Fig. 2: The iPolony method detects viral infection throughout the infection cycle. | The ISME Journal

Fig. 2: The iPolony method detects viral infection throughout the infection cycle.

From: A single-cell polony method reveals low levels of infected Prochlorococcus in oligotrophic waters despite high cyanophage abundances

Fig. 2

Cultures of Synechococcus sp. strain WH8109 infected by the T7-like cyanopodovirus, Syn5 (a, c, e) and Prochlorococcus sp. strain MIT9515 infected by the T4-like cyanomyovirus, S-TIM4 (b, d, f) at MOI = 3. a, b Percent infection was determined using the polony method over the infection cycle. c, d Virus DNA replication (solid lines) and host genomic DNA degradation (dashed lines) were assessed by qPCR in infected cultures. Host and virus DNA concentrations were normalized to initial or maximum concentrations, respectively. Shaded regions indicate the period of virus genome replication. Lysis was assessed from an increase in plaque forming units measured by the plaque assay for Syn5 (e) or the appearance of extracellular virus DNA measured by qPCR for S-TIM4 (f). Note that Syn5 infections shown in (c) and (e) were not synchronized at 5 min post infection as in (a) and are shown for comparison of the timing of different phases of infection. Average and standard deviation of biological triplicates are shown in all panels.

Back to article page