Fig. 3: miR-449a and miR-34c are downregulated in sperm across generations and in early embryos derived from them in a mouse model of early life stress. | Translational Psychiatry

Fig. 3: miR-449a and miR-34c are downregulated in sperm across generations and in early embryos derived from them in a mouse model of early life stress.

From: Reduced levels of miRNAs 449 and 34 in sperm of mice and men exposed to early life stress

Fig. 3

a qPCR analysis of miR-449a, miR-34c-5p, miR-152-3p, and miR-375-3p in pooled mature motile sperm isolated from stressed or control mice, n = 4–6 males per pool, 1 pool per group. bc qPCR analysis of miR-449a (b), miR-34c-5p (c) in pooled F1 embryos derived from mating control females with stressed or unstressed males. Embryos for each group of fathers and cell stage were pooled prior to analysis (n = 30–60 embryos per pool), 1 pool per group. d Same analysis as (a) performed on sperm from offspring of control and stressed fathers. All samples were pooled before triplicate qPCR analysis, presented as fold change (mean ± experimental error) normalized to a F0 control, b 2-cell control, c 2-cell control, d F0 control

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