Fig. 2: Regulation of the metabolic activity of individual hyphal tips of P. involutus by the presence or absence of iron in lignin patches or liquid incubation media. | The ISME Journal

Fig. 2: Regulation of the metabolic activity of individual hyphal tips of P. involutus by the presence or absence of iron in lignin patches or liquid incubation media.

From: Regulation of fungal decomposition at single-cell level

Fig. 2

Principal component analysis was used to visually compare difference spectra across incubation times, patch types and experiments. a The first two principal components of difference spectra marked according to incubation time (days). b The first two principal components of difference spectra marked according to lignin patch type. “Lignin −Fe” denotes bare lignin patches; “Lignin+Fe” denotes lignin patches containing 1 mg Fe g−1 lignin in the form of ferrihydrite. c The first two principal components of difference spectra marked according to experiment. In experiment 1, liquid media contained 74 µM FeCl3•6H2O, resulting in mycelia with high overall oxidative decomposition activity, whereas in experiment 2, liquid media did not contain iron, resulting in mycelia with low overall oxidative decomposition activity. d The first two principal components of difference spectra marked according to clusters identified using model-based clustering. e Average difference spectra for each of the two clusters identified with model-based clustering. Lines above and below each measured wavenumber represent standard deviations (n = 92 for cluster 1 and n = 76 for cluster 2). Absorbance values equal to 0 indicate no difference in absorbance between hypha spectrum and background spectrum. Positive absorbance values indicate relative increases in absorbance values caused by the presence of hyphae and negative values indicate relative decreases in absorbance values caused by oxidative decomposition of lignin. In the inset, the spectral region from 1480 cm−1 to 1520 cm−1 is enlarged. Error bars were removed from the inset for clarity. Negative absorbance values in this range are indicative for oxidative lignin decomposition. Absorbance values are expressed in arbitrary units (a.u.). f Summary of the number of hyphae with high or low metabolic activity for each patch type and experiment.

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