Fig. 4: CHRFAM7A modulates amyloid beta uptake via the α7 nicotinic acetylcholine receptor. | Translational Psychiatry

Fig. 4: CHRFAM7A modulates amyloid beta uptake via the α7 nicotinic acetylcholine receptor.

From: iPSC model of CHRFAM7A effect on α7 nicotinic acetylcholine receptor function in the human context

Fig. 4

a Difference in concentration-dependent Aβ1–42 uptake by MGE progenitors derived from UB068 and UB052 lines. Data are presented as mean ± SD. *P < 0.05 **P < 0.001—difference between Aβ1–42 uptake in UB052 line compared to UB068 at each given Aβ1–42 concentration. Inset: representative EVOS images of Aβ1–42 (100 nM) uptake in both cell lines. b Pretreatment with α7-selective antagonist methyllycaconitine (MLA; 10 μM) leads to a significant decrease in Aβ1–42 (100 nM) uptake in both cell lines. Data are presented as mean ± SD. *P < 0.05—difference between Aβ1–42 uptake in MGE progenitors with and without treatment (NT) with MLA. Transfection of UB068 (0 copy) with CHRFAM7A causes a decrease in Aβ1–42 uptake in a concentration-dependent manner compared to transfection with empty vector (EV) analyzed by cell counts (c) and as mean fluorescent intensity by flow cytometry (d). Data are presented as mean ± SD. *P < 0.05—difference between Aβ1–42 uptake in CHRFAM7A-transfected cells compared to EV-transfected cells at each given Aβ1–42 concentration

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