Fig. 2: Taxonomic and phylogenetic analysis of the group 1h [NiFe]-hydrogenase large subunit (hhyL) genes in three soil ecosystems. | The ISME Journal

Fig. 2: Taxonomic and phylogenetic analysis of the group 1h [NiFe]-hydrogenase large subunit (hhyL) genes in three soil ecosystems.

From: Acidobacteria are active and abundant members of diverse atmospheric H2-oxidizing communities detected in temperate soils

Fig. 2

a Predicted taxonomic distribution of amplified hhyL sequences across the investigated soils based on their closest hits to sequences in the NCBI-nr database. Triplicate samples are depicted for each soil. Colors represent different taxonomic groups. b RAxML-EPA tree of amino acid sequences of the group 1h [NiFe]-hydrogenase large subunit (hhyL) from long-read amplicon sequencing and reference sequences. The phylogenetic placements of OTU representatives stemming from nearly full-length sequences are depicted in gray: collapsed clusters containing OTU representative are shaded gray and non-clustered OTU representatives are colored gray. The number of the placed, nearly full-length hhyL sequences and the total number of sequences in each cluster are depicted. The proportions of sequences within each soil are depicted to the right of the clusters managed grassland (red), rhizosphere (orange), beech forest (blue) and biological soil crust (purple). Sequences from group 1g [NiFe]-hydrogenases were used as an outgroup. The scale bar indicates the number of substitutions per site.

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