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Association between endothelial nitric oxide synthase (NOS3) rs2070744 and the risk for migraine

The Pharmacogenomics Journal volume 20, pages 426–432 (2020)Cite this article

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Abstract

Because nitric oxide could play an important role in the pathogenesis of migraine (suggested by experimental, neuropathological, biochemical, and pharmacological data), and a recent meta-analysis showed an association between the single-nucleotide polymorphism (SNP) rs2070744 in the endothelial nitric oxide synthase (eNOS or NOS3) gene (chromosome 7q36.1) and the risk for migraine in Caucasians, we attempted to replicate the possible association between this SNP and the and the risk for migraine in the Caucasian Spanish population. The frequencies for the NOS3 rs2070744 genotypes and allelic variants were assessed in 283 migraine patients and 287 healthy controls with a TaqMan-based qPCR Assay. The putative influence on genotype frequency of age at onset of migraine attacks, gender, family history of migraine, absence or presence of aura, and triggering of migraine attacks by ethanol, were also analyzed. The frequencies of NOS3 rs2070744 genotypes and allelic variants were not associated with the risk for migraine (OR [95%] CI for the minor allele = 0.91 [0.72–1.15]) and were not influenced by age at onset of migraine, gender, presence of aura, or triggering of migraine attacks by ethanol. NOS3 rs2070744CC genotypes were significantly more frequent in patients with a family history of migraine. NOS3 rs2070744 SNP is not associated with the risk for migraine in Caucasian Spanish people although it might be related to family history.

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Acknowledgements

This work was supported in part by grants RETICS RD16/0006/0004 (ARADyAL), PI15/00303, and PI18/00540 from Fondo de Investigación Sanitaria, Instituto de Salud Carlos III, Madrid, Spain, and GR18145 and IB16170 from Junta de Extremadura, Mérida, Spain. Partially funded with FEDER funds.

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Authors and Affiliations

  1. University Institute of Molecular Pathology Biomarkers, UNEx, ARADyAL, Cáceres, Spain

    Elena García-Martín, Christopher Rodriguez & José A. G. Agúndez

  2. Section of Neurology, Hospital La Mancha-Centro, Alcázar de San Juan, Ciudad Real, Spain

    Santiago Navarro-Muñoz, Laura Turpín-Fenoll, Marta Recio-Bermejo, Rafael García-Ruiz & Jorge Millán-Pascual

  3. Department of Family Medicine, Hospital “Príncipe de Asturias”, Universidad de Alcalá, Alcalá de Henares, Madrid, Spain

    Mercedes Serrador

  4. Section of Neurology, Hospital Universitario del Sureste, Arganda del Rey, Madrid, Spain

    Hortensia Alonso-Navarro, Marisol Calleja, Francisco Navacerrada, José Francisco Plaza-Nieto & Félix Javier Jiménez-Jiménez

  5. Department of Medicine-Neurology, Hospital “Príncipe de Asturias”, Universidad de Alcalá, Alcalá de Henares, Madrid, Spain

    Esteban García-Albea

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García-Martín, E., Navarro-Muñoz, S., Rodriguez, C. et al. Association between endothelial nitric oxide synthase (NOS3) rs2070744 and the risk for migraine. Pharmacogenomics J 20, 426–432 (2020). https://doi.org/10.1038/s41397-019-0133-x

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