bound of the 99% CI was >0. These points of statistical significance are shown as colored lines above each ΔF/F curve with colors corresponding to the respective binned traces with a * above the lines. **p < 0.01.

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Fig. 6: Changes in CeA reactivity in response to an auditory stimulus following psilocin administration in male subjects.
figure 6

A Male vehicle group: representative mean ΔF/F traces showing an individual at initial auditory compared to final auditory. Blue line = air-puff onset, Gray lines = individual traces, Green line = mean trace, Std standard deviation. B Male vehicle group: peak point of binned mean ΔF/F traces compared between initial and final auditory recordings. Data points represent each individual subject’s peak ΔF/F value +/− S.E.M. C Male vehicle group: air-puff trace plots of changes in CeA fluorescence following exposure to a 500 ms air-puff at 85 psi. Data points represent group averages within 500 ms binned window + /− S.E.M. D Male vehicle group: Heatmaps comparing initial to final auditory. Each row represents an individual subjects mean trace. E Male psilocin group: representative mean ΔF/F traces showing an individual at initial auditory compared to final auditory. Blue line = air-puff onset, Gray lines = individual traces, Green line = mean trace, Std standard deviation. F Male psilocin group: peak point of binned mean ΔF/F traces compared between initial and final auditory recordings. Data points represent individual subject’s peak ΔF/F value + /− S.E.M. G Male psilocin group: air-puff trace plots of changes in CeA fluorescence following exposure to a 500 ms air-puff at 85 psi. Data points represent group averages within 500 ms binned window + /- S.E.M. H Male psilocin group: Heatmaps comparing initial to final auditory. Each row represents an individual subjects mean trace. In each trace bin plot panel, a significant increase in ΔF/F was determined whenever the lower bound of the 99% CI was >0. These points of statistical significance are shown as colored lines above each ΔF/F curve with colors corresponding to the respective binned traces with a * above the lines.

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Discussion

The present study explored the effects of the psychedelic compound, psilocin, on CeA activity using an immunohistological activity marker and on CeA reactivity to an aversive stimulus using in vivo fiber photometry recordings during acute drug exposure and at 2, 6, and 28 days post drug administration. The study examined multi-level neurobiological and behavioral effects of psilocin by looking at acute drug effects, long-term alterations in activity following drug administration, and by probing for potential sex-specific effects of both injection and time. Psilocin administration produced increases in c-Fos expression in both males and females and sub-region analysis showed that increases in the female CeC were significantly greater in psilocin compared to the vehicle control (Fig. 2B). CeA reactivity in response to the air-puff stimulus following psilocin administration also displayed sex- and time-specific effects. In females, psilocin increased CeA reactivity acutely as compared to vehicle controls (Fig. 2E–H). Interestingly, both vehicle and psilocin groups showed increases in reactivity at specific follow-up timepoints compared to baseline. In the vehicle control females, there were large increases in the duration of CeA response at the 2- and 28-day follow-up (Fig. 3C). In the psilocin treated females, there were small increases at the 2- and 28-day follow-up, with a much larger response seen at the 6-day follow-up (Fig. 3D). Overall, we interpret these data to suggest there is variability in female CeA reactivity as opposed to any effect of the drug. In contrast, the male psilocin group did not show the same increased acute reactivity seen in the females (Fig. 2F, H) and instead displayed decreases in CeA reactivity at 2-day, 6-day, and 28-day follow-up recordings (Fig. 3F, H) that were not seen in vehicle control males (Fig. 3G). These long-term reductions in reactivity seen in males could be due to plasticity dependent changes in innervating circuitry. Future studies will investigate a potential upstream mediator of these observed effects.

In this study, we have replicated previous work demonstrating an increase in basal activity following administration of a 5-HT2AR agonist psychedelic [37]. This could in part be due to local 5-HT2AR excitatory activation in the CeA. However, the CeA contains a high proportion of 5-HT1AR, a presynaptic receptor with inhibitory mechanisms [60]. Another potential explanation lies in more of a circuit/systems level effect of the drug. For instance, the dorsal raphe nucleus is a region within the brainstem that is involved in serotonin synthesis that also has a high density of 5-HT1AR. Previous work has shown that administration of psychedelics produces a local decrease in activity within the DRN that is likely driven by 5-HT1AR [61]. Additionally, it has been shown that there are dense projections of serotonergic neurons from the DRN to the locus coeruleus (LC) [62], and that serotonin release within the LC produces a tonic inhibition of LC neurons [63]. Thus, given previously reported noradrenergic projections from LC to CeA [64], it is possible that disinhibition of the LC through inhibition of the DRN via activation of DRN 5-HT1ARs could result in increased basal activity within the CeA. Future work will look at potential circuit-based mediators of both the observed changes in basal activity and changes in stimulus reactivity within the CeA.

Clinical studies have illustrated that psilocybin produces alterations in amygdala reactivity and connectivity [8, 12, 15, 17, 39,40,41]. Here, we utilized a preclinical approach to examine the actions of psilocin, the psychoactive metabolite of psilocybin, on CeA activity in a rodent model. As most clinical studies report a reduction in amygdala reactivity or connectivity that correlated with therapeutic improvements [8, 15, 17, 39,40,41], we hypothesized that psilocin would produce similar decreases in reactivity in our model. Here we found that a single dose of psilocin produced persistent reductions in CeA reactivity to an aversive stimulus in male but not female rats. These findings lend to recent studies in both clinical [11, 13, 65] and preclinical [66] literature showing persistent reductions in depressive behavior after a single dose of psilocybin. However, given the increases seen in the female group, these data do not perfectly align with clinical findings with samples that include representation from female participants. One potential explanation could be that human fMRI lacks the resolution needed to truly parse apart amygdalar sub-nuclei activation. It is possible that effects seen in the CeA would not match effects seen in the basolateral amygdala (BLA). Additionally, the amygdala has been shown to be involved in both positive and negative valence, and therefore functioning within this region seems to be very dynamic and stimulus-dependent. A surprise air-puff to a rat may be an entirely different experience subjectively than seeing an emotional face, and therefore could result in far different functioning within the amygdala complex. Additional experiments comparing drug effects on whole-brain connectivity/activity will be addressed in future studies. While the specific mechanism behind these therapeutic effects remains unknown, this study provides evidence that changes in CeA activity may play a role in observed therapeutic effects.

Lateralization of amygdala response to fear processing has been noted in both preclinical [67] and clinical literature [68, 69]; however, there are contradictions in these data and therefore no clear, resolute consensus. For instance, one study in human imaging showed that males had greater activation in the right amygdala as compared to females during an emotional face perception fMRI task utilizing angry facial expressions [68]. Another human imaging study employing a similar task showed that amygdala activation differed based on the valence of the stimuli. Additionally, while males had more laterality, both male and female subjects showed greater activation in the left amygdala to fearful faces [69]. This is particularly relevant to the current study given that recordings were taken from a single hemisphere. Initially, hemispheres were chosen based on consistency and quality of signal. Within each group for both males and females, there is representation from both left and right hemispheres. While this does offer some protection from potentially confounding effects of amygdala lateralization, future work will be conducted to determine if there is an effect of amygdala lateralization on psilocin-induced changes in reactivity.

Here, we report sex-specific effects of psilocin on basal activity and stimulus-induced reactivity within the CeA. Changes in basal activity were measured by c-Fos, an immediate early gene shown to be upregulated following neuronal activation [70]. We observed an increase in CeA c-Fos expression in both males and females following administration of psilocin, with region specific increases found in the CeC subregion in females. CeA reactivity was assessed using fiber photometry to measure in vivo stimulus-induced fluctuations in calcium signaling. In the female psilocin group, we observed an acute increase in reactivity to the air-puff stimulus while on the drug that was not seen in the males (Fig. 2H, L). Additionally, we saw increases in reactivity at all of the follow-up recording days, while we saw reductions in CeA reactivity in the psilocin treated males on follow-up recording days (Fig. 3B, D, E, G). A recent study looking at cerebral blood flow and the expression of the serotonin transporter (5-HTT) showed that, in response to an aversive predator odor, there were increases in blood flow and c-Fos expression in the CeA that were related to 5-HTT expression in males but not females [71]. These findings suggest potential sex-specific expression of serotonin receptors within the amygdala. Given the affinity of psilocin for the serotonin system, differential expression of serotonin receptors within the CeA or surrounding nuclei could explain the sex-specific effects seen in this study. Additionally, a previous study showed sex-specific differences in 5-HT2AR expression at baseline, wherein females had lower expression in the orbitofrontal cortex (OFC) than males [72]. Another potential explanation for the differential effects of psilocin between males and females may be due to differences in CeA circuitry. For example, the OFC has been shown, in rodents and non-human primates, to have direct connections to the CeA as well as to the intercalated nuclei, a local GABAergic population within the amygdala complex [73, 74]. Lack of activation of this circuit due to a reduced expression of OFC 5-HT2AR in females could prevent an inhibition of CeA firing, thus causing the increase in reactivity seen while on psilocin in females but not males. Future experiments are needed to examine the effects of psilocin on CeA circuits innervating distinct brain regions.

In the presence of a threatening stimulus, animals will employ either active or passive coping strategies. In this study, the active threat response was characterized by darting, or immediate fleeing from the air-puff stimulus, while the passive threat response was characterized by immobility following the air-puff. Here, we show that females predominantly exhibited an active response, while males were more evenly split between active and passive responses. In male psilocin-treated animals who exhibited an active response at baseline, there was a significant decrease in the amplitude of CeA reactivity following exposure to the air-puff at the 2-day follow-up, with no changes in vehicle control. This decrease in reactivity was not seen in the passive-responder vehicle or psilocin groups. While further interpretation regarding adaptive vs. maladaptive strategies in this context is outside the scope of the current study, these results suggest that there may be differences in CeA reactivity and threat responding strategy that are implicated in the efficacy of psychedelics.

To determine the effects of psilocin on associative learning, we utilized an auditory-only stimulus recording taken after the initial baseline recordings and then again after the 28-day recording. Interestingly, in the vehicle control group, females displayed reductions in CeA response magnitude to the auditory-only stimulus while reactivity in the males did not change. However, In the psilocin group, reductions in CeA reactivity to the auditory stimulus were not observed in males or females (Figs. 5 and 6). Compared to the changes in reactivity seen with the standard air-puff stimulus, the changes in reactivity to the auditory stimulus were contradictory in females and absent in males. These data suggest a more dynamic alteration in CeA reactivity that is stimulus specific. One potential interpretation of the sustained activation in the psilocin females vs. vehicle control is that psilocin may influence associative learning to an aversive stimulus. Several studies have shown that 5-HT2A agonist psychedelics produce improvements in various dimensions of cognition, including associative learning [75,76,77]. Another potential interpretation of these findings is that psilocin created a more robust/meaningful memory of the air-puff. Many of the clinical studies cite participants rating their experience during a psilocybin treatment as one of the most meaningful experiences of their life [13, 78, 79]. Additionally, increases in plasticity could contribute to differences in memory reconsolidation during air-puff administration while on the drug.

Our findings demonstrate that the psychedelic compound, psilocin, produces dynamic, sex-specific changes in activity and reactivity of the CeA, a key nucleus within the amygdala complex. Here we showed that administration of psilocin produces increases in CeA c-Fos expression and that, in females, these increases were primarily driven by increases in expression in the CeC. We have also shown that there are sex-specific changes in CeA reactivity while on psilocin. Specifically, we show that a single administration of psilocin produced acute increases in CeA reactivity in females, but not males. Additionally, we show that persistent reductions in CeA reactivity are seen as early as 2 and as long as 28 days following administration of the drug in males, but not females. Additionally, we show that these reductions in reactivity within the male CeA seem to be driven by those subjects that employed an active coping strategy to the air-puff stimulus at baseline, suggesting that whatever neurobiological mechanism underlying these behaviors may also be contributing to the long-term effects of psilocin. Given that dysregulation of the amygdala is a hallmark in many different psychiatric disorders, including anxiety, MDD, and PTSD [29, 31,32,33,34,35,36] and that psychedelic compounds show promise in treating many of these illnesses [4, 6,7,8,9,10,11], these findings provide important information on subregion-specific alterations in CeA function that may play a part in observed therapeutic effects of psychedelics. One limitation of this work is that no behavioral indices of hallucination (head twitch, wet back shakes, etc.) were collected following administration of the drug. Therefore, no claims can be made regarding the effects of hallucinations on amygdala activity following psychedelic drug exposure. Additionally, without the use of antagonists or known 5-HT2AR-mediated behavioral effects, we cannot claim any sort of receptor specificity in our findings. Though we are unable to implicate involvement of 5-HT2AR in observed effects, it is important to note that while psilocin is an agonist at the 5-HT2AR, it is also a potent agonist at a variety of other serotonin receptors [PDSP Ki Database]. Future work will be required to explore cell-type/receptor-type specificity as well as in probing potential circuit-based mediators contributing to these observed alterations in CeA reactivity. However, the current findings provide important evidence for CeA alterations underlying the potential therapeutic effects of psilocin and psilocybin.