Rett syndrome (RTT) is a severe neurodevelopmental disorder primarily caused by heterozygous loss-of-function mutations in the X-linked gene MECP2 that is a global transcriptional regulator. Mutations in the methyl-CpG binding domain (MBD) of MECP2 disrupt its interaction with methylated DNA. Here, we investigate the effect of a novel MECP2 L124W missense mutation in the MBD of an atypical RTT patient with preserved speech in comparison to severe MECP2 null mutations. L124W protein had a limited ability to disrupt heterochromatic chromocenters due to decreased binding dynamics. We isolated two pairs of isogenic WT and L124W induced pluripotent stem cells. L124W induced excitatory neurons expressed stable protein, exhibited increased input resistance and decreased voltage-gated Na+ and K+ currents, and their neuronal dysmorphology was limited to decreased dendritic complexity. Three isogenic pairs of MECP2 null neurons had the expected more extreme morphological and electrophysiological phenotypes. We examined development and maturation of L124W and MECP2 null excitatory neural network activity using micro-electrode arrays. Relative to isogenic controls, L124W neurons had an increase in synchronous network burst frequency, in contrast to MECP2 null neurons that suffered a significant decrease in synchronous network burst frequency and a transient extension of network burst duration. A biologically motivated computational neural network model shows the observed changes in network dynamics are explained by changes in intrinsic Na+ and K+ currents in individual neurons. Our multilevel results demonstrate that RTT excitatory neurons show a wide spectrum of morphological, electrophysiological and circuitry phenotypes that are dependent on the severity of the MECP2 mutation.
Rett syndrome (RTT) is a rare neurodevelopmental disorder affecting females that is primarily caused by heterozygous loss-of-function mutations in the gene MECP2 . RTT is characterized by early developmental regression leading to microcephaly, loss of language and motor skills, development of hand stereotypies, gait abnormalities, and in some cases autism-like behaviours, autonomic dysfunction and seizures . MECP2 is an X-linked gene that undergoes X chromosome inactivation (XCI) in female cells, resulting in mosaic expression of the wild-type (WT) and mutant MECP2 alleles in RTT individuals that may alter symptom presentation in instances of non-random XCI [3, 4]. Although MECP2 mRNA is ubiquitously expressed, MECP2 protein is found to be most abundant in neurons , where it binds methylated DNA to act as a global transcriptional regulator to repress or activate genes [6, 7]. A large number of RTT patient-associated MECP2 missense mutations map to the Methyl Binding Domain (MBD) and disrupt the interaction with DNA required for proper MECP2 function . A functional consequence of some MBD missense mutations is that they disrupt heterochromatin condensates that are predicted to play a role in disease pathways in RTT .
Human stem cell-derived neuron models of RTT have confirmed many cellular phenotypes observed in Mecp2 mouse models and classic RTT patients [9,10,11,12,13,14,15,16]. In these studies, WT and MECP2 point or null mutation ESCs and iPSCs were differentiated into Neural Progenitor Cells (NPCs) and then into electrically active cortical neurons. They showed that RTT neuron morphology is affected when MECP2 is dysfunctional, exhibiting reduced soma area, dendrite length, branch complexity and number of excitatory synapses. When RTT neuron electrophysiological properties were investigated during patch-clamp experiments [11, 13, 14], they show decreased action potential numbers in response to current pulses, fewer calcium transients and reduced frequency of spontaneous or miniature excitatory post-synaptic currents (mEPSC). These studies consistently demonstrated that classic RTT neurons have decreased size, dendritic branching, excitability and impaired excitatory neurotransmission, together suggesting a neuronal maturation defect.
It has been shown in electrophysiological studies of mouse cortical slices and hippocampal cultures that Mecp2 neurons have decreased mEPSC amplitude or frequency [17, 18]. This implies that decreased excitatory neuronal activity and alteration of activity-dependent gene expression regulated by MECP2 [19, 20] may alter circuit maturation dynamics and contribute to the observed RTT phenotypes. How these changes translate into patterns of activity across networks of neurons in the developing human brain remains poorly documented. Recent approaches using induced excitatory neurons cocultured with astrocytes on micro-electrode arrays (MEAs) now allow evaluation of the development of synchronous network activity and how that circuitry is affected in neurodevelopmental disorders [21,22,23]. It is possible that early activity patterns of synchrony in neuronal networks observed during early brain development are impacted by the identified changes in single neurons, and that the degree of the impact depends on the severity of MECP2 mutations. Simulation models predicting in vivo alterations in circuitry have advanced rapidly and provide an exciting opportunity to predict human circuitry changes in vitro such as variations of synchronic activity patterns during development [24, 25].
In this work, we use a multilevel approach (molecular-cellular-network-modeling) to compare the phenotypes of iPSC-derived excitatory neurons from severe classic MECP2 null mutations to those of an atypical RTT patient with the preserved speech variant. This variant is described as the milder form of RTT, and the patients have diagnostic criteria composed of hand stereotypies, milder reduction in hand skills allowing quite good hand skills, language recovery using single words or complex phrases, normal head circumference, and milder intellectual disabilities . We report a novel missense mutation in the MECP2 MBD at L124W (c.371 T > G) in the atypical patient that has unknown molecular consequences on MECP2 heterochromatin association. We compared atypical L124W to severely affected MECP2 null excitatory neurons and their respective isogenic controls. We investigated protein expression levels, single neuron structure and electrophysiological characteristics (e.g., input resistance, voltage-gated Na+ and K+ currents, action potential firing, and excitatory synaptic function), and neuronal network dynamics using MEA recordings. The results were integrated into a computational network model of spiking neurons. Our results reveal a range of RTT-associated heterochromatin binding, neuron morphology, electrophysiology and circuitry phenotypes depending on MECP2 mutation severity. The computational model simulations further indicate that the observed changes in RTT network dynamics are likely attributable to changes in intrinsic adaptation currents in single neurons.
Limited heterochromatin disruption by MECP2 L124W protein
MECP2 is known to play a role in chromatin architecture . The structure of crystallized human WT MECP2 MBD bound to the BDNF promoter  demonstrated that the location of L124 maps in close 3D proximity to R106 (Fig. 1A). According to RettBASE , a database of patient MECP2 variants, both of these residues are mutated in classic Rett syndrome patients to L124F and R106W, respectively. When these two variants were expressed as GFP fusions in mouse cells they disrupted heterochromatic chromocenters [30,31,32]. To investigate whether the novel L124W MBD missense mutation associated with the preserved speech atypical RTT patient disrupts heterochromatin similarly to L124F and R106W, we assessed its ability to bind and cluster chromocenters relative to WT (instead of null MECP2 variants which cannot be expressed as fusion proteins). In brief, we over-expressed MECP2-GFP fusions in C2C12 mouse myoblast cells, which have low levels of endogenous Mecp2. GFP colocalization with DAPI marked chromocenters was measured by confocal microscopy in cells with similar fluorescence intensities. WT showed distinctly formed chromocenter clusters with high colocalization of GFP and DAPI as expected, while R106W and L124F showed completely disrupted chromocenter clustering and indistinct GFP and DAPI colocalization (Fig. 1B). In contrast, the novel L124W mutation showed limited heterochromatin disruption characterized by unaffected chromocenter number and reduced chromocenter size (Fig. 1B, C). L124W also was more distributed through the nucleus than was WT, showing reduced colocalization of GFP with DAPI as measured by Pearson’s correlation coefficient (PCC) (Fig. 1B, C).
To explore the binding dynamics of L124W protein, fluorescence recovery after photobleaching (FRAP) live-imaging was used to study MECP2 protein mobility . WT and L124W MECP2-GFP fusion proteins were overexpressed in C2C12 cells, and following photobleaching of a single chromocenter focus, MECP2-GFP fusion protein signal recovered more rapidly in the L124W compared with the WT over time (Fig. 1D). L124W had a shorter half-life and larger available unbound mobile fraction to recover the bleached signal that suggests it has decreased binding dynamics with heterochromatin (Fig. 1E). Taken together with the partial impairments in chromocenter clustering, these results point towards a limited effect of L124W relative to L124F and R106W mutations on MECP2 heterochromatin condensates due to decreased binding dynamics.
Generation of MECP2 L124W iPSCs and Ngn2-derived excitatory neurons
As differences in genetic background and modifier genes contribute to RTT phenotype severity [33, 34], disease modeling strategies using isogenic WT cells that are reprogrammed at the same time as mutant cells, or derived from the same iPSC line by gene editing, provide the most precise controls for comparing to affected cells. The use of isogenic controls addresses variability observed across different WT controls especially in the context of network circuitry studies , and variability can be further addressed by comparing isogenic pairs of cells in different wells within the same MEA plate. By taking advantage of XCI in female somatic cells, we and others have generated isogenic pairs of RTT stem cell lines from a single reprogramming experiment. As described below, we first generated isogenic human iPSC bearing the L124W missense mutation. As controls, we chose MECP2 null cells including isogenic lines derived from a d3-4 patient  and a homozygous MECP2 null line derived by gene editing of the human ESC line WIBR3  that cannot reactivate a WT allele (Fig. 1F). We also generated a third MECP2 null isogenic pair using gene editing of the PGPC14 healthy control iPSC line because whole genome sequencing showed it has no known genomic variants associated with neurological disorders .
We generated iPSCs from the atypical RTT patient (CLT) harbouring a heterozygous MECP2 c.371 T > G variant resulting in the L124W amino acid change. iPSCs were reprogrammed from patient fibroblasts using Sendai virus, and STR analysis verified the lines were derived from the fibroblasts. The lines were first assessed for XCI status using the androgen receptor (AR) endonuclease digestion assay to identify isogenic lines fully skewed to either the WT or L124W allele on the active X chromosome (Xa) with a male line as a control for full digestion of an unmethylated Xa (Fig. 1G). The terminology used for these CLT isogenic cell lines is CLT-WT and CLT-L124W. Two lines of Xa CLT-WT (C4 and C17) and two lines of Xa CLT-L124W (C1 and C2) were obtained with normal 46XX karyotype (Fig. S1A). The iPSC lines demonstrated pluripotency by positive staining for OCT4, NANOG, SSEA-4 and TRA-1-60 (Fig. S1B), spontaneous differentiation into the 3-germ layers (Fig. S1C) and pluripotent cell expression profiles determined by RNA-seq and Pluritest analysis  (Fig. S1D). After this continued passage for pluripotency characterization, restricted mRNA expression of the Xa allele was again confirmed by cDNA sequencing across the L124W variant (Fig. 1H). In this way, the inactive X chromosome (Xi) was shown to not be subject to erosion  at two timepoints early in iPSC isolation and after full characterization just prior to differentiation of the lines into neurons.
To examine L124W protein levels in excitatory cortical neurons we used a rapid induced Neurogenin-2 (Ngn2) overexpression protocol [39, 40] (Fig. S1E). In brief, WT and RTT iPSCs/ESCs were transduced with the Tet-inducible Ngn2-puro lentivirus vectors, overexpression was induced with doxycycline for 8 days and selected with puromycin in the presence of GDNF and BDNF, and dividing cells were eliminated with Ara-C prior to excitatory neuron re-plating and maturation. As expected, MECP2 capillary-based protein detection of 6 week old Ngn2 neuron extracts demonstrated absence of MECP2 protein in d3-4 and WIBR3 null lines, but the levels of MECP2 protein were similar in isogenic CLT-WT and CLT-L124W lines (Fig. 1I). We conclude that L124W mutant protein is present and produced at unchanged levels in CLT-L124W cells.
While we proceeded to evaluate the CLT, d3-4 and WIBR3 neurons for neuronal activity as described in the next section, we used CRISPR/Cas9 gene editing to isolate a novel MECP2 null line. We introduced indels into a previously characterized healthy control female PGPC14 iPSC line from the Personal Genome Project Canada . PGPC14 iPSCs were co-transfected with Cas9 and a gRNA specific for exon 3 of MECP2. Selected clones were screened for normal karyotype (Fig. S1F) and found to be positive for hallmark pluripotency markers (Fig. S1G). cDNA sequencing identified a 1 bp insertion (c.217_218insC) into MECP2 exon 3 on the Xa (Fig. S1H) of the PGPC14 null line, resulting in a frameshift and premature truncation of the protein (p.Val74Cysfs*16) early in the MBD (Fig. 1F). Generation of Ngn2 neurons and protein detection by immunocytochemistry (Fig. S1I), capillary (Fig. 1J) and conventional western blot (Fig. S1J) demonstrated that PGPC14 indel neurons were MECP2 null, compared with d3-4 null samples generated from NPC derived neurons, and neither null line reactivated the Xi to produce WT protein. The PGPC14 parental cells exhibit robust differentiation into many cell types, in contrast to the d3-4 lines, and were employed for subsequent neuron morphology and circuitry experiments.
RTT Ngn2 neurons exhibit a range of alterations in intrinsic membrane properties and excitatory synaptic function
To validate Ngn2-derived excitatory neurons as an appropriate cellular model of RTT, we examined their electrophysiological characteristics and first determined whether there were detectable differences in the known MECP2 null associated phenotypes. We made whole-cell patch-clamp recordings to investigate the intrinsic membrane properties of 4-5 week-old Ngn2 neurons from isogenic d3-4 and WIBR3 MECP2 null lines co-cultured with mouse astrocytes. We looked for alterations in electrophysiological characteristics as previously reported in mouse iPSC-derived neurons and human RTT neurons conventionally differentiated via an NPC stage [11, 41]. Indeed, relative to isogenic controls MECP2 null neurons exhibited depolarized resting membrane potential, higher input resistance and decreased cell capacitance (Fig. 2A–C), consistent with the previous findings on RTT iPSC-derived neurons. We next assessed whether MECP2 null mutant neurons had alterations in active membrane properties, as previously reported in RTT neurons [11, 41]. Voltage-gated Na+ and K+ currents, under voltage-clamp condition, were evoked by depolarizing voltage steps from a standardized holding potential of −70 mV. We found that both of Na+ and K+ currents were diminished in d3-4 and WIBR3 null neurons, compared with their respective isogenic WT (Fig. 2D). Given these alterations in the electrophysiological characteristics, MECP2 null neurons were anticipated to have dysfunction in action potential firing. We therefore performed whole-cell current-clamp recordings to investigate the characteristics of action potentials, which were elicited by recording in current-clamp mode and injecting a series of current steps from −5 pA to 100 pA in both WT and MECP2 null neurons (Figs. 2E & S2A–E). The majority of action potential parameters were not significantly different across the groups (Fig. S2A–E), dysfunction in the generation of action potentials was observed in MECP2 null neurons as compared with the respective WT controls (Fig. 2E). Together, these findings indicate that loss of MECP2 causes alterations in electrophysiological characteristics and deficiency in action potential firing, which may lead to a further influence on the functions of the neuronal network. We next examined synaptic transmission by recording mEPSCs in 6-week-old Ngn2 WT and MECP2 null neurons from d3-4 and WIBR3. We observed reduced AMPAR-mEPSC amplitude and frequency in the MECP2 null neurons (Fig. 2F), consistent with previous reports of hypo-activity in RTT iPSC-derived cortical neurons [11, 41]. Taken together, these data indicate that MECP2 null Ngn2 neurons recapitulated known RTT-associated excitatory synaptic phenotypes.
Based on the limited disruption of heterochromatin condensates by L124W MECP2 protein, we predicted that CLT L124W Ngn2 neurons would have a milder cellular phenotype in comparison to their isogenic WT neurons. In CLT L124W neurons the input resistance was greater than in the CLT WT neurons. However, neither resting membrane potential nor cell capacitance in CLT L124W neurons were different from those in CLT WT neurons (Fig. 2A–C). Voltage-gated Na+ and K+ currents in CLT L124W neurons were smaller than those currents in CLT WT neurons (Fig. 2D). However, CLT L124W did not display deficiency in firing action potentials (Fig. 2E). Next, when examining excitatory neurotransmission in CLT WT neurons and CLT L124W Ngn2 neurons, neither AMPAR-mEPSC amplitude nor frequency in CLT L124W neurons were different from CLT WT neurons (Fig. 2F). Therefore, recordings from CLT WT and CLT L124W Ngn2 neurons show increased input resistance and impaired voltage-gated Na+ and K+ currents in the mutant neurons. Greater magnitude increases in input resistance and impairment of Na+ and K+ currents were also observed in MECP2 null neurons. In addition to this core subset of phenotypes shared with CLT L124W neurons, MECP2 null neurons showed differences, in comparison with their isogenic controls, in resting membrane potentials, cell capacitance, action potential firing, and excitatory synaptic function.
Altered neuronal size, complexity and excitatory synapse density in RTT Ngn2 neurons
Reduced size and complexity have been identified as prominent morphometric features of classic RTT using human NPC-derived cortical neurons [10, 13, 14]. To assess morphometry in Ngn2 neurons, 6 week old neuron co-cultures with mouse astrocytes were sparsely labelled with GFP in 3–5 independent experiments. Fixed cells were stained for DAPI, GFP and MAP2, and images captured on a confocal microscope reveal similar cell densities for all genotypes (Fig. 3A). Soma area and dendrite tracing was performed by two observers blinded to genotype. As anticipated, MECP2 null (d3-4, WIBR3 and PGPC14) neurons had decreased average soma area and total dendrite length, recapitulating the known classic RTT-associated phenotypes that are implicated in microcephaly (Fig. 3B, C). In contrast, the CLT L124W missense neurons exhibited no changes in soma area or dendrite length (Fig. 3B, C), consistent with the normal head circumference of preserved speech variant atypical RTT and with our finding that capacitance is not changed.
However, Sholl analysis of the CLT L124W neurons revealed decreased branching complexity relative to WT, similar to the reduced branching complexity in MECP2 null neurons relative to WT (Figs. 3D & S3A). To investigate changes in synapses, excitatory synapse density was quantified based on co-localization of pre-synaptic SYN1 and post-synaptic HOMER1 puncta along dendritic MAP2 signal. The d3-4, WIBR3 and PGPC14 MECP2 null neurons showed reduced SYN1/HOMER1 density compared to its respective isogenic control. On the other hand, CLT L124W neurons did not show significant changes relative to WT (Fig. 3E). Taken together, CLT L124W neurons show only the core subset of dendrite complexity changes shared with MECP2 null neurons, consistent with more limited mutation-dependent phenotypes found in L124W protein heterochromatin association and excitatory neuron activity.
Aberrant network circuitry in RTT Ngn2 neuronal cultures
During development, individual neurons connect to one another forming neuronal networks that produce distinctive patterns of synchronized activity. Such patterns play a critical role in shaping the network structure and function in the developing brain . Upon observing alterations in functional and morphometric properties on the level of individual neurons, we determined if integrated neural circuit activity would be perturbed in RTT cultures. Isogenic WT and RTT Ngn2 neurons (WIBR3, PGPC14 and CLT-L124W) were co-cultured with mouse astrocytes on 12-well MEA plates with 64 channels in an 8 × 8 grid per well (Axion Biosystems) (Fig. S4A). Every plate was derived from an independent differentiation from Ngn2-inducible iPSCs/ESCs and contained 3–6 wells of each isogenic genotype as technical replicates. Time zero was the day when neurons and astrocytes were plated together in co-culture, and five minutes of extracellular recordings were collected each week from week 3 through week 7.
We observed the emergence over time of high frequency neural activity in multiple electrodes similar to those observed in previous experiments . Raster plots of action potentials detected using Axion software show synchronous bursts across multiple electrodes for the WIBR3, PGPC14 and CLT isogenic pairs (Fig. 4A). As anticipated, we detected variability of firing regimes across the different WT lines (Fig. 4A) despite equivalent cell densities on the electrodes (Fig. S4A). This is consistent with a published guideline that reports variability across 10 different control lines and stresses the importance of using isogenic controls for assessing disease-associated phenotypic changes in human stem cell derived neural networks . The number of electrodes with active signals increased over the time course of the recordings likely as a result of development of new connections within the network, but there was plate-to-plate variability in numbers of active electrodes (Fig. S4B). MEA plates were analyzed if at least one line on them had wells that reached a cut-off of at least 32 out of 64 electrodes active over the time course to reduce variability. With this cut-off, we could include plates that developed strong network activity and exclude ones with poor cell adherence. Additionally, at the final time point neurons were treated with the AMPAR antagonist CNXQ to show that spiking activity was abolished (Fig. S4C). This supported that synaptic transmission was responsible for network activity patterns.
Based on the mild electrophysiological and morphological differences in CLT L124W neurons, we predicted a similarly small change in variables that measure network activity. To account for differences in maturation dynamics between plates  we normalized the fold-change of RTT values to the respective WT isogenic values within a plate. We examined 6 biological replicate plates of CLT isogenic neurons, each with 3 wells of the four CLT lines. Cumulatively, the CLT L124W Ngn2 neurons did not show significant differences in weighted mean firing rate (defined as the mean firing rate across active electrodes normalized to the number of active electrodes per well) relative to WT (Fig. 4B, right). At late timepoints, increased network burst frequency and decreased network burst duration were observed (Fig. 4C, D, right). Absolute values for the above network metrics are shown in Fig. S4B–D, and no other differences were observed in interburst interval, synchrony or other metrics.
To detect circuitry defects in MECP2 null neurons, we then examined 6 biological replicate MEA plates of WIBR3 and 4 biological replicate MEA plates of PGPC14 isogenic pairs, each with 6 wells of isogenic WT and nulls. The RTT values are shown as relative log fold change compared to the WT baseline (normalized WT level indicated by dashed horizontal line). The weighted mean firing rate did not have a consistent change in MECP2 null neurons relative to WT (Figs. 4B and S4D). However, the MECP2 null network bursts showed a consistent decrease in frequency relative to WT beginning at the 4 week time-point (Figs. 4C and S4E). These decreased network burst frequencies were followed by longer network burst durations that transiently emerged at weeks 5–6 in WIBR3 and PGPC14 isogenic lines (Figs. 4D & S4F). The WIBR3 null isogenic lines had larger fold changes in network bursting properties compared to the PGPC14 null isogenic lines. In contrast, CLT L124W had only late time points with increased network burst frequency or decreased network burst duration that were distinct relative to those shown by MECP2 null networks.
Modeling changes in peak bursting frequency in RTT Ngn2 neural networks
Variations in the frequency of network bursts capture differences between RTT and WT, particularly for the MECP2 null phenotypes showing more pronounced changes in cellular and network profiles. In order to better characterize such changes in the frequency domain and search for more subtle differences between the different network phenotypes we extracted raw spiking data and performed power spectral density (PSD) estimations on spike trains from each electrode within a well. The PSD calculated directly on recorded spike trains in all 64 channels exhibits clear peaks shown in heatmaps of the frequency of these synchronous network bursts (Fig. 5A). When the distributions of peak bursting frequencies were aggregated from weeks 2–7 (Figs. 5B and S5A) or normalized to their respective isogenic control (Fig. 5C), the WIBR3 and PGPC14 nulls were reduced in cumulative median peak bursting frequency across all timepoints. The peak bursting frequency of CLT L124W was slightly increased. Our PSD estimates consistently distinguished MECP2 null from WT Ngn2 neural networks, and captured the difference between the direction of peak burst frequency change in the CLT L124W compared to MECP2 null neurons.
Changes in burst frequency can have at least two different origins, one is changes in the intrinsic properties of individual neurons, and the second is changes in network synaptic connectivity. First, to test whether the observed differences in peak burst frequency in RTT networks were based on changes to altered intrinsic properties (for example voltage-gated Na+ and K+ currents, capacitance or resting membrane potentials) we developed a computational model of excitatory spiking neurons connected into a network that approximates the iPSC-derived cultures. A network of 1000 excitatory adaptive integrate-and-fire (AdIF) neurons [43, 44] was simulated using parameters derived from the patch-clamp recordings. We modelled random intra-network connections, connection probability of 0.05 (resulting in an average of 50 synapses per cell), and random Poisson external input (100 synapses spiking at 20 Hz). This spiking model displayed robust network bursts, the features of which are related to single-neuron properties in the network. We then analyzed simulated spike trains in the same way as the experimental data. Overlay of the peak frequency derived from the top fit AdIF simulations (red line in Fig. 5A) demonstrated that this spike adaptation-driven model recapitulated the reduced frequency observed in the WIBR3 and PGPC14 null neuron recordings. Strikingly, the AdIF simulations also captured the effect in the opposite direction for the CLT L124W neurons where the network peak frequency was increased. This model demonstrates that changes in Na+ and K+ currents, capacitance or resting membrane potential, or combinations of them, are sufficient to account for the network peak burst frequency differences.
Next, we addressed whether systematic differences in network/synaptic connectivity between RTT and WT networks could cause the observed differences in burst frequency. To this end we studied the WIBR3 network model over a range of subthreshold adaptation values (4 to 10 nS) and connection probabilities (0.05 to 0.35). Over this range, network burst frequency increases with the decreased connection probability typically observed in RTT neuron networks , while the value of subthreshold adaptation has a smaller effect (Fig. S5B). The effect of connection probability on burst frequency is opposite to that observed in the experimental data, indicating that changes in synaptic connection probability do not explain our observations. This control simulation is consistent with intrinsic adaptation currents driving the observed changes in burst frequency.
Finally, to determine the contributions of Na+ and K+ currents relative to membrane capacitance and resting potential properties in driving peak burst frequency patterns in RTT, we performed drop out simulations by replacing the WIBR3 null values with their isogenic WT values. As shown in Fig. 5D, simulation with the null values gives a low frequency of bursts over the representative 30 s duration. When the null Na+ and K+ channel values, represented by the adaptation currents, are dropped out and replaced by the WT values, the simulation predicts that burst frequency is rescued. In contrast, when the null membrane values (capacitance and resting membrane potential) are dropped out and replaced by the WT values, there is no substantial change to the frequency. Reciprocal simulations where the WT values are dropped out and replaced by the null values also predict that it is only the null Na+ and K+ channel currents that drive the slow bursting frequency. The simulation model over 300 s durations (Fig. 5E) agrees with the experimental circuitry findings for both the MECP2 null and CLT L124W neurons, and indicates that the MECP2 null burst frequencies are driven by experimentally validated changes in Na+ and K+ currents.
In this study, we defined a wide phenotypic spectrum of MECP2 mutation-dependent changes in heterochromatin binding, neuron morphology, electrophysiological properties and network connectivity by modeling atypical and classic RTT neurons in vitro. We investigated the effects of a novel MECP2 L124W MBD atypical variant in comparison with three classic MECP2 nulls in isogenic induced excitatory neurons. Our findings uncover core functional and morphological phenotypes present in both types, and a broader set of additional impairments exhibited by null neurons (summarized in Table 1). The core phenotypes present in atypical RTT included increased input resistance, impaired voltage-gated Na+ and K+ currents, and reduced dendritic complexity. MECP2 null neurons consistently displayed the same core phenotypes as well as depolarized resting membrane potential, reduced cell capacitance accompanied by decreased soma area, dendrite length and excitatory synapse density, and had disruptions in excitatory synaptic transmission. Both the L124W and null neurons exhibited a shared core phenotype in neuronal network burst frequency and duration patterns but these changed in opposite directions. Simulation modeling using parameters derived from the patch-clamp recordings replicated the increased network burst frequency in L124W and the decreased frequency in null neurons. Drop out simulations on the null neurons predicted that network burst frequency changes were driven by Na+ and K+ channel currents. Thus, the MECP2 null phenotypic profile encompassed known RTT-associated changes that validated our induced neuron model and revealed novel insights into the development of excitatory synaptic activity and network circuitry.
L124W molecular, cellular and network core phenotypes
The atypical RTT-associated phenotypic profile of L124W MECP2 protein was supported by evidence of partially disrupted chromocenter binding in mouse cells and stable unchanged protein level in induced neurons. The chromocenter assay was recently used to demonstrate that MECP2 is a component of heterochromatin condensates that are predicted to play a role in disease pathways in RTT, and that MBD missense mutations often compromise this activity . This mechanism likely alters the set of genes repressed by L124W MECP2 in neurons. One potential experimental artefact we excluded was XCI erosion in the CLT L124W iPSCs. Since expression of the Xi was not reactivated, no WT MECP2 was produced in neurons to rescue L124W phenotypes. Overall, the L124W variant represents a subtype of MECP2 MBD mutation that retains partial function in contrast to the more disrupted heterochromatin found in previous studies of L124F, R106W and other MBD missense variants .
We found that CLT L124W neurons showed core phenotype changes in dendrite complexity and intrinsic properties similar to the WT. Importantly, CLT L124W neurons showed increased input resistance. As a consequence of higher input resistance a given depolarizing current will produce a larger change in membrane potential. Thus, increased input resistance explains our finding that the amount of depolarizing current required to evoke an action potential was lower in all mutants as compared with their corresponding controls (Fig. 2E). From this result we conclude that RTT neurons have increased excitability because they have a greater chance to trigger an action potential with a given depolarizing current. A recent study on R106W missense iPSC derived neurons also found a graded response in some morphological features and electrophysiological properties compared to MECP2 knockdown neurons, and did not detect changes in synaptic function that were evident in knockdown neurons . In our case we also examined Na+ and K+ currents in CLT L124W and MECP2 null neurons, and found these currents are consistently decreased relative to those in WT. As we found limitation of sustained firing of action potentials in response to prolonged current injection in mutant neurons, as compared to WT, we conclude that the decrease in Na+ currents predominates over the decrease in K+ currents (Fig. 2E). In addition, our network circuitry studies using MEA revealed a core phenotype that network burst frequency and duration are changed, and the opposite direction of this change in CLT L124W and MECP2 null neurons was successfully modelled computationally.
A potential role for modifier genes that mitigate the cellular and network phenotypes in the atypical RTT neurons could be examined in future by whole genome sequencing for known variants, and by generating a null mutation in the active MECP2 allele of the CLT L124W iPSC lines to test if their phenotype is milder than the consistent phenotype observed in the three MECP2 null mutants. We also recognize the possibility that L124W may have functional effects on other cell types. For example, RTT astrocytes have been shown to exert non-cell autonomous effects on WT and mutant neurons [47, 48]. As inhibition was absent in our excitatory neuron model, co-culturing CLT L124W astrocytes with glutamatergic and GABAergic neurons in monolayers or organoids could explore the potential interplay of these signals in single neurons and network activity [49,50,51].
MECP2 null full phenotype and modeling of RTT networks
The MECP2 null full phenotype included smaller soma size and dendrite length, reduced synapse numbers, and reduced AMPAR-mEPSC amplitude and frequency. Importantly, we observed robust network bursting phenotypes in MECP2 null neurons. The multi-unit spiking pattern changes revealed that network-wide events occurred less frequently and had extended burst trains in MECP2 nulls compared to WT. In molecular terms, when there is complete loss of MECP2 protein all interactions of cofactors with any MECP2 domain would be abrogated. The outcome would be expected to impact transcription of every gene that is directly regulated by MECP2 binding, and there may be additional impacts on miRNA processing that would affect mRNA stability, splicing to generate alternative isoforms, and strong reductions in protein translation. These latter pathways, in combination with the more severe effects on direct transcription targets, could have complex outcomes in circuits that differ in direction from those in L124W neurons.
The MECP2 null circuitry phenotype shows similarity to the reduced rate and increased duration of network bursts observed in Kleefstra syndrome induced excitatory neural cultures cocultured with rodent astrocytes, where these changes were found to be mediated by upregulation of N-methyl-D-aspartate receptor subunit GRIN1 expression . Highly perturbed RTT-associated oscillatory network activity phenotype in local field potential MEA recordings of isogenic WT and MECP2 knockout iPSC-derived cortical organoids showed complete absence of network events .
A possible explanation for changes in network dynamics are changes in the intrinsic properties of neurons that may impact their ability to generate bursting patterns. Our spiking network model examined this issue. Model simulations indicate that the changes in RTT null networks bursting frequency may be mediated by neuronal adaptation currents such as inactivation of depolarizing Na+ currents, and activity dependent (i.e. calcium dependent) activation of slow hyperpolarizing K+ channels [43, 52], which may be downstream targets of MECP2 [15, 53]. This is supported by the decrease in K+ currents seen in RTT neurons (Fig. 2). We have also previously found that MECP2 null neurons derived from NPCs have altered protein levels of K+ channels and AMPA receptors . Finally, the computational drop-out simulations support the hypothesis that changes in intrinsic Na+ and K+ currents are the driving variable of the observed changes in burst frequency. Because these currents are known to be critical in shaping neuronal excitability, gain, and responses to sensory input, these changes could play important roles in cortical function [44, 55].
In summary, we used a multilevel approach to identify a range of RTT-associated phenotypes relating to form and function in isogenic human stem cell-derived excitatory neurons harbouring a novel L124W missense or MECP2 null mutations. We demonstrate a mild heterochromatin association phenotype in L124W MECP2 that is mutated in an atypical RTT patient with the preserved speech variant. We define a core set of cellular and network phenotypes in atypical RTT neurons, and identify additional synaptic and morphological phenotypes present in null neurons. Network burst frequency and duration changes were observed but in opposite directions for atypical and null neurons. Our simulations demonstrate the utility of spiking models for analyzing changes in stem cell-derived RTT neuron networks and these simulations can be applied to the study of other neuropsychiatric disease states. The striking network burst frequency changes revealed in MECP2 null neurons provide a potentially screenable phenotype for testing candidate compounds to rescue the RTT-associated changes in this in vitro assay to moderate disease. Full rescue might require correction of protein translation regulation and thus of the Na+ and K+ channel current deficits found in the core phenotypes.
Protein model, chromocenter and FRAP assays
Human MECP2-MBD crystallographic structure was illustrated using PyMol Molecular Graphic System (PDB accession code: 3C2I), showing MBD bound at methylated double stranded DNA sequence of BDNF promoter, with point mutation location indicated. All imaging and image analysis for chromocenters were performed as previously described [32, 56] using the Fiji Particle Analysis plugin. FRAP Time-lapse imaging was carried out as a series of confocal time lapse images of frames (512 × 512 pixels) imaged at 488 nm laser excitation with 0.05 transmission were used to record GFP-tagged protein post bleach recovery. FRAP assay was recorded with a minimum of nine pre-bleach frames, one frame after 1000 µs bleaching with 405 nm laser line at 100% transmission, and 90 post-bleach frames were recorded at equal time intervals.
Ethics and iPSC reprogramming
iPSCs were reprogrammed under approval of the Canadian Institutes of Health Research Stem Cell Oversight Committee and the Research Ethics Board of The Hospital for Sick Children (REB #1000050639) and the University of California San Diego (IRB #14123ZF). Existing iPSC lines are listed in Table S1.
CLT iPSC lines were generated from fibroblasts after informed consent was obtained. Fibroblasts were collected from a small incision in the skin after a skin biopsy and tissue was washed in PBS 1x with 4% penicillin/streptomycin (Gibco). Later, the tissue was broken into small pieces using needles and plated in culture media (DMEM/F12, 15% FBS, 1% MEM NEAA, 1% L-glutamine and 1% penicillin/streptomycin). After 1 month, fibroblasts migrated out from the tissue. After reaching high confluency, cells were expanded or frozen as stock [57, 58]. Fibroblasts were reprogrammed using Sendai virus (Invitrogen) in feeder-free conditions on Matrigel (Corning) with mTeSR1 (STEMCELL Technologies). After colonies started to grow, colonies were picked manually and expanded on feeder-free Matrigel-coated dishes and frozen with CryoStore (STEMCELL Technologies). Mycoplasma contamination was routinely checked.
iPSC/ESC culturing and maintenance
Human iPSC/ESCs (Table S1) were maintained on dishes coated with Matrigel in mTeSR1 or StemMACS iPS-Brew XF (Miltenyi Biotec) media with 1% penicillin/streptomycin. iPSCs were passaged weekly with ReLeSR (STEMCELL Technologies) with daily media changes except for the day following passaging. Accutase (Innovative Cell Technologies) and media with 10 μM Rho-associated kinase (ROCK) inhibitor (Y-27632, STEMCELL Technologies) was used for any single-cell dissociation. Routine mycoplasma testing was performed.
iPSC karyotyping and standard G-banding chromosome analysis (400-banding resolution) was performed by The Centre for Applied Genomics (TCAG) at The Hospital for Sick Children.
iPSC colonies were fixed and stained with pluripotency markers OCT4, NANOG, SSEA4 and TRA-1-60. Following embryoid body formation and spontaneous 3-germ layer differentiation for 16 days, resulting cells were fixed and stained for α-SMA, AFP and β-III-tubulin. To assess gene expression profile of iPSCs, RNA was extracted from 1 confluent well of a 6-well plate using RNA PureLink RNA mini kit (Life Technologies) as per manufacturer’s protocol. Samples were sent to TCAG at the Hospital for Sick Children for Bioanalyzer assessment and sequenced as paired end 2 × 125 bases on an Illumina HiSeq 2500 at a depth of 15 M reads. Resulting FASTQ files were uploaded to www.pluritest.org for analysis . Files can be found with GEO accession GSE148435.
Androgen receptor (AR) assay was performed as previously described . In brief, 400 ng of genomic DNA from iPSCs/ESCs was digested by CpG methylation-sensitive restriction enzymes HpaII and HhaI for 6 h. 2 μL of digestion was amplified using Platinum Taq DNA Polymerase High Fidelity (Invitrogen) with AR gene primers with a FAM label (Table S2). Male samples were used as a control to confirm complete digestion. Electrophoresis was performed by TCAG at The Hospital for Sick Children. Traces were analyzed using PeakScanner (Thermo Fisher Scientific). To identify which MECP2 allele was expressed on the active X chromosome, RNA was isolated from iPSCs and reverse transcribed into cDNA using SuperScript III (Invitrogen). Primers flanking the variant region (Table S2) were used for amplification with Platinum Taq DNA Polymerase High Fidelity or Q5 High Fidelity DNA Polymerase (New England BioLabs), followed by cloning as per the TOPO TA Cloning Kit (Invitrogen) or Zero Blunt TOPO Cloning Kit (Invitrogen) in OneShot TOP10 (Thermo Fisher Scientific) or Max Efficiency DH5α (Invitrogen) competent E. coli strains. 5–10 bacterial colonies per iPSC cell line tested were picked and grown in LB Medium (MP Biomedicals) for DNA extraction with Quick Plasmid MiniPrep Kit (Invitrogen). Samples were Sanger sequenced at TCAG and aligned to WT MECP2 template using benchling.com.
CRISPR/Cas9 gene editing
The MECP2 indel mutation was generated using a previously described gene editing strategy . Briefly, pSpCas9(BB)−2A-Puro (PX459) V2.0 (Addgene #62988) was cloned with an oligonucleotide designed to target MECP2 exon 3 using benchling.com CRISPR prediction tool (described in Table S2). Indels were introduced by transfecting 8 × 105 cells with 1.5 μg plasmid in 100 μl scale using the Neon Transfection System (ThermoFisher) with one pulse at 1500 millivolts for 30 milliseconds (~40–70% transfection efficiency). Transfected cells were plated in 2 wells of a 6-well plate with mTeSR1 media supplemented with CloneR (STEMCELL Technologies) to enhance cell survival. From D2-5, puromycin (0.5 ug/ml) was added daily to mTeSR1 media changes and surviving single colonies were grown to D12-18 before transferring to 24-well plates. Isolated clones were passaged and gDNA was harvested with a Quick DNA miniprep kit (Zymo) and PCR products were sent for Sanger sequencing by TCAG at the Hospital for Sick Children.
Lentivirus generation and transduction
FUW-TetO-Ngn2-P2A-EGFP-T2A-puromycin and FUW-rtTA plasmids for excitatory cortical neuron differentiations were kindly gifted by T. Sudhof  and packaged as per a previously described protocol . Lentiviruses were produced in HEK293T cells using a third generation packaging system (pMDG.2, pRSV-Rev and pMDLg/pRRE). Viral particles in the supernatant were collected 48 h post-transfection, filtered and concentrated by 91,000 × g centrifugation for 2 h at 4 °C. For iPSC transductions, accutase (Innovative Cell Technologies) was used to obtain 5 × 105 cells single-cells per well of a Matrigel-coated 6-well plate that were incubated with concentrated virus in mTeSR1 with 10 μM ROCK inhibitor and 8 μg/ml polybrene (Sigma) for 8 h. Media was changed to mTeSR1 and transduced iPSCs were maintained and expanded to acquire frozen stocks.
Ngn2 neuronal direct conversion
Excitatory cortical neurons were generated as previously described [36, 39]. Briefly, on Day 0 iPSCs were made into single-cells by accutase and plated at 3 × 105–1 × 106 cells per well on Matrigel-coated 6-well plates with StemMACS iPS-Brew XF media supplemented with 10 μM ROCK inhibitor. All media mentioned for Day 1–8 contained 1x penicillin/streptomycin (Gibco), 1 μg/mL laminin (Sigma), 2 μg/mL doxycycline hyclate (Sigma), 10 ng/ml BDNF (Peprotech) and 10 ng/mL GDNF (Peprotech). On Day 1, media was changed to CM1 consisting of DMEM/F12 (Gibco), 1x N2 (Gibco) and 1x NEAA (Gibco), supplemented with 10 μM ROCK inhibitor. On Day 2, media was changed to CM1 supplemented with 2–5 μg/mL puromycin (Sigma). On Day 3, media was changed to CM2 consisting of Neurobasal (Gibco), 1x B27 (Gibco) and 1x Glutamax (Gibco), supplemented with 2–5 μg/mL puromycin. On Day 4–5, media was changed to CM2. On Day 6, media was changed to CM2 supplemented with 10 μM Ara-C (Sigma). On Day 8, doxycycline induction was withdrawn and neurons were re-seeded onto coverslips or wells coated with 0.1 mg/ml poly-L-ornithine (PLO) (Sigma) and 40 μg/ml laminin (Roche or Sigma) in CM2 with BDNF and GDNF and co-cultured with P1 mouse astrocytes with media changes every 3–4 days unless otherwise noted for downstream assays.
Protein was extracted from neurons (not grown with mouse astrocytes) washed in ice-cold PBS using radioimmune precipitation assay (RIPA) buffer (25 mM Tris-HCl, pH 7.6, 150 mM NaCl, 1% Nonidet P-40, 1% sodium deoxycholate and 0.1% SDS). Equivalent protein masses were loaded into capillaries for detection using the 12–−230 kDa Separation Kit for the Wes (ProteinSimple). Multiplexed probing of MECP2 and β-actin by chemiluminescence was quantified using Compass for SW software (ProteinSimple).
Electrophysiological recordings were made as reported previously [11, 41]. Briefly, whole-cell patch-clamp recordings were performed with an Axopatch 1-D amplifier (Molecular Devices, USA) operated with Clampex 9.2 software and a DigiData 1200 series interface (Molecular Devices, USA), and the currents were sampled at 10 kHz and filtered at 2 kHz. The recordings were analyzed off-line using Clampfit 9.2 software (Molecular Devices, USA). All electrophysiological experiments and data analysis were conducted with Ngn2 neuron samples blinded to the investigator. To examine intrinsic membrane properties, the recordings were performed in a 35-mm tissue culture dish filled with an extracellular solution containing (in mM): 140 NaCl, 5.4 KCl, 1 MgCl2, 15 HEPES, 2 CaCl2, 10 glucose, and the solution was adjusted to pH 7.35 with NaOH. Patch-clamp pipettes (5 to 8 MΩ) were pulled from capillary glass (1.5 mm diameter; World Precision Instruments, USA) using a P-87 pipette puller (Sutter Instrument Co., USA), and filled with an intracellular solution compose of (in mM): 144 K+-gluconate, 10 KCl, 2 Mg-ATP, 2 EGTA, 10 HEPES. The solution was adjusted to pH 7.2 with KOH. Voltage-gated ion currents, under the whole-cell voltage-clamp condition, were elicited at the holding membrane potential of −70 mV by stepping the membrane potential to a series of potentials from –80 mV to +60 mV for 400 ms. Action potentials, under current-clamp condition, were evoked from the membrane potential of around −75 mV by injecting a series of current steps from −5 pA to 100 pA (in 5 pA increments). Data from some neurons in both WT and mutant lines, with 10 pA increments, or maximum current injection of less than 100 pA, were pooled with those obtained with the current injection protocol above since there was no difference. In current-clamp, we subtracted a liquid junction potential of 16 mV from the membrane potential values measured with K+-gluconate based intracellular solutions.
To investigate synaptic function, mEPSCs, in voltage-clamp, were recorded in human Ngn2 neurons at the holding membrane potential of −60 mV. Recording micropipettes were filled with an intracellular solution as described above. The extracellular recording solutions consisted of (in mM): 140 NaCl, 1 MgCl2, 5.4 KCl, 1.3 CaCl2, 25 glucose, 15 HEPES, 0.003 glycine, 0.001 strychnine, 0.01 bicuculline, 0.0005 tetrodotoxin, and pH was adjusted to 7.35 with NaOH. mEPSCs were detected and analyzed off-line using Mini Analysis Program (Synaptosoft Inc, USA).
Neuron sparse labeling
Neurons were transfected with pL-SIN-EF1α-eGFP plasmid (Addgene 21320) using 1 µg of DNA and 2 µL of Lipofectamine 2000 (Thermo Fisher Scientific) per well of a 24-well plate. 48 h post-transfection, neurons were fixed at 6 weeks and stained for MAP2 and GFP.
Immunocytochemistry and imaging
iPSCs were fixed with 4% formaldehyde in PBS for 8 min at room temperature. Neurons grown on 12 mm round glass coverslips (Bellco) or 24-well µ-Plates (Ibidi) were fixed with 4% formaldehyde in 0.4 M sucrose Krebs buffer for 8 min to preserve cytoskeletal integrity . Fixed cells were permeabilized with 0.1% Triton X-100 for 8 min at room temperature and blocked for at least 1 h at room temperature in ICC buffer (10% normal goat (Cedarlane) or donkey (Sigma) serum in PBS with 0.05% Tween 20). Primary antibodies were diluted in ICC buffer and incubated overnight at 4 °C. Secondary antibodies were diluted in PBS with 0.05% Tween 20 and incubated for 1 h at room temperature. Unless otherwise indicated, nuclei were counterstained with 1 µg/ml DAPI in PBS for 5 min at room temperature. If cells were on coverslips, they were rinsed in molecular grade water and mounted on slides in Prolong Gold antifade mounting media (Thermo Fisher). Details for primary antibodies used in this study can be found in Table S3.
iPSC pluripotency and 3-germ layer assays were imaged with a Leica DM14000B epifluorescence microscope using a DFC7000T camera and LAS X software. Neurons for soma/dendrite tracing were imaged with an Olympus 1 × 81 spinning disk confocal microscope using a Hamamatsu C9100-13 EM-CDD camera and Volocity software (Perkin Elmer). Synapses were imaged with a Leica SP8 Lightning Confocal/Light Sheet point scanning confocal microscope and LAS X software.
Blinded images were analyzed using Filament Tracer in Imaris (Bitplane) by two individuals. Soma were manually traced with semi-automated reconstruction of dendrites on sparsely GFP labeled neurons that were co-stained with MAP2 (3–5 replicate batches, with 4–6 coverslips per cell line in a batch, total = 404 neurons). Synapse density was manually counted for over-lapping SYN1 and HOMER1 puncta along a given length of MAP2 dendrite signal (2 replicate batches, with 4 coverslips per cell line in a batch, total = 240 dendrite segments).
MEA plating and recording
12-well CytoView MEA plates (Axion Biosystems) with 64 channels per well in 8 × 8 electrode grids were coated with filter sterilized 0.1% poly(ethylenimine) solution (Sigma) in borate buffer pH 8.4 for 1 h at room temperature, washed 4 times with water and dried overnight. Day 8 differentiated Ngn2 neurons were re-seeded at a density of 1 × 105 cells per 100 µL droplet in CM2 media consisting of BrainPhys (STEMCELL Technologies), 1x penicillin/streptomycin, 10 ng/ml BDNF and 10 ng/mL GDNF, supplemented with 400 μg/mL laminin and 10 μM ROCK inhibitor. Droplet of cells was applied directly on top of electrodes in each well, incubated for 1 h at 37 °C with hydration and then followed by slow addition of CM2 media supplemented with 10 μg/mL laminin. 24 h later, 2 × 104 P1 mouse astrocytes were seeded on top of the neurons in each well. CM2 media supplemented with 10 μg/mL laminin was changed consistently 24 h prior to recording twice per week from 2 weeks onwards on the Maestro MEA platform (Axion Biosystems). Each plate was incubated for 5 min on a 37 °C heated Maestro device, then recorded for 5 min of spontaneous neural activity using AxIS 2.0 software (Axion Biosystems) with a 0.2–3 kHz bandpass filter at 12.5 kHz sampling frequency. Spikes were detected at 6x the standard deviation of the noise on electrodes. Offline analysis used the Neural Metric Tool (Axion Biosystems), where an electrode was considered active if at least 5 spikes/min was detected. Poisson surprise burst and Envelope network burst algorithms with a threshold of at least 25% active electrode were applied. Further analyses and normalization of MEA metrics were completed in RStudio and MATLAB.
Statistical tests for assays were performed in RStudio (v1.1.423), SigmaPlot, GraphPad Prism, or MATLAB. D’Agostino & Pearson test was used to determine normality for datasets. Comparisons within respective isogenic pairs were conducted by two-way repeated measure ANOVA, Student’s t, one sample t or Mann–Whitney tests where appropriate (*p < 0.05, **p < 0.01, ***p < 0.001).
The network model consists of N adaptive leaky integrate-and-fire (ADIF) neurons. Consistent with the biological iPSC-derived neuronal cultures studied here, the network is fully excitatory. The membrane potential Vi of the ith unit evolves in time according to the equations:
where Cm is the membrane capacitance, gL is the leak conductance, EL is the resting membrane potential, ge is the time-dependent excitatory synaptic conductance, and Ee is the excitatory reversal potential. In the second equation, w is the adaptation variable, τw is the adaptation time constant, and a controls subthreshold adaptation.
When the membrane potential of neuron i exceeds threshold Vth, the neuron emits a spike, and the following reset conditions occur:
where Vr is the reset potential, we is the excitatory synaptic weight, S is the set of neurons postsynaptic to neuron i, and b is the adaptation step current. Following the initial conductance increase caused by an incoming spike, the excitatory synaptic conductance follows simple exponential decay:
where τe is the excitatory synaptic time constant. Populations of N = 1000 neurons were simulated for 300 seconds, representative of the in vitro culture recordings, with time-step of 0.1 ms in the Brian2 simulator . Neurons were randomly connected with excitatory synapses and a connection probability of 0.05, leading to an average of 50 synapses per cell in the simulated networks.
MEA raw voltage signals were measured for 300 s at a 12.5 kHz sampling frequency and Butterworth bandpass filtered between 200 Hz and 3000 Hz. Spike times were subsequently detected using an adaptive threshold crossing (threshold set at 6 × Std dev) and organized into spike trains per electrode, then further downsampled to 1 kHz. The power spectral density (PSD) of each spike train was estimated using MATLAB’s pwelch() function, and was expressed in decibels (dB) as a ratio against the PSD of a randomly permuted version of the same spike train. The power spectrum provides a numerical weight for each measurable frequency of an input time series. The described synchronous, regular bursting corresponded to the global peaks of the spectra between 0 Hz and 1 Hz. Spectra with global peaks outside this range were found to not clearly exhibit such bursting and were therefore excluded from analysis.
To identify the ideal time constant for adaptation (τw), we scanned a two-dimensional parameter space of capacitances (Cm) and neuronal membrane time constants (τm) in the range observed in patch-clamp recordings. Peak burst frequency in the simulations was evaluated through the same approach as the recorded culture data. Top simulations for the null and atypical CLT L124W conditions were determined as having the smallest absolute difference from the mean peak burst frequency of the recorded data.
To deconstruct the influence of various electrophysiologically-contributing variables to the changes in burst frequency, we performed a computational drop-out experiment. Here we changed either Na+/K+ currents or capacitance and resting membrane values, based on the reported values of the isogenic pair in Fig. 2, in the WIBR3 WT and WIBR3 null networks and calculated peak burst frequency as previously mentioned.
RNAseq datasets are available at GEO accession GSE148435. Code for visualizing electrophysiology, morphology, MEA analysis and simulation modelling is available at http://github.com/mullerlab/mok2021.
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We thank the family of the atypical patient for their participation; Lyndon Duong, Milad Khaki and Fraser McCready for preliminary MEA computational studies; Janice Hicks for the great technical support; Yun Li for the kind gift of WIBR3 WT and NULL cells; TCAG for karyotyping and sequencing; and the SickKids Imaging Facility.
This research was funded by grants from Col Harland Sanders Rett Syndrome Research Fund at the University of Toronto (to JE), SFARI (Research grant #514918 to JE and JM-T), CIHR (MOP-133423 to JE and MWS; ERARE Team Grant ERT161303 (to JE), CIHR foundation grant (FDN-154336 to MWS), Ontario Brain Institute (POND Network to JE), McLaughlin Centre Accelerator grant (to JE), John Evans Leadership Fund & Ontario Research Fund (to JE), Canada Research Chair in Stem Cell Models of Childhood Disease (to JE), Beta Sigma Phi International Endowment Fund (to JE), BrainsCAN at Western University through the Canada First Research Excellence Fund (CFREF) (to GB, KP, LM, JM-T), National Institutes of Health (NIH) grants # R01MH108528, R01MH109885, and R01MH1000175 (to ARM), and Ontario Rett Syndrome Association (to JBV). Trainee support was provided by NSERC Postgraduate Scholarship–Doctoral (PGS-D) Scholarship (to KP) and Restracomp (to LCD).
ARM is a co-founder and has equity interest in TISMOO, a company dedicated to genetic analysis and brain organoid modeling focusing on therapeutic applications customized for autism spectrum disorder and other neurological disorders with genetic origins. The terms of this arrangement have been reviewed and approved by the University of California San Diego in accordance with its conflict of interest policies. MRH became an employee of STEMCELL Technologies Inc during the preparation of this manuscript.
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Mok, R.S.F., Zhang, W., Sheikh, T.I. et al. Wide spectrum of neuronal and network phenotypes in human stem cell-derived excitatory neurons with Rett syndrome-associated MECP2 mutations. Transl Psychiatry 12, 450 (2022). https://doi.org/10.1038/s41398-022-02216-1