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Microbial controls over soil priming effects under chronic nitrogen and phosphorus additions in subtropical forests

The ISME Journal volume 17, pages 2160–2168 (2023)Cite this article

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Abstract

The soil priming effect (PE), defined as the modification of soil organic matter decomposition by labile carbon (C) inputs, is known to influence C storage in terrestrial ecosystems. However, how chronic nutrient addition, particularly in leguminous and non-leguminous forests, will affect PE through interaction with nutrient (e.g., nitrogen and phosphorus) availability is still unclear. Therefore, we collected soils from leguminous and non-leguminous subtropical plantations across a suite of historical nutrient addition regimes. We added 13C-labeled glucose to investigate how background soil nutrient conditions and microbial communities affect priming and its potential microbial mechanisms. Glucose addition increased soil organic matter decomposition and prompted positive priming in all soils, regardless of dominant overstory tree species or fertilizer treatment. In non-leguminous soil, only combined nitrogen and phosphorus addition led to a higher positive priming than the control. Conversely, soils beneath N-fixing leguminous plants responded positively to P addition alone, as well as to joint NP addition compared to control. Using DNA stable-isotope probing, high-throughput quantitative PCR, enzyme assays and microbial C substrate utilization, we found that positive PE was associated with increased microbial C utilization, accompanied by an increase in microbial community activity, nutrient-related gene abundance, and enzyme activities. Our findings suggest that the balance between soil available N and P effects on the PE,  was dependent on rhizosphere microbial community composition. Furthermore, these findings highlight the roles of the interaction between plants and their symbiotic microbial communities in affecting soil priming and improve our understanding of the potential microbial pathways underlying soil PEs.

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Fig. 1: SOM-derived CO2 and priming effects in different treatments.
Fig. 2: Principal co-ordinates analysis (PCoA) of the response ratio (RR) of bacterial and fungal communities among treatments (LP = leguminous plant, NLP = non-leguminous plant, CK = no nitrogen or phosphorus addition, N = nitrogen addition, P = phosphorus addition, and NP = nitrogen and phosphorus addition).
Fig. 3: Correlation between priming effect, soil and microbial properties.
Fig. 4: Conceptual diagram depicting factors and mechanisms underlying the soil priming effect.

Data availability

The sequencing raw data are deposited in the NCBI Short Read Archive database under accession numbers PRJNA884088 and PRJNA884089.

References

  1. Davidson EA, Janssens IA. Temperature sensitivity of soil carbon decomposition and feedbacks to climate change. Nature. 2006;440:165–73.

    Article  CAS  PubMed  Google Scholar 

  2. Lal R. Digging deeper: a holistic perspective of factors affecting soil organic carbon sequestration in agroecosystems. Glob Change Biol. 2018;24:3285–301.

    Article  Google Scholar 

  3. Li J, Alaei S, Zhou MY, Bengtson P. Root influence on soil nitrogen availability and microbial community dynamics results in contrasting rhizosphere priming effects in pine and spruce soil. Funct Ecol. 2021;35:1312–24.

    Article  CAS  Google Scholar 

  4. Rasul M, Cho J, Shin HS, Hur J. Biochar-induced priming effects in soil via modifying the status of soil organic matter and microflora: a review. Sci Total Environ. 2022;805:150304.

    Article  CAS  PubMed  Google Scholar 

  5. Cheng WX. Rhizosphere feedbacks in elevated CO2. Tree Physiol. 1999;19:313–20.

    Article  PubMed  Google Scholar 

  6. Kuzyakov Y, Friedel JK, Stahr K. Review of mechanisms and quantification of priming effects. Soil Biol Biochem. 2000;32:1485–98.

    Article  CAS  Google Scholar 

  7. Bastida F, García C, Fierer N, Eldridge DJ, Bowker MA, Abades S, et al. Global ecological predictors of the soil priming effect. Nat Commun. 2019;10:1–9.

    Article  CAS  Google Scholar 

  8. Huo CF, Luo YQ, Cheng WX. Rhizosphere priming effect: a meta-analysis. Soil Biol Biochem. 2017;111:78–84.

    Article  CAS  Google Scholar 

  9. Guenet B, Camino-Serrano M, Ciais P, Tifafi M, Maignan F, Soong JL, et al. Impact of priming on global soil carbon stocks. Glob Change Biol. 2018;24:1873–83.

    Article  Google Scholar 

  10. Morrissey EM, Mau RL, Schwartz E, McHugh TA, Dijkstra P, Koch BJ, et al. Bacterial carbon use plasticity, phylogenetic diversity and the priming of soil organic matter. ISME J. 2017;11:1890–9.

    Article  PubMed  PubMed Central  Google Scholar 

  11. Chen RR, Senbayram M, Blagodatsky S, Myachina O, Dittert K, Lin XG, et al. Soil C and N availability determine the priming effect: microbial N mining and stoichiometric decomposition theories. Glob Change Biol. 2014;20:2356–67.

    Article  Google Scholar 

  12. Feng J, Zhu B. Global patterns and associated drivers of priming effect in response to nutrient addition. Soil Biol Biochem. 2021;153:108118.

    Article  CAS  Google Scholar 

  13. Li J, Zhou M, Alaei S, Bengtson P. Rhizosphere priming effects differ between Norway spruce (Picea abies) and Scots pine seedlings cultivated under two levels of light intensity. Soil Biol Biochem. 2020;145:107788.

    Article  CAS  Google Scholar 

  14. Li J, Bengtson P. Comparative analysis of planted and unplanted controls for assessment of rhizosphere priming effect. Pedosphere. 2022;32:884–92.

    Article  CAS  Google Scholar 

  15. Liu XJA, Finley BK, Mau RL, Schwartz E, Hungate BA. The soil priming effect: consistent across ecosystems, elusive mechanisms. Soil Biol Biochem. 2020;140:107617.

    Article  CAS  Google Scholar 

  16. Blagodatskaya E, Kuzyakov Y. Mechanisms of real and apparent priming effects and their dependence on soil microbial biomass and community structure: critical review. Biol Fertil Soils. 2008;45:115–31.

    Article  Google Scholar 

  17. Lv C, Wang C, Cai A, Zhou Z. Global magnitude of rhizosphere effects on soil microbial communities and carbon cycling in natural terrestrial ecosystems. Sci Total Environ. 2022;856:158961.

    Article  PubMed  Google Scholar 

  18. Razanamalala K, Razafimbelo T, Maron P-A, Ranjard L, Chemidlin N, Lelièvre M, et al. Soil microbial diversity drives the priming effect along climate gradients: a case study in Madagascar. ISME J. 2018;12:451–62.

    Article  PubMed  Google Scholar 

  19. Tao X, Feng J, Yang Y, Wang G, Tian R, Fan F, et al. Winter warming in Alaska accelerates lignin decomposition contributed by Proteobacteria. Microbiome. 2020;8:1–12.

    Article  Google Scholar 

  20. Chen J, Sinsabaugh RL. Linking microbial functional gene abundance and soil extracellular enzyme activity: implications for soil carbon dynamics. Glob Chang Biol. 2021;27:1322–5.

    Article  CAS  PubMed  Google Scholar 

  21. Manoharan L, Kushwaha SK, Ahrén D, Hedlund K. Agricultural land use determines functional genetic diversity of soil microbial communities. Soil Biol Biochem. 2017;115:423–32.

    Article  CAS  Google Scholar 

  22. Trivedi P, Delgado-Baquerizo M, Trivedi C, Hu H, Anderson IC, Jeffries TC, et al. Microbial regulation of the soil carbon cycle: evidence from gene–enzyme relationships. ISME J. 2016;10:2593–604.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  23. Zhao F, Wang J, Li Y, Xu X, He L, Wang J, et al. Microbial functional genes driving the positive priming effect in forest soils along an elevation gradient. Soil Biol Biochem. 2022;165:108498.

    Article  CAS  Google Scholar 

  24. Song M, Guo Y, Yu F, Zhang X, Cao G, Cornelissen JHC. Shifts in priming partly explain impacts of long-term nitrogen input in different chemical forms on soil organic carbon storage. Glob Chang Biol. 2018;24:4160–72.

    Article  PubMed  Google Scholar 

  25. Fang XM, Zhang XL, Chen FS, Zong YY, Bu W-S, Wan S-Z, et al. Phosphorus addition alters the response of soil organic carbon decomposition to nitrogen deposition in a subtropical forest. Soil Biol Biochem. 2019;133:119–28.

    Article  CAS  Google Scholar 

  26. Liu W, Qiao C, Yang S, Bai W, Liu L. Microbial carbon use efficiency and priming effect regulate soil carbon storage under nitrogen deposition by slowing soil organic matter decomposition. Geoderma. 2018;332:37–44.

    Article  CAS  Google Scholar 

  27. Hessen DO. Carbon sequestration in ecosystems: the role of stoichiometry. Ecology. 2004;85:1179–92.

    Article  Google Scholar 

  28. Liebig JF. Animal Chemistry: Or, Organic chemistry in its applications to physiology and pathology. 1842. Johnson Reprint Corporation, New York, USA.

  29. Camenzind T, Hättenschwiler S, Treseder KK, Lehmann A, Rillig MC. Nutrient limitation of soil microbial processes in tropical forests. Ecol Monogr. 2018;88:4–21.

    Article  Google Scholar 

  30. Jobbágy EG, Jackson RB. The vertical distribution of soil organic carbon and its relation to climate and vegetation. Ecol Appl. 2000;10:423–36.

    Article  Google Scholar 

  31. Schulte‐Uebbing L, de Vries W. Global‐scale impacts of nitrogen deposition on tree carbon sequestration in tropical, temperate, and boreal forests: a meta‐analysis. Glob Change Biol. 2018;24:e416–31.

    Article  Google Scholar 

  32. Vitousek PM, Porder S, Houlton BZ, Chadwick OA. Terrestrial phosphorus limitation: mechanisms, implications, and nitrogen-phosphorus interactions. Ecol Appl. 2010;20:5–15.

    Article  PubMed  Google Scholar 

  33. Du E, Fenn ME, De Vries W, Ok YS. Atmospheric nitrogen deposition to global forests: status, impacts and management options. Envion Pollut. 2019;250:1044–8.

    Article  CAS  Google Scholar 

  34. Elrys AS, Zhu Q, Jiang C, Liu J, Sobhy HHH, Shen Q, et al. Global soil nitrogen cycle pattern and nitrogen enrichment effects: tropical versus subtropical forests. Glob Chang Biol. 2023;29:1905–21.

    Article  CAS  PubMed  Google Scholar 

  35. Elser JJ, Bracken ME, Cleland EE, Gruner DS, Harpole WS, Hillebrand H, et al. Global analysis of nitrogen and phosphorus limitation of primary producers in freshwater, marine and terrestrial ecosystems. Ecol Lett. 2007;10:1135–42.

    Article  PubMed  Google Scholar 

  36. Wang S, Zhou K, Mori T, Mo J, Zhang W. Effects of phosphorus and nitrogen fertilization on soil arylsulfatase activity and sulfur availability of two tropical plantations in southern China. Ecol Manag. 2019;453:117316.

    Article  Google Scholar 

  37. Lu J, Yang J, Keitel C, Yin L, Wang P, Cheng W, et al. Rhizosphere priming effects of Lolium perenne and Trifolium repens depend on phosphorus fertilization and biological nitrogen fixation. Soil Biol Biochem. 2020;150:108005.

    Article  CAS  Google Scholar 

  38. Phillips RP, Fahey TJ. Tree species and mycorrhizal associations influence the magnitude of rhizosphere effects. Ecology. 2006;87:1302–13.

    Article  PubMed  Google Scholar 

  39. Bhattacharyya PN, Jha DK. Plant growth-promoting rhizobacteria (PGPR): emergence in agriculture. World J Microbiol Biotechnol. 2012;28:1327–50.

    Article  CAS  PubMed  Google Scholar 

  40. Kawaka F. Characterization of symbiotic and nitrogen fixing bacteria. AMB Express. 2022;12:11.

    Article  Google Scholar 

  41. Zhang W, Zhu X, Luo Y, Rafique R, Chen H, Huang J, et al. Responses of nitrous oxide emissions to nitrogen and phosphorus additions in two tropical plantations with N-fixing vs. non-N-fixing tree species. Biogeosci Disc. 2014;11:1413–42.

    Google Scholar 

  42. Hicks LC, Leizeaga A, Rousk K, Michelsen A, Rousk J. Simulated rhizosphere deposits induce microbial N-mining that may accelerate shrubification in the subarctic. Ecology. 2020;101: e03094.

  43. Rousk J, Smith AR, Jones DL. Investigating the long-term legacy of drought and warming on the soil microbial community across five European shrubland ecosystems. Glob Chang Biol. 2013;19:3872–84.

    Article  PubMed  Google Scholar 

  44. Lu RK. Analytical methods of soil agrochemistry. Chinese Agriculture Science and Technology Press, Beijing 1999.

  45. Brookes PC, Landman A, Pruden G, Jenkinson DS. Chloroform fumigation and the release of soil-nitrogen-a rapid direct extraction method to measure microbial biomass nitrogen in soil. Soil Biol Biochem. 1985;17:837–42.

    Article  CAS  Google Scholar 

  46. Frostegård Å, Bååth E, Tunlio A. Shifts in the structure of soil microbial communities in limed forests as revealed by phospholipid fatty acid analysis. Soil Biol Biochem. 1993;25:723–30.

    Article  Google Scholar 

  47. Dumont MG, Murrell JC. Stable isotope probing-linking microbial identity to function. Nat Rev Microbiol. 2005;3:499–504.

    Article  CAS  PubMed  Google Scholar 

  48. Zhang K, Ni Y, Liu X, Chu H. Microbes changed their carbon use strategy to regulate the priming effect in an 11-year nitrogen addition experiment in grassland. Sci Total Environ. 2020;727:138645.

    Article  CAS  PubMed  Google Scholar 

  49. Neufeld JD, Vohra J, Dumont MG, Lueders T, Manefield M, Friedrich MW, et al. DNA stable-isotope probing. Nat Protoc. 2007;2:860–6.

    Article  CAS  PubMed  Google Scholar 

  50. Zhou J, Deng Y, Luo F, He Z, Yang Y. Phylogenetic molecular ecological network of soil microbial communities in response to elevated CO2. MBio. 2011;2:e00122–11.

    Article  PubMed  PubMed Central  Google Scholar 

  51. Ihrmark K, Bödeker I, Cruz-Martinez K, Friberg H, Kubartova A, Schenck J, et al. New primers to amplify the fungal ITS2 region–evaluation by 454-sequencing of artificial and natural communities. FEMS Microbiol Ecol. 2012;82:666–77.

    Article  CAS  PubMed  Google Scholar 

  52. Zheng B, Zhu Y, Sardans J, Penuelas J, Su J. QMEC: a tool for high-throughput quantitative assessment of microbial functional potential in C, N, P, and S biogeochemical cycling. Sci China Life Sci. 2018;61:1451–62.

    Article  CAS  PubMed  Google Scholar 

  53. Campbell CD, Chapman SJ, Cameron CM, Davidson MS, Potts JM. A rapid microtiter plate method to measure carbon dioxide evolved from carbon substrate amendments so as to determine the physiological profiles of soil microbial communities by using whole soil. Appl Environ Microbiol. 2003;69:3593–9.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  54. Marx MC, Wood M, Jarvis SC. A microplate fluorimetric assay for the study of enzyme diversity in soils. Soil Biol Biochem. 2001;33:1633–40.

    Article  CAS  Google Scholar 

  55. Saiya-Cork KR, Sinsabaugh RL, Zak DR. The effects of long term nitrogen deposition on extracellular enzyme activity in an Acer saccharum forest soil. Soil Biol Biochem. 2002;34:1309–15.

    Article  CAS  Google Scholar 

  56. Wild B, Li J, Pihlblad J, Bengtson P, Rütting T. Decoupling of priming and microbial N mining during a short-term soil incubation. Soil Biol Biochem. 2018;129:71–79.

    Article  Google Scholar 

  57. Heuck C, Weig A, Spohn M. Soil microbial biomass C: N: P stoichiometry and microbial use of organic phosphorus. Soil Biol Biochem. 2015;85:119–29.

    Article  CAS  Google Scholar 

  58. Widdig M, Schleuss P-M, Biederman LA, Borer ET, Crawley MJ, Kirkman KP, et al. Microbial carbon use efficiency in grassland soils subjected to nitrogen and phosphorus additions. Soil Biol Biochem. 2020;146:107815.

    Article  CAS  Google Scholar 

  59. Kuzyakov Y, Blagodatskaya E. Microbial hotspots and hot moments in soil: concept and review. Soil Biol Biochem. 2015;83:184–99.

    Article  CAS  Google Scholar 

  60. Bei S, Li X, Kuyper TW, Chadwick DR, Zhang J. Nitrogen availability mediates the priming effect of soil organic matter by preferentially altering the straw carbon-assimilating microbial community. Sci Total Environ. 2022;815:152882.

    Article  CAS  PubMed  Google Scholar 

  61. Mehnaz KR, Corneo PE, Keitel C, Dijkstra FA. Carbon and phosphorus addition effects on microbial carbon use efficiency, soil organic matter priming, gross nitrogen mineralization and nitrous oxide emission from soil. Soil Biol Biochem. 2019;134:175–86.

    Article  CAS  Google Scholar 

  62. Wu W, Wang F, Xia A, Zhang Z, Wang Z, Wang K, et al. Meta-analysis of the impacts of phosphorus addition on soil microbes. Agric, Ecosyst Environ. 2022;340:108180.

    Article  CAS  Google Scholar 

  63. Ilstedt U, Giesler R, Nordgren A, Malmer A. Changes in soil chemical and microbial properties after a wildfire in a tropical rainforest in Sabah, Malaysia. Soil Biol Biochem. 2003;35:1071–8.

    Article  CAS  Google Scholar 

  64. Jing Z, Chen R, Wei S, Feng Y, Zhang J, Lin X. Response and feedback of C mineralization to P availability driven by soil microorganisms. Soil Biol Biochem. 2017;105:111–20.

    Article  CAS  Google Scholar 

  65. Zhu Z, Ge T, Luo Y, Liu S, Xu X, Tong C, et al. Microbial stoichiometric flexibility regulates rice straw mineralization and its priming effect in paddy soil. Soil Biol Biochem. 2018;121:67–76.

    Article  CAS  Google Scholar 

  66. Ilstedt, Singh S. Nitrogen and phosphorus limitations of microbial respiration in a tropical phosphorus-fixing acrisol (ultisol) compared with organic compost. Soil Biol Biochem. 2005;37:1407–10.

    Article  CAS  Google Scholar 

  67. Delgado-Baquerizo M, Maestre FT, Reich PB, Jeffries TC, Gaitan JJ, Encinar D, et al. Microbial diversity drives multifunctionality in terrestrial ecosystems. Nat Commun. 2016;7:10541.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  68. Fu X, Song Q, Li S, Shen Y, Yue S. Dynamic changes in bacterial community structure are associated with distinct priming effect patterns. Soil Biol Biochem. 2022;169:108671.

    Article  CAS  Google Scholar 

  69. Zhou S, Wang J, Chen L, Wang J, Zhao F. Microbial community structure and functional genes drive soil priming effect following afforestation. Sci Total Environ. 2022;825:153925.

    Article  CAS  PubMed  Google Scholar 

  70. Blagodatskaya, Khomyakov N, Myachina O, Bogomolova I, Blagodatsky S, Kuzyakov Y. Microbial interactions affect sources of priming induced by cellulose. Soil Biol Biochem. 2014;74:39–49.

    Article  CAS  Google Scholar 

  71. Yang H, Li Y, Wang S, Zhan J, Ning Z, Han D. The response of critical microbial taxa to litter micro-nutrients and macro-chemistry determined the agricultural soil priming intensity after afforestation. Front Microbiol. 2021;12:730117.

    Article  PubMed  PubMed Central  Google Scholar 

  72. Xiao W, Chen X, Jing X, Zhu B. A meta-analysis of soil extracellular enzyme activities in response to global change. Soil Biol Biochem. 2018;123:21–32.

    Article  CAS  Google Scholar 

  73. Zechmeister-Boltenstern S, Keiblinger KM, Mooshammer M, Peñuelas J, Richter A, Sardans J, et al. The application of ecological stoichiometry to plant–microbial–soil organic matter transformations. Ecol Monogr. 2015;85:133–55.

    Article  Google Scholar 

  74. Lehmann J, Kleber M. The contentious nature of soil organic matter. Nature. 2015;528:60–8.

    Article  CAS  PubMed  Google Scholar 

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Acknowledgements

This study was supported by the National Natural Science Foundation of China (42207262 and 42177289) and the China Postdoctoral Science Foundation (2021M703135). JS was supported by Spanish Government grant. JP and JS were supported by the Spanish Government grants PID2020-115770RB-I00 and TED2021-132627B-I00 funded by MCIN, AEI/10.13039/501100011033 and the European NextGenerationEU/PRTR, the Fundación Ramón Areces grant CIVP20A6621, and the Catalan government project SGR2021-01333.

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Authors and Affiliations

  1. Key Laboratory of Vegetation Restoration and Management of Degraded Ecosystems & CAS Engineering Laboratory for Vegetation Ecosystem Restoration on Islands and Coastal Zones, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou, 510650, China

    Jian Li, Zhan-Feng Liu, Wei Zhang, Jing Zhang & Donghai Wu

  2. Key Laboratory of Urban Environment and Health, Ningbo Observation and Research Station, Institute of Urban Environment, Chinese Academy of Sciences, Xiamen, 361021, China

    Jian Li, Ming-Kang Jin & Yong-Guan Zhu

  3. Zhejiang Key Laboratory of Urban Environmental Processes and Pollution Control, CAS Haixi Industrial Technology Innovation Center in Beilun, Ningbo, 315830, China

    Jian Li, Ming-Kang Jin & Yong-Guan Zhu

  4. Guangdong Provincial Key Laboratory of Applied Botany, South China Botanical Garden, Guangzhou, 510650, China

    Zhan-Feng Liu, Jing Zhang & Donghai Wu

  5. School of Biological Sciences, University of Western Australia, Perth, WA, WA6009, Australia

    Hans Lambers

  6. Department of Plant Nutrition, College of Resources and Environmental Sciences; National Academy of Agriculture Green Development; Key Laboratory of Plan-Soil Interactions, Ministry of Education, China Agricultural University, Beijing, 100193, China

    Hans Lambers

  7. Department of Biological Sciences, Tennessee State University, Nashville, TN, TN37209, USA

    Dafeng Hui

  8. Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang, 110016, China

    Chao Liang

  9. CSIC, Global Ecology Unit, CREAF-CSIC-UAB, Bellaterra, 08193, Barcelona, Catalonia, Spain

    Jordi Sardans & Josep Peñuelas

  10. CREAF, Cerdanyola del Vallès, 08193, Barcelona, Catalonia, Spain

    Jordi Sardans & Josep Peñuelas

  11. Department of Ecology & Evolutionary Biology, Cornell University, Ithaca, NY, 14850, USA

    Daniel F. Petticord & David W. Frey

  12. State Key Laboratory of Urban and Regional Ecology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing, 100085, China

    Yong-Guan Zhu

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  1. Jian Li
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Contributions

ZFL, YGZ, and JL conceptualized the study and led the writing of the manuscript. JL, MKJ, WZ, and JZ performed the study and analyzed the data. HL, DFH, CL, DHW, JS, JP, DFP, and DWF contributed substantially to manuscript revisions. All authors reviewed and edited the manuscript.

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Correspondence to Zhan-Feng Liu or Yong-Guan Zhu.

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Li, J., Liu, ZF., Jin, MK. et al. Microbial controls over soil priming effects under chronic nitrogen and phosphorus additions in subtropical forests. ISME J 17, 2160–2168 (2023). https://doi.org/10.1038/s41396-023-01523-9

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