Search for homologs was performed with Ca. Cloacimonetes MAG sequences (arrows), in genomes from both cultures and environmental samples. Shadings illustrate the dominant group in the clade. Circles along branches indicate ultrafast bootstrap approximation support values, with only values for deepest nodes shown. See Fig. S6 and Fig. S7 for annotated trees with all branch support values. Scale bars represent mean number of substitutions per site. Asterisks in the DGGGP synthase tree indicate known UbiA prenyltransferases that do not have DGGGP synthase activity. A checkmark in the legend marks bacterial groups with genomes that code for both GGGP and DGGGP synthase, a gray checkmark marks groups in which both genes are found but not in the same genome. TACK includes the Thaumarchaeota and the Crenarchaeota. Archaeal or bacterial ‘phylum not known’: phylum is not known but the genome is annotated on a lower level, or sequence represents multiple groups. ‘Domain not known’: genomes for which no lineage was found on the PATRIC servers.